UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
...ctly 30 minutes with constant agitation. Use a fresh dilution
for each gel. Do not destain the gel.
2. Place the gel on a glass...taining period
should be prolonged. DNA that is not stained with etbr
will not be nicked
by UV irradiation, and will not transfer effi...
DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
...n also be used to transfer RNA up to 3.5
kb in 3035 minutes, although transfer of 28s rRNA is equally efficient
as determined by etbr
Northern and Southern Blotting
The agarose gel support frame will accommodate 6 mm thick agarose gels
Get the 5'-End
...1) were analyzed using Ambion's RLM-RACE Kit. Initial
PCR (A) and nested PCR (B) products were analyzed on a 2% agarose gel
staining. The results indicate that a product of the expected
size is amplified after nested PCR. Note that CXCR4 is a moderately expressed...
Isolate Total RNA and Protein From the Same Sample
gel and stained with etbr
(top) or analyzed by solution hybridization
polyacryl-amide gel stained with etbr
(left top). Ten g of total protein
was ...a 1.2% denaturing glyoxal agarose gel stained with etbr
or resolved on
a 15% denaturing polyacr...
Improve RNA Isolation for Array Analysis and More
... 1% agarose gel in formaldehyde/MOPS buffer. Samples were heated at 80C
for 10 min in denaturing agarose gel loading buffer with 100 g/l etbr
before electrophoresis at 5 V/cm.
RiboPure-WBC: Scalable Protocol for Isolating High
Quality RNA from Bloo...
GenElute Minus EtBr Spin Column from Sigma-Aldrich
Description: The GenElute™ Minus EtBr
Spin Columns provide a rapid and simple method for recovering ethidium bromide (EtBr)-free DNA (100 bp to 10 kb) from stained agarose gels or EtBr
containing solutions. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the b...
Fujifilm LAS-3000 System Configured for Multifunctional Analysis from FUJIFILM Life Science
Description:...Filter Changer for LAS-3000
YBLAS3000TREPI EPI Tray for LAS-3000
YBLAS3000FLTGFP 510DF10 Long Pass Filter for GFP
YBLAS30000FLTEB 605 DF40 etbr
YBLAS3000WLI White Light Illuminator
YBLAS3000UVI UV Illuminator
YBLAS3000TRDIAUV UV DIA Tray for LAS-3000
Company:FUJIFILM Life Science