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Firefly in Medical News

Master molecular switch may prevent the spread of cancer cells to distant sites in the body

...so that each well of the plate expresses only one individual gene product. The Carstens lab developed a splicing "reporter" that makes cells express a firefly luciferase gene and "glow" when it is spliced in the epithelial cell pattern. Cells with this reporter were individually placed over wells containing ...

'Seeing' Stem Cells Helps in Fight Against Peripheral Arterial Disease

... by fireflies -- to "see" living stem cells. "We could 'see' the bubble via X-ray and tell if the stem cells remained alive as they lit up much like a firefly at night," said Kraitchman, who added that all agents used to form the bubble are approved by the Food and Drug Administration. The future hope ...

Cell Biosciences Introduces First Protein Analysis Kits

... the Firefly 3000(TM) Protein Analysis System. The firefly 3000 is an ultrasensitive nano-immunoassay system ...roteins in samples as small as 25 cells. The new firefly 3000 Focusing Kits, Detection Kits, and Plate Kits...iological samples. Our company's lead product, the firefly 3000, is a capillary-based nanofluidic immunoassay...

Good Habits for School Success

...rushing. Having a fun toothbrush geared toward children, such as the FireFly(R) from Dr. Fresh, can help promote good grooming and hygiene habits. The firefly blinks for 60 seconds so that kids know how long to brush each arch of teeth (one minute for the top, one minute for the bottom). The child simply pre...

The N Shapes Up This Summer With the New One-Hour Teen Reality Series, The N's Student Body

..., one of the world's foremost multinational entertainment and drama companies. The Shine Group also includes UK independent producers Kudos, Princess, firefly and Shine TV. About The N The N, the 24-hour TV network exclusively for and about teens, is currently available in 64 million households via cable...

Tracking Cancer Drug's Effectiveness

... team added a molecule substrate called luciferin to the bloodstream of mice with human breast cancer tumors. The tumors had been altered to carry the firefly gene, so they would emit light when the luciferin reached them. Using special light-detecting equipment, the researchers detected a correlation be...

Fireflies' glow helps UT Southwestern researchers track cancer drug's effectiveness

... the body. When luciferin reaches cells that have been altered to carry the firefly gene, those cells emit light. Some cancer drugs, however, work by cutti...ng introduced to the animals, the tumor cells had been transfected with the firefly gene, which becomes part of the cells as they divide and grow just like gen...

Fun a Factor for Children's Dental Care

...ren's toothbrush that lights up and flashes. The firefly toothbrush was developed when Dr. Fresh (yes, he i... be racing to add a dab of paste to his or her firefly and get brushing. The best way to impart lessons...lined to follow along. For more information on the firefly toothbrush and other Dr. Fresh fun products, visit...

Firefly genes allow testing of new therapy against lymphoma

... COLUMBUS, Ohio Researchers here have figured out a way to use a firefly gene to let them see just how effective a new drug combination actually is against some forms of cancer and its serious complication. The new stud...

PET and bioluminescent imaging aid evaluation of stem cells' potential for new ways to treat disease

... done since there are many things that we dont know about stem cell biology, noted Lee. For this study, researchers used a fusion protein combining firefly luciferase (a light-emitting substance) for optical imaging, a red fluorescent protein for cell separation and a virus enzyme thymidine kinase for PET...
Firefly in Medical Technology

Gene Therapy Eradicates Pancreatic Cancer in Preclinical Trial Targeting System's Success in Mouse Model Reported in Cancer Cell

...asis without having to kill and dissect the animal. They solved the monitoring problem by modifying two lines of pancreatic cancer to stably express firefly luciferace enzyme - the bioluminescent agent that lights up fireflies. This allowed live imaging of the mice to monitor cancer progression. The rese...
Firefly in Medical Products

SheerVision FireFly LED Light System

Description: The FireFly LED headlight provides superior Illumination in an ultra-light, fully portable package. With the FireFly you'll get bright center-beam light with a broad field of pure-white illumination wherever you direct it all totally hands free. The FireFly is engineered from the finest quality ma...
Company:SheerVision

FireFly Infinity Portable Headlight

Description:... The SheerVision firefly Infinity Light System offers the newest, brightest...vailable exclusively from SheerVision. With the firefly Infinity you'll get extremely bright, powerful, lo...uality materials to fit any loupe or headband, the firefly LED Light System comes with all the accessories yo...
Company:SheerVision

FireFly LED Light System & SheerVision 2.5x Titanium-Frame Loupe Package

Description:... The innovative & portable firefly LED light offers superior illumination in a lightweight, go-anywhere design. With the new firefly LED you'll get powerful, long-lasting illumination with unrestricted mobili...
Company:SheerVision
Firefly in Biological News

Biologists expose hidden costs of firefly flashes

MEDFORD/SOMERVILLE, Mass. -- A new study by biologists at Tufts University has discovered a dark side lurking behind the magical light shows put on by fireflies each summer. Using both laboratory and field experiments to explore the potential costs of firefly courtship displays, the biologists hav...

Tufts researchers shine light on firefly mysteries

This summer, in a darkened meadow west of Boston, Tufts University biologists are continuing to shine new light on the frenzied love life of fireflies. For the first time, researchers will explore the question of whether male fireflies' flashing light ?previously shown in one species to indicate...

American Chemical Society Weekly PressPac -- April 8, 2009

... release from ribosomes stops protein synthesis and is the mechanism of action of this deadly toxin. By coupling adenine release to light formation by firefly luciferase, scientists can visualize the presence of ricin by the simple detection of light. The test can detect nanogram (one-billionth of a gram) am...

Altered gene can increase risk of schizophrenia

...so added DNA that contained the gene that produces the enzyme which makes a firefly glow, along with human regulatory DNA which would control the production of that enzyme. The three kinds of DNA (the SNP, the firefly and the human regulatory) were all joined together prior to insertion into ...

Fireflies and jellyfish help illuminate quest for cause of infertility

... arthritis, as well as in the inflammation of cells and tissues. Scientists from the Universities of Edinburgh, Manchester and Liverpool harnessed firefly and jellyfish genes, which enable these creatures to emit light, and used them to create a chemical reaction to light up cells expressing prolactin in...

'Seeing' stem cells helps in fight against peripheral arterial disease

...uced by firefliesto "see" living stem cells. "We could 'see' the bubble via X-ray and tell if the stem cells remained alive as they lit up much like a firefly at night," said Kraitchman, who added that all agents used to form the bubble are approved by the Food and Drug Administration. The future hope is...

Scientists shed light on evolution of gene regulation

...e and reintroduced them into mouse red blood cells along with a gene from a firefly that is responsible for the insect's ability to produce light. The team's ... GATA1 would bind to the regulatory regions and turn on the activity of the firefly gene. "We wanted to know if the level of gene activity would change and,...

Small satellite takes on large thunderstorms

...attached end to end in a rectangular shape. The firefly team is a collaboration of scientists and students...tional technology of CubeSats to do just that. The firefly mission is a terrific example of a program that wi...he Earth's atmosphere." To accomplish these goals, firefly will carry a gamma-ray detector along with a suite...

Scientists identify novel inhibitor of human microRNA

...molecule, diazobenzene 2, decreased miR-21 levels by 80 percent and produced a nearly five-fold increase in the intensity of the light signal from the firefly protein. Groups of control cells and untreated cells showed no such signal when treated with the small-molecule inhibitor. The findings were publi...

Disrupting common parasites' ability to 'talk' to each other reduces infection

...lly and screened for low toxicity to animals. Scientists tested one of those herbicides against toxoplasmosis, labeling the test parasites with the firefly luciferase protein. Whole animal imaging showed that treatment with the herbicide reduced the number of parasites in infected mice during the initial ...
Firefly in Biological Technology

Cell Biosciences Introduces Kits for Oncoprotein Analysis

... MEK and ERK with a number of kinase inhibitor anticancer drugs. The firefly 3000 system is an ultrasensitive nanofluidic immunoassay system designed to...alysis in precious clinical samples. By using the new analysis kits on the firefly 3000 system, researchers can obtain detailed profiles of MEK and ERK signal...

Prosolia and Indigo BioSystems Come Together to Create FireFly(TM) Data Conversion Software for Mass Spectrometers

...using FireFly(TM) data conversion software. firefly is a software program that extracts and builds dat...ct on key decisions in biological R&D." firefly was created in order to visualize the multi-dimens...ardless of locale." For more information on firefly or Omni Spray Ion Sources contact Prosolia at 317....

Cell Biosciences Strengthens Management Team

...ation systems to enable the detection and characterization of specific proteins in extremely small biological samples. Our company's lead product, the firefly 3000, is a capillary-based nanofluidic immunoassay platform designed for ultrasensitive detection and characterization of proteins. Cell Biosciences i...

Cell Biosciences Announces New Leadership

...y small biological samples. Cell Biosciences began accepting orders for the firefly 3000 system in late 2007 as part of an early technology access program for ...eins in extremely small biological samples. The company's lead product, the firefly 3000, is a capillary-based nanofluidic immunoassay platform designed for ul...

Increased Light Leads to Early Detection: Caliper Life Sciences Introduces Bioware(R) Ultra Cancer Cell Lines

...e tumor growth and behaviors at the cellular level within living animals. By tagging a tumor with the gene for luciferase, the same gene that allows a firefly to glow, the tumor can produce light that can be monitored using Caliper's highly sensitive IVIS imaging systems. As the disease progresses and reacts...

Near Infrared Technology and Optical Agents For Molecular Imaging

... potential for high sensitivity and good spatial resolution. Bioluminescent imaging is an indirect technique based on the expression of firefly luciferase from recombinant plasmids inserted into hybrid cell lines that can be transplanted into animals. In some cases the gene is constituti...

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

...UCTION Luciferase from the North American firefly (Photinus pyralis) is very popular foruse in Repor... (Molecular Devices Corp.) 2. Recombinant firefly Luciferase, Sigma Cat # 9506. 3. CLIPR Lucife...serum albumin to Dulbeccos PBS 1X. 2. The firefly luciferase stock standard (1 mg/mL) was prepared i...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

...t bioluminescent reportersystems, especially using firefly luciferase, are becoming increasingly popularbecau...iferase Reporter Assay System which utilizes the firefly (Photinus pyralis) and Renilla (Renilla reniformis...both be measured in a single reaction mixture. The firefly luciferase is typicallyused as the experimental re...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

...minometer. PRINCIPLE OF ASSAY The basis for the assay is the reaction catalyzed by luciferase, obtained from the common American firefly (Photinus pyralis). 2 The enzyme catalyzes the ATP-dependent oxidation of luciferin with the concomitant release of light (Figure 1).When ATP is the ...

Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System

... for use in plants. Quantification of GFP is unlike widely used enzyme-based reporters such as β-glucuronidase (GUS) and firefly luciferase (LUC). We show here that fluorescence detection using the VersaFluor fluorometer system with appropriate narrow band ...
Firefly in Biological Products

Luciferase Reporter Assay Kit from MBL International

Description:... c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups firefly luciferase has been used as a sensitive reporter for studying gene regulati...n comparison to the standard chloramphenicol acetyltransferase (CAT) assay. firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction w...
Company:MBL International
Firefly in Biological Dictionary

Monocistronic

...n related to the first, how could ... 5 -UTR containing monocistronic reporter mRNAs continue ... monocistronic analysis of RNAs in vitro, the firefly luciferase ... or uncapped monocistronic ... GATA-4 5'-UTR sequences confer efficient translation of uncapped monocistronic reporter mRNAs. A...

Fusion Protein

...tigen-specific CTL responses and protect mice ... Immunohistochemical detection of His•Tag fusion proteins in transfected COS-1 cells ... Tag firefly luciferase (Fluc) fusion protein was transiently transfected into ... Affinity purification of GST fusion proteins ... PopCulture extractio...

C-terminus

.... ... Furthermore, we identified the C-terminus of Cx45.6 as being an essential ... The C-terminus of each enzyme is as follows, AOX, PH, and firefly luciferase,SKL; ... UOX has SRL, CAT has ANL, and DAOX has SHL at the C-terminus . ... C-terminus , we have further characterized the binding and...
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