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EtBr in Biological Technology

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

...ctly 30 minutes with constant agitation. Use a fresh dilution of etbr for each gel. Do not destain the gel. 2. Place the gel on a glass...taining period should be prolonged. DNA that is not stained with etbr will not be nicked by UV irradiation, and will not transfer effi...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

...n also be used to transfer RNA up to 3.5 kb in 3035 minutes, although transfer of 28s rRNA is equally efficient as determined by etbr staining. 1 Northern and Southern Blotting The agarose gel support frame will accommodate 6 mm thick agarose gels ...

Get the 5'-End

...1) were analyzed using Ambion's RLM-RACE Kit. Initial PCR (A) and nested PCR (B) products were analyzed on a 2% agarose gel with etbr staining. The results indicate that a product of the expected size is amplified after nested PCR. Note that CXCR4 is a moderately expressed...

Isolate Total RNA and Protein From the Same Sample

...aturing acrylamide gel and stained with etbr (top) or analyzed by solution hybridization ...turing polyacryl-amide gel stained with etbr (left top). Ten g of total protein was ...a 1.2% denaturing glyoxal agarose gel stained with etbr or resolved on a 15% denaturing polyacr...

Improve RNA Isolation for Array Analysis and More

... 1% agarose gel in formaldehyde/MOPS buffer. Samples were heated at 80C for 10 min in denaturing agarose gel loading buffer with 100 g/l etbr before electrophoresis at 5 V/cm. RiboPure-WBC: Scalable Protocol for Isolating High Quality RNA from Bloo...
EtBr in Biological Products

GenElute Minus EtBr Spin Column from Sigma-Aldrich

Description: The GenElute™ Minus EtBr Spin Columns provide a rapid and simple method for recovering ethidium bromide (EtBr)-free DNA (100 bp to 10 kb) from stained agarose gels or EtBr containing solutions. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the b...
Company:Sigma-Aldrich

Fujifilm LAS-3000 System Configured for Multifunctional Analysis from FUJIFILM Life Science

Description:...Filter Changer for LAS-3000 YBLAS3000TREPI EPI Tray for LAS-3000 YBLAS3000FLTGFP 510DF10 Long Pass Filter for GFP YBLAS30000FLTEB 605 DF40 etbr Filter YBLAS3000WLI White Light Illuminator YBLAS3000UVI UV Illuminator YBLAS3000TRDIAUV UV DIA Tray for LAS-3000 YBLAS3000TRDIAWL Whi...
Company:FUJIFILM Life Science
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