FRT flanked, Pro and Euk. Neomycin Selection Cassette (FRT-PGK-gb2-neo-FRT) from Gene Bridges GmbH
Description:The with a eukaryotic promoter (PGK) for expression of neomycin resistance in mammalian cell...* Quick & Easy Conditional Knock Out Kit - FRT from Gene Bridges GmbH
Description:This kit is designed for the fast and simple integration of FRT-sites into large vector plasmids at any position (for the generation of transgenic mouse models). The included FRT-PGK-gb2-neo-FRT cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in with a eu...FRT flanked Ampicillin Selection Cassette (FRT-amp-FRT) from Gene Bridges GmbH
Description:The The prokaryotic promoter gb2 driving the gene for ampicillin resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the ampicillin expression. The cassette is flan...Flp-In pcDNA5/FRT Core Kit from Invitrogen
Description:Since the Flp-In System creates isogenic cell lines all transfected clones produce equivalent levels of the protein of interest. This homogeneous expression is demonstrated in Figure 3 (click link below). In this experiment the coding sequence for chloramphenicol-acetyl transferase (CAT) was subcloned into pcDNA5/FRT and transfected into Flp-In-293 cells. Western blot analysis of individual hygro...