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Ethidium in Medical Products

HE 33 Mini Submarine unit, Complete

Description:...eapproximately 500 mland seal. Keep the base stored in the freezer. The UV transparent running tray can be placed on a transilluminator for viewing ethidium bromide stained bands. No taping required for casting. Small footprint13 cm wide x 24 cm long x 7 cm high. Electrical safety interlocks in the l...
Company:Hoefer, Inc.

HE 33 Mini Submarine unit, Basic

Description:...eapproximately 500 mland seal. Keep the base stored in the freezer. The UV transparent running tray can be placed on a transilluminator for viewing ethidium bromide stained bands. No taping required for casting. Small footprint13 cm wide x 24 cm long x 7 cm high. Electrical safety interlocks in the l...
Company:Hoefer, Inc.

RF-Mini 150 Fluorometer

Description:...ll as 1ng/ml of DNA specific Hoechst Dye. By switching to another set of excitation and emission filters, the RF-Mini 150 can measure RNA and DNA with ethidium bromide, or measure cell viability with fluorescein based tags. When samples are not available in large quantity, a volume as small as 3L can be measu...
Company:Shimadzu Scientific Instruments, Inc.

Mini ReadySub-Cell GT Cell

Features:... Tray locks into Sub-Cell GT cells Mini and wide mini formats Wide gel selection Three agarose percentages, TAE or TBE buffer, with or without ethidium bromide ...
Company:Bio-Rad Lab Equipment

Wide Mini ReadySub-Cell GT Cell

Features:... Tray locks into Sub-Cell GT cells Mini and wide mini formats Wide gel selection Three agarose percentages, TAE or TBE buffer, with or without ethidium bromide ...
Company:Bio-Rad Lab Equipment

OLERUP SSP™ HLA Kits

Features:...1) for HLA-A, HLA-B, and DRB. Complete line of accessory products for ease of use including a DNA size marker, negative control tubes, PCR wipe test, ethidium bromide dropper bottles and gel - electrophoresis equipment. Customized GenoVision version of HELMBERG-SCORE® Virtual Sequencing software availa...
Company:QIAGEN
Ethidium in Biological News

Researchers create new class of fluorescent dyes to detect reactive oxygen species in vivo

...n species, does not have. The dyes also have other advantages over DHE. "When DHE comes into contact with reactive oxygen species, it oxidizes into ethidium bromide, a common mutagen, which means it's toxic and can't be injected inside the body," explained Murthy. "DHE also auto-oxidizes in the presence of...
Ethidium in Biological Technology

Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

...Thus, it is important to test the RNA integrity. This has been traditionally performed by agarose gel electrophoresis and staining the RNA either with ethidium bromide or SYBR Green dye. In the past, the ratio between the ribosomal bands (28S:18S) was viewed as the primary indicator of RNA integrity, with a r...

Automation of the Eppendorf Perfectprep BAC 96 DNA Kit on the Caliper Life Sciences Sciclone ALH 3000

...TB Human BAC library plate #707 using the Eppendorf Perfectprep 96 kit. A volume of 10 μL from 24 samples were run on a 1% agarose gel containing ethidium bromide (Figure 2). Figure 2: BAC DNA isolated with the Eppendorf BAC 96 kit on the CaliperLS Sciclone ALH 3000. This shows 24 of the eluate...

LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

...tudy the performance of the LIVE/DEAD Viability/Cytoxicity Assay Kit for animal cells. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium homodimer (EthD-1), to stain live and dead cells simultaneously. Cal AM is an electrically neutral, nonfluorescent, esterase substrate that diffuses ...

Simple Approaches for Optimization of RT-PCR

...am, transfer tubes to ice. 7. Analysis Analyze sample (typically 1 to 10 μl aliquots) by agarose gel electrophoresis with staining by ethidium bromide. Visualize PCR product in gel with a UV transilluminator or fluorescence imager. Results and Discussio n ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...74; UltraPure TM 10x TAE (Invitrogen, Cat. # 15558-026) ◊ ethidium Bromide (Sigma, Cat. # E-1510) ◊ Tissue-Tek OCT Compound...2% agarose gel and separated by 120 V for 25 minutes. Gels are stained with ethidium bromide and visualized using the Gel Doc 2000 TM Gel Documentation System....

Oragene and Whole Genome Amplification with GenomiPhi

...l. The amplified DNA was quantified using the F/D Protocol. The molecular weight was analyzed by agarose gel electrophoresis with ethidium bromide staining and comparison with a Lambda-Hind III digest ladder. Polymerase Chain Reaction WGA and ...

DNA Stability with Oragene

... and processed by the standard Oragene protocol. 200 ng of DNA from each sample was analyzed by agarose gel electrophoresis with ethidium bromide staining. The molecular weight of the extracted DNA was determined by comparison with a Lambda-Hind III digest ladder. DN...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...; 89 mM boric acid, Mallinckrodt Inc., St. Louis, MO; 2 mM ethylenediamine tetraacetic acid, Fisher Scientific, Pittsburgh, PA). ethidium bromide (Bio-Rad Laboratories, Hercules, CA) was added to the agarose solution at a concentration of 0.5 mg/ml before the gel was ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...55 C and a temperature ramp of 1 C/hr. After electrophoresis, gels were equilibrated in 1.25x TAE buffer for 15 min., stained in ethidium bromide (25 mg/ml in TAE) for 20 min. and washed in TAE before viewing and photographing under UV light (302 nm). Various indivi...

