Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
...xylenes should be prepared fresh. Steps using 100% etoh are particularly sensitive, as small amounts of wa...re with microdissection. This lab changes the 100% etoh at least twice each day, and more often if the LCM...ing all 15 L extracts. An equivalent volume of 70% etoh was added to the pooled sample, and RNA isolation ...Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit
... Incubate at 37C for 1 hour. Add 5.0 l (0.1 vol) 5 M NaCl. Add 125 l (2.5 vol) cold 100% etoh and mix thoroughly. Incubate at -20C for 20-30 min. Centrifuge at top speed at 4C for 20 min. ...MMP-2 Inhibitor I from Calbiochem
Description:...T. PACKAGED UNDER INERT GAS. A potent inhibitor of MMP-2 that acts in a dose-dependent manner (K i = 1.7 μM). Purity: ≥98% by TLC. Soluble in etoh or DMSO. Unstable in solution, reconstitute just prior to use. M.W. 297.5. ...Cleland’s Reagent from Calbiochem
Description:...pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤1 ppm. Heavy metals: Oxidized dithiothreitol: ≤0.5%. Soluble in etoh or H 2 O. RTECS EK1610000, CAS 3483-12-3, M.W. 154.2. ...Cleland’s Reagent, ULTROL® Grade from Calbiochem
Description:... UNDER INERT GAS. Purity: ≥99% by titration. Heavy metals: Absorbance: (100 mM, 280 nm):≤0.05. Oxidized dithiothreitol:≤0.5%. Soluble in etoh or H 2 O. RTECS EK1610000, CAS 3483-12-3, M.W. 154.2. ...Cleland’s Reagent from Calbiochem
Description:...pentobarbitol. Purity: ≥97% by titration. Contaminants: Heavy metals: ≤1 ppm. Heavy metals: Oxidized dithiothreitol: ≤0.5%. Soluble in etoh or H 2 O. RTECS EK1610000, CAS 3483-12-3, M.W. 154.2. ...