Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
J. Samskog, H. Wadensten, and J. Flensburg GE Healthcare, Uppsala, Sweden A 2D–LC-MS method was developed to analyse phosphopeptides in mouse brain tissue. The trypsin-digested tissue was separated by strong cation exchange chromatography (SCX),...2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
Key words: phosphopeptide • two-dimensional liquid chromatography (2D-LC) • Ettan MDLC • LC-MS/MS • reversed-phase chromatography (RPC) • strong cation exchange (SCX) chromatography • linear ion trap mass spectrometer A 2D...The imager is controlled from the ettan DIGE Imager software, and can be set up for a variety of gel formats. Results from the ettan DIGE Imager are directly compatible with ImageQuan...
Extended data analysis using DeCyder EDA
...ull; base set • differential expression analysis • principal components analysis • pattern analysis • discriminant analysis • ettan DIGE DeCyder™ Extended Data Analysis (EDA) Software was used to perform advanced statistics on samples from a study on human ...