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Enterokinase in Biological Technology

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...cognition target for the site-specific protease, enterokinase (EK). The EK target sequence is located between ... Figure 3 enterokinase Cleavage of the CBP Affinity Tag The pCALnEK v...s free of contaminating proteases. Each order of enterokinase is provided with Soybean Trypsin Inhibitor Agaro...

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

... Recombinant enterokinase for highly specific digestion of purified protei...et. Cleavage of fusion proteins that contain the enterokinase (EK) recognition sequence positioned between an ...the fusion protein. The serine protease enterokinase (EK) is particularly attractive because ...

Yeast Protein Production System Features High Yields and One-Step Purification

...protein by proteolytic cleavage with either bovine enterokinase or thrombin. As numerous genes that are involv... that are specifically recognized and cleaved by enterokinase and thrombin. These sites are located b... Removing the GST Tag by Proteolytic Cleavage with enterokinase or Thrombin In many cases, GST tags do n...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...eous amino acid fused to the protein of interest. The use of the protease enterokinase (EK) has the advantage that it cleaves at the C-terminus of its recogniti...y tag followed by a thrombin cleavage site, the FLAG epitope, an enterokinase cleavage site, the multiple cloning site (MCS), and the T7 trans...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...ately downstream of the GST affinity tag and the enterokinase (EK) recognition site, resulting in a seamless j...ESP-2 vector results in a seamless junction of the enterokinase site and the initiation codon of the ge...n protein and removal of the purification tag by enterokinase cleavage yield polypeptides with completely nati...

A New C-Terminal GST Vector for Protein Production in S. pombe

...oding for the recognition and cleavage sites for enterokinase and thrombin have been engineered between the MC...moved by incubating the fusion protein with either enterokinase or thrombin ( figures 2 Pane... can be obtained after removing the GST tag by enterokinase cleavage. 3,4 Both the pESP-1 and pESP...
Enterokinase in Biological Products

pCDF-1 Expression System plus Competent Cells from Novagen

Description:... plus Competent Cells contains 10 µg of pET-45b(+) DNA. pET-45b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ cod...
Company:Novagen

Enterokinase from Novagen

Description:... Recombinant enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine e...ing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl 2 .• 50 U Recombinant enterokinase • 2 ml 1X rEK Dilution/Storage Buffer • 1 ml 10X rEK Cleavage Buf...
Company:Novagen

Enterokinase from bovine intestine from Sigma-Aldrich

Description:... Physical Form: powder Color: white Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins. Supplied with optimized enterokinase buffer....
Company:Sigma-Aldrich

Enterokinase from Novagen

Description:... Recombinant enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site...
Company:Novagen

Enterokinase EKMax from Invitrogen

Description:...KMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ enterokinase is expressed and isolated from the yeast Pichia pastoris to yield a highly ...
Company:Invitrogen

pTriEx™-4 DNA from Novagen

Description:...y cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or Sma I sites and cleavage of the fusion protein with enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit . 1 patent pending 2 The CMV promoter is covered by U.S...
Company:Novagen

pET GST Fusion System 41 from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen

pET GST Fusion System 41 plus Competent Cells from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen

pET GST Fusion System 42 from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen
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