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Enterokinase in Biological Technology

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

...cognition target for the site-specific protease, enterokinase (EK). The EK target sequence is located between ... Figure 3 enterokinase Cleavage of the CBP Affinity Tag The pCALnEK v...s free of contaminating proteases. Each order of enterokinase is provided with Soybean Trypsin Inhibitor Agaro...

Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini

... Recombinant enterokinase for highly specific digestion of purified protei...et. Cleavage of fusion proteins that contain the enterokinase (EK) recognition sequence positioned between an ...the fusion protein. The serine protease enterokinase (EK) is particularly attractive because ...

Yeast Protein Production System Features High Yields and One-Step Purification

...protein by proteolytic cleavage with either bovine enterokinase or thrombin. As numerous genes that are involv... that are specifically recognized and cleaved by enterokinase and thrombin. These sites are located b... Removing the GST Tag by Proteolytic Cleavage with enterokinase or Thrombin In many cases, GST tags do n...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...eous amino acid fused to the protein of interest. The use of the protease enterokinase (EK) has the advantage that it cleaves at the C-terminus of its recogniti...y tag followed by a thrombin cleavage site, the FLAG epitope, an enterokinase cleavage site, the multiple cloning site (MCS), and the T7 trans...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...ately downstream of the GST affinity tag and the enterokinase (EK) recognition site, resulting in a seamless j...ESP-2 vector results in a seamless junction of the enterokinase site and the initiation codon of the ge...n protein and removal of the purification tag by enterokinase cleavage yield polypeptides with completely nati...

A New C-Terminal GST Vector for Protein Production in S. pombe

...oding for the recognition and cleavage sites for enterokinase and thrombin have been engineered between the MC...moved by incubating the fusion protein with either enterokinase or thrombin ( figures 2 Pane... can be obtained after removing the GST tag by enterokinase cleavage. 3,4 Both the pESP-1 and pESP...
Enterokinase in Biological Products

pCDF-1 Expression System plus Competent Cells from Novagen

Description:... plus Competent Cells contains 10 µg of pET-45b(+) DNA. pET-45b carries an N-terminal His•Tag ® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ cod...
Company:Novagen

Enterokinase from Novagen

Description:... Recombinant enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine e...ing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl 2 .• 50 U Recombinant enterokinase • 2 ml 1X rEK Dilution/Storage Buffer • 1 ml 10X rEK Cleavage Buf...
Company:Novagen

Enterokinase from bovine intestine from Sigma-Aldrich

Description:... Physical Form: powder Color: white Removes FLAG peptide from N-terminal and Met-N-terminal fusion proteins. Supplied with optimized enterokinase buffer....
Company:Sigma-Aldrich

Enterokinase from Novagen

Description:... Recombinant enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site...
Company:Novagen

Enterokinase EKMax from Invitrogen

Description:...KMax™ is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ enterokinase is expressed and isolated from the yeast Pichia pastoris to yield a highly ...
Company:Invitrogen

pTriEx™-4 DNA from Novagen

Description:...y cloning into the Nco I site, or native protein can be generated by cloning into the Psh AI or Sma I sites and cleavage of the fusion protein with enterokinase or Thrombin, respectively. The pTriEx-4 vector is also available as an Ek/LIC Cloning Kit . 1 patent pending 2 The CMV promoter is covered by U.S...
Company:Novagen

pET GST Fusion System 41 from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen

pET GST Fusion System 41 plus Competent Cells from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen

pET GST Fusion System 42 from Novagen

Description:...HisTag (6 aa) and STag (15 aa) sequences. In contrast to other commercially available GST-fusion vectors, the Xa (IleGluGlyArg, pET-42 series) and enterokinase (AspAspAspAspLys, pET-41 series) proteolytic cleavage sites have been engineered to allow 100% complete removal of vector encoded sequences and the ge...
Company:Novagen
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(Date:4/23/2014)... at the National Institute of Arthritis and Musculoskeletal and ... of the Ross Prize in Molecular Medicine, conferred by ... . The award will be given on June 9 ... followed by scientific presentations by Dr. O,Shea and other ... of Health. , The award, which includes a $50,000 ...
(Date:4/23/2014)... pathway that helps prevent new cells from receiving too ... been directly linked to cancer and other diseases. , ... the end of cell division, the enzyme Cdc14 activates ... are fully repaired before the parent cell distributes copies ... safeguard against some of the most devastating genome errors, ...
(Date:4/23/2014)... methods that can rapidly screen a large cell ... inside that population has been seriously lacking," said ... the Royal Society of Chemistry journal Chemical ... his coworkers have developed a novel technique that ... Chem. Sci. , 2014, DOI: 10.1039/C4SC00578C, http://pubs.rsc.org/en/content/articlelanding/2014/sc/c4sc00578c#!divAbstract ...
(Date:4/23/2014)... 21, 2014 Five for-profit and non-profit organizations ... million in Proof of Concept grants to accelerate ... commercial products, the Life Sciences Discovery Fund (LSDF) ... Mentoring Program grant to the Washington Biotechnology & ... network to train the next generation of entrepreneurs ...
(Date:4/23/2014)... depletion of self-control, according to research published in ... Psychological Science. , Self-control can be difficult sticking ... a boring textbook are hard things to do. Considerable ... self-control for a long period seems to "deplete" our ... "It is as if self-control is a limited resource ...
Breaking Medicine News(10 mins):Health News:NIH scientist to receive Ross Prize in Molecular Medicine 2Health News:Study identifies enzymes that help fix cancer-causing DNA defects 2Health News:Following a protein's travel inside cells is key to improving patient monitoring, drug development 2Health News:LSDF announces commercialization grants and Entrepreneur Mentoring Program 2Health News:ADHD drug may help preserve our self-control resources 2
(Date:4/23/2014)... German . Man ... difference between sexes depends on one single element of the genome: ... two sexual chromosomes are X and Y, whereas women have two ... morphological and physiological differences between males and females. , But this ... the X and Y were identical, until the Y started to ...
(Date:4/23/2014)... Huntington,s disease, most in their mid-thirties and forties, ... behavioral decline within two decades. "Mutant" protein clusters, ... disease, have been the primary focus of therapies ... new research from Prof. Gerardo Lederkremer and Dr. ... Cell Research and Immunology, in collaboration with Prof. ...
(Date:4/23/2014)... (Apr. 23 2014) A team of ... cells (hNSCs) into the brains of nonhuman primates ... and 24 months found that the hNSCs had ... not cause tumors. , The study will be ... Transplantation but is currently freely available on-line ...
Breaking Biology News(10 mins):Male or female? 2Male or female? 3On the defensive 2On the defensive 3Study finds long-term survival of human neural stem cells transplanted into primate brain 2
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