Challenge the Performance of Your Hot-Start PCRs
with FastStart Taq DNA Polymerase and
the Novel FastStart High Fidelity PCR System
The
FastStart Taq DNA Polymerase
FastStart Taq DNA Polymerase, a chemically modified version
of Taq DNA Polymerase for hot start PCR, was introduced
in 2000. Based on the feedback f...
Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
...◊ CapSure HS LCM Caps (Arcturus, Cat. # LCM0214)
◊
faststart Taq DNA Poymerase (Roche, Cat. # 2 032 926)
◊ dNTP Mix PCR...To study expression of muscle specific genes, 50 ng cDNA are amplified with
faststart Taq DNA Polymerase for 40 cycles with conditions and reagents as presented ...
Housekeeping Genes: Universal Positive
Controls in siRNA Knockdown Experiments
... LC Instrument. For cDNA synthesis, the 1st Strand
cDNA Synthesis Kit was used, and quantitative real-time
PCR was performed using the LightCycler
faststart DNA Master Hybridization Probes Kit as described in the
preceding article (see pages 46).
Results
...
Gene Expression Arrays:
Highly Sensitive Detection of Expression Patterns
with Improved Tools for Target Amplification
...0 minutes, followed
by a 5-minute denaturation at 94 C. Two hundred picograms and 20 pg of cDNA, primers and hybridization
probes, and LightCycler
faststart DNA Master (as
described in the protocol) were used in a 20-l reaction.
The reaction conditions included: 10 minutes denaturation
at 95 C, 45 cy...
Quantification of siRNA Silencing Efficiency
Using the LightCycler System
...housekeeping gene amplification, the
LightCycler
faststart DNA Master SYBR Green I Kit and
LightCycler
faststart DNA Master Hybridization Probes
Kit were used ac...sekeeping Gene Selection
Set and the LightCycler
faststart DNA Master Hybridization Probes Kit. The data from...
Increase the Power and Sensitivity for Your cDNA
Synthesis with the New Transcriptor Reverse Transcriptase
...tal RNA is detected, in combination
with the new
faststart High Fidelity PCR System.
This enables the analy...PCR, use
FastStart Taq DNA Polymerase or the new
faststart High
Fidelity Enzyme Blend. Both allow hot-start... easily achieved (Figure 5). In combination
with
faststart Taq DNA Polymerase and the GC-RICH
Resolution So...
Roche Diagnostics Corporation
...llenge the Performance of Your Hot-Start PCRs with
faststart Taq DNA Polymerase and the Novel
faststart High Fidelity PCR System
...icult PCR Products from Small Amounts of DNA Using
faststart Taq DNA Polymerase
Increa...
Transcriptor Reverse Transcriptase
...r,
Transcriptor Reverse Transcriptase, the
faststart PCR Product Family, and
PCR-Grade Nucleoti...-PCR:
In combination with the new
faststart High
Fidelity PCR System, as little as 100...y and yield in the subsequent
PCR, use
faststart Taq DNA Polymerase or the new...
Product overview
...ication of very long DNA sequences.
faststart
Taq DNA Polymerase
Obtain su...x, nested, high-throughput,
or complex genomic PCR up to 3 kb with
faststart Polymerase. This modified
recombinant Taq DNA Polymerase is inacti...
FastStart Taq DNA Polymerase
...
Description
faststart Taq DNA Polymerase has been developed by Roche to ...ive in a convenient and rapid
way. With
faststart Taq DNA Polymerase, "Hot Start" PCR can
...w known "Hot
Start" methods.
faststart Taq DNA Polymerase is a thermostable, modified for...