Heteroduplex Analysis Using the DCode System

... electrophoresed on the DCode system at 100 V for 16 hours at room temperature. After electrophoresis, the gel was stained in 50 g/ml ethidium bromide in 0.6x TBE buffer for 5 minutes and destained in buffer for 10 minutes. The gel was imaged under UV transillumination. ...
Ethidium in Biological Products

SYBR Safe photographic filter from Molecular Probes (Invitrogen)

Description:...ilters for Fluorescent DyeStained Gels and Blots) is ideal for black-and-white photography of gels stained with the SYBR Safe DNA gel stain, the safer ethidium bromide alternative. Note that the SYBR Safe photographic filter is identical to the SYPRO photographic filter, which is described below....
Company:Molecular Probes (Invitrogen)

High Molecular Weight DNA Markers from Invitrogen

Description:...tage agarose gels. The 13 bands consist of 8.3- to 48.5-kb fragments generated from restriction endonuclease digests of Lambda DNA (cIind1ts857 Sam7). ethidium bromide staining causes the four bands identified by an asterisk (8271 bp 8612 bp 12220 bp and 19399 bp) to show greater intensity allowing them to be...
Company:Invitrogen

Supercoiled DNA Ladder from Invitrogen

Description:... Supercoiled DNA Ladder is suitable for sizing supercoiled DNA from 2 to 16 kb by agarose gel electrophoresis (1). The bands can be visualized by ethidium bromide staining and may also be detected on Southern blots by probing with the pBR322 Beta-lactamase (ampicillin-resistance) gene sequence....
Company:Invitrogen

EasyCount ViaSure Kit from Immunicon Corp.

Description:...ount dead and live nucleated cells automatically. The ViaSure Staining Reagent uses a mixture of ready-to-use nucleic acid dyes - Acridine Orange and ethidium Bromide to identify live and dead cells, respectively, in a single test, while the EasyCount Slide 6 Multi-well Slide provides the ability to perform...
Company:Immunicon Corp.

dsRNA Marker from Abnova Corporation

Description:...ntration. The dsRNA Marker is manufactured for non-denaturing polyacrylamide gel electrophoresis. The dsRNA Marker can be visualized by UV light after ethidium bromide staining....
Company:Abnova Corporation

UltraClean GelSpin (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:...er. Rapid Protocol - Purify DNA from agarose gels in as little as 5 minutes. Easy Format - Utilizes a silica spin filter method. Kit removes ethidium bromide, linkers and labels from TAE or TBE gels. User Friendly - Simply melt, bind, wash and collect DNA. Complete - All tubes and reagent...
Company:Mo Bio Laboratories, Inc.

DNA Visualizer Extraction Kit from Abnova Corporation

Description:... used for DNA-cloning work. The conventional method of visualizing DNA with ethidium bromide and exposure of UV light damages DNA and significantly decreases cl...f no UV damage, obtained DNA shows higher cloning efficiency than one using ethidium bromide and UV light. The Purified DNA with DNA Visualizer Extraction Kit ...
Company:Abnova Corporation

4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Sigma-Aldrich

Description:...y to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifica...
Company:Sigma-Aldrich

RNA Sample Loading Buffer from Sigma-Aldrich

Description:... ID clarifier: With ethidium bromide (50 μg/ml)...
Company:Sigma-Aldrich

RNA Sample Loading Buffer from Sigma-Aldrich

Description:... ID clarifier: Without ethidium bromide...
Company:Sigma-Aldrich
Ethidium in Biological Definition

Gel electrophoresis

...the electrophoresis run, when the smallest molecules have almost reached the anode, the molecules in the gel can be stained to make them visible. ethidium bromide , Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the ...

Gel electrophoresis

...the electrophoresis run, when the smallest molecules have almost reached the anode, the molecules in the gel can be stained to make them visible. ethidium bromide , Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the ...
Ethidium in Biological Dictionary

Gel electrophoresis

... for ... Agarose gel electrophoresis Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel , 3 Volts/cm, stained with ethidium bromide Experiment 2: Gel Electrophoresis of DNA. What is Electrophoresis ? Electrophoresis is a technique used in the laboratory ... Let's ...

Ethidium Bromide

... ethidium bromide is an intercalating agent commonly used a...ltraviolet light, it will fluoresce with an... ethidium bromide ( e′thidēəm ′brō′mīd ) ( organic chemis... melting point of 238-240°C; used in treating ethidium bromide is a large, flat basic molecule that ...

Agarose Gel

... ... Agarose gel electrophoresis Digital image of 3 plasmid restriction digests run on a 1% w/v agarose gel , 3 Volts/cm, stained with ethidium bromide The more agarose is dissolved in the boiling water, the firmer the gel will be. ... If you were inside an agarose gel , your enviro...
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