Fundamentals of Fermentation: Techniques For Benchtop Fermentors
Part I - E. col
Prepared by the R & D Lab, New Brunswick Scientific Co., Inc.
The following document outlines step-by-step
procedures for carrying out a benchtop fermentation,
particularly when using a...
140-Fold Increase of Protein Achieved in Pichia Fermentation
...ch plagued earlier studies insufficient
oxygen, and a temperature increase of as much as 25C generated by the organisms prolific
growth. Although fermentors
commonly achieve yields of ten or more times that of shakers,
these extraordinary results were largely due to the unique capabilities inherent in th...
...cific nutrient solutions in the fermentor. Smaller fermentors
sterilized in an autoclave while larger fermentors
are sterilized by indirect or direct steam
injec...pores and 40% are Gram-negative bacteria.
generally work with aeration rates of 0.5-2 vvm (a...
The Genesis of Gendicine: The Story Behind the First Gene Therapy
...training in the operation of
equipment is essential for their customers to
be successful at reaching their fermentors
bioreactors full potential. To this end, the
company maintains in-house labs, fully
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
...sent in the medium at the time of inoculation. The fermentors
fed-batch mode further
enables methanol flow rat...r optimum protein production. In
addition to the fermentors
internal controller, an external bioprocessing sof... Research was conducted in BioFlo 3000 benchtop fermentors
( New Brunswick Scientific )
(Figure 1) with int...
Scaling-Up from Spinners, T-Flasks & Shakers: A versatile bioreactor for mammalian and microbial cells
... activate a peristaltic acid or base pump. Not all fermentors
handle the pH setpoint and
deadband control in t...act with gas bubbles. Agitation rates in microbial fermentors
are often set to 800 rpm
or more, providing both...ndamentals of Fermentation Techniques for Benchtop fermentors
Edison, NJ: New Brunswick Scientific...
Optimizing Your Benchscale Fermentation Process
...You can then calibrate
those devices through the fermentors
interface, as well as set a...e up to 32 parameters per
vessel, in one to four fermentors
than 120 process parameters...lo 310 is just one
of a series of fully scalable fermentors
and bioreactors offered
in 1 to 3,000-L capaciti...
An Easy-to-Use Research Fermentor/Bioreactor
...vessels and impellers. Optional data logging
and control software make this a powerful research tool. The
BioFlo 110 is just one in a full line of fermentors
bioreactors New Brunswick Scientific offers in 11500-L
capacities for all your research through production
P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
...age 5), cell density reached 177.4 g/L DCW.
Temperature control was excellent using unchilled (55F)
tap water as the coolant. Nevertheless, fermentors
large-area stainless-steel heat exchangers, such as the New
Brunswick Scientifics BioFlo 3000, have an advantage
in temperature control a...