Electrophoretic

Farnesoid X receptor regulates cystathionase

...s this question. The research team led by Dr. Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, electrophoretic Mobility Shift Assay (EMSA), Chromatin Immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the regulation of CSE transcription. ...

Electrophoretic in Medical Products

Mini Trans-Blot Electrophoretic Transfer Cell

Description: Includes 2 gel holder cassettes, 4 fiber pads, modular electrode assembly, Bio-Ice cooling unit, lower buffer chamber, lid with cables, and instructions. The Mini Trans-Blot cell, a component of Bio-Rad's modular mini electrophoresis system (see Mini-Vertical Electrophoresis Systems), provides rap...
Company:Bio-Rad Lab Equipment

Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

Description: The Trans-Blot SD semi-dry transfer cell allows fast, efficient and economical blotting. Using a platinum-coated titanium plate as the anode and a stainless steel plate as the cathode, the Trans-Blot SD cell transfers in a horizontal configuration without a buffer tank or gel cassettes....
Company:Bio-Rad Laboratories

Criterion Dodeca Cell

Description:...an the traditional mini gel. They accommodate Bio-Rad's 11 cm ReadyStrip IPG strips to give you the optimal combination of separation and speed in 2-D electrophoretic applications. Precast gels and handcasting options are available....
Company:Bio-Rad Lab Equipment

High Voltage Non-Programmable Power Supplies

Description:... From SDS-PAGE to IEF to DNA sequencing, Thermo Series 90 high voltage power supplies will handle all routine electrophoretