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Flanking in Medical News

Farnesoid X receptor regulates cystathionase

...f FXR in the regulation of CSE transcription. The molecular mechanism of the CSE activation by FXR was revealed by identifying a sequence in the 5' flanking region of CSE gene containing a potential IR1 binding site. Co-transfection of HepG2 cells with luciferase reporter vector containing four copies of t...

Scientists reveal structure of new botulism nerve toxin subtype

... The current study of botulinum subtype E, also conducted at the NSLS, disproved that assumption, taking the scientists by surprise. Instead of the flanking arrangement, the binding and protein-cleaving domains of subtype E are both on the same side of the translocation domain. In addition, while all other...
Flanking in Biological News

200,000 rice mutants available worldwide for scientific investigation

... somewhere between 180,698 and 460,000. Two hundred thousand rice mutants are now available and have been mapped by the insertion of what are known as flanking sequence tags small pieces of DNA or molecular tags that integrate into the rice genome. This approach is useful because it allows scientists to link...

Pitt researchers describe molecular '2-step' leading to protein clumps of Huntington's disease

...the details of how the aggregation behavior of huntingtin depends, in a surprisingly intricate way, on the neighboring segments of amino acid sequence flanking the polyglutamine. They found that longer polyglutamine sequences have the ability to disrupt the structure of a neighboring region, 17 amino acids...

200,000 rice mutants available worldwide for scientific investigation

... somewhere between 180,698 and 460,000. Two hundred thousand rice mutants are now available and have been mapped by the insertion of what are known as flanking sequence tags small pieces of DNA or molecular tags that integrate into the rice genome. This approach is useful because it allows scientists to link...

CSHL scientists clarify editing error underlying genetic neurodegenerative disease

... exons -- are pasted together, forming a mature messenger RNA transcript. In an unedited RNA molecule, the boundaries between an intron and its two flanking exons are called splice sites. Such sites are composed of short sequences of RNA "letters" (called "bases"), which are referred to by single-letter m...

Tracking poultry litter phosphorus: Threat of accumulation?

... MADISON, WI, JANUARY 26, 2009 The Delmarva Peninsula, flanking the eastern shore of the Chesapeake Bay, is home to some 600 million chickens. The resulting poultry manure and some of the chicken house bedding mate...

Stowers Institute's Baumann Lab identifies key step in maturation pathway of telomerase

...paper. "It was highly unexpected that the spliceosome could have such a function, as the two steps of removing an internal piece of RNA and gluing the flanking ends together are tightly coupled during intron removal. To our knowledge this is the first example where uncoupling of the first and second step of s...

MU scientists 'see' how HIV matures into an infection

...protease precursor, something that had not been accessible before using conventional techniques. The researchers discovered that the 'tail,' or the flanking amino acid residues, of the HIV-1 protease precursor go through a temporarily formed tunnel where the tail is cut off. At this point, the protease bec...

A second career for a growth factor receptor: keeping nerve axons on target

...nce molecules that repel growing axons. In it, an immature neuron is placed on a microscopic running track, just as it starts to develop an axon. When flanking lanes are carpeted with repellant factors, the sprouting axon bursts from the block but remains in its lane like a well-coached runner, avoiding neigh...

Paper challenges 1491 Amazonian population theories

...te has major implications for land use and policy-setting in the rain forest. "We don't contradict that there were major settlements in key areas flanking the Amazon Channel -- there could have been millions of people living there," says Mark Bush, a British-born paleo-ecologist who travels to extremely...

Housecat-sized Siberian tiger cubs get collared

...he heart, face, and upper thorax. Dr. Bernice Morrow and her colleagues describe in this month's issue of Genome Research how they examined these flanking repetitive sequences for patterns of polymorphisms. "Our results show that there are intervals with more frequent traffic of genetic material ?region...
Flanking in Biological Technology

SNPs Powerful Tools for Association Studies

...sible to measure genetic variation. (Figure 1.) 1.1. What are SNPs? SNPs are single nucleotides that share identical flanking sequences and vary between individuals. These SNPs result from mutations that usually arose thousands of years ago and are stably passed down through ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

...ow a greater degree of site and size variation than coding regions. Introns may be amplified using conserved primers targeted to flanking exon sequence, a strategy termed exon-primed intron-crossing (EPIC) amplification. 7 When the size of a homologous intron varies among ...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...the predicted hairpin at its center and flanking genomic sequences of various sizes. (B... that native genomic sequences flanking each side of the predicted ha...miRNA transcripts lacking the flanking sequences resulted in accumul...

siRNA Design Guidelines

... using PCR-derived siRNA expression cassettes (SECs). SECs are PCR products that include promoter and terminator sequences flanking a hairpin siRNA template and can be prepared with Ambion's Silencer Express Kits . This screening strategy also permits the rapid ident...

Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient

...Each of the three exons of the LMP-1 gene was amplified separately using PCR3 for TA-cloning reactions. For this reason, primers flanking exactly the 5'-terminal and the 3'-terminal of each exon were synthesized in order that the cloned amplimer will be in-framed du...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

...A templates onto glass microscope slides. Such PCR products are typically generated using two vector-specific primers that anneal to priming sites flanking the cDNA insert. There have been many reports that the DNA spotted onto such microarrays is often a mixture of more than one clone or an incorrect...

Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector

...on stop codon would result in translational termination at the inserted sequence. Fast and Easy Subcloning Unique Pme I sites flanking the cloned cDNA can be used to subclone the RBS/Kozak and cDNA sequences into other vectors. Pme I is estimated to occur only once in 70,00...

Gene-Specific Primers for NMDA Receptor Subunits

... NR1, Rat; flanking C1 exon C1... NR1, Rat: flanking N1 exon N1...d. For NR1, amplification using primers flanking the C1 or N1 splice-variant exons from ...

The GeneZoo or the GeneJungle?

...alignable bases. The number of alignable bases is simply the sum of the local alignment length plus the length of unaligned sequence flanking the local alignment. Of course, alignments of commonly repeated or low complexity sequences are discarded. The algorithm will not cl...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...y PCR, and 41 (98%) were found to contain the JNK insert. The accuracy of the LIC cloning method was confirmed by sequence analysis of the regions flanking the N terminus and C terminus of the inserted gene. LIC cloning was also performed with 50 ng of prepared DNA coding for the kanamycin gene an...
Flanking in Biological Products

pTriEx™-4 DNA from Novagen

Description:...ls. Transient vertebrate expression is mediated by the CMV immediate early enhancer and promoter 2 . For expression in insect cells, pTriEx-4 contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector™ System. In baculovirus-infected insect cells, e...
Company:Novagen
Flanking in Biological Dictionary

UvrA

...Guidelines have been revised and agreed upon by all UVRA Town Directors. ... uvrA . Information for uvrA , and also upstream and downstream flanking ... Reported regulatory sites for uvrA : Genome coordinates. Source. Transcription factor ... Furthermore, the TFO was dissociated from its duplex...

Tet resistance

...all RP proteins, Tet (32) also confers resistance to minocycline (10 µg/ml) ... Analysis of tetracycline resistance tet (W) genes and their flanking sequences in ... Tetracycline Resistance Gene tet (W) in the Pathogenic Bacterium ... Mechanisms of resistance for characterized tet and otr...

Ribonucleotides

...ary ribonucleotides significantly augmented OVA-specific IFN- production by ... Ribonucleotides are indicated by gray boxes. ... The additional flanking ribonucleotides of the D10R2 and D9R3 substrates were ... Pol µ efficiently polymerizes ribonucleotides on a DNA primer. ... However, elongatio...

Retrotransposons

...EM, Liu J, ... LTR retrotransposons reveal recent extensive inter-subspecies nonreciprocal ... Some retrotransposons can also retrotranspose 3' flanking genomic sequences ... The impact of retrotransposons on genome size depends on both their rate of ... We previously characterized retrotranspo...

Multiple Cloning Site

... ... gene which is disrupted by ligation of a DNA insert into the cloning site . ... multiple cloning site with the double BglII site flanking the insertion site . ... Created by George Rice, Montana State University What is Cloning ? ... insertion site for the foreign DNA - also c...

Macronucleus

... ... 6000 loci in the developing macronucleus (YAO and GOROVSKY 1974; YAO et al. ... The wide, open boxes represent the macronucleus -retained flanking regions. ... ... called the macronucleus which controls ... Color the macronucleus red. ... 7. What do the macronucleus and micronucleus do...

Inverse PCR

...se PCR and Sequencing Protocol on 5 Fly Preps. For recovery of sequences flanking ... " Inverse PCR and Cycle Sequencing Protocols" by E. Jay Rehm. Berkeley ... Inverse PCR is used to clone sequences flanking a known sequence. ... For more PCR primer design tools, please visit Pr...

Flanking region

...is adjacent to 5' end of the gene. The 5' flanking region contains the promoter, and may contain enh...the University of Toronto have shown that the DNA flanking region in a family of neurological disorders is ... What is flanking regions ? Meaning of ...

Exons

...NA and in proteins. ... Statistical features of human exons and their flanking regions" ... Exons and Introns | World of Genetics. Exons and I...s that is transcribed ... Statistical features of human exons and their flanking regions" ... intron n. A segment of a gene situated between exons ...

Epitope

... provide consensus protein epitope prediction services and analysis that ... Epitope identity to databased protein sequences. Epitope (and flanking region) ... Last upload of Epitope . Liveleak requires flash. ... Uploads by user: ' Epitope ' - View all results in flash player. RNA transcript...
Other Tags
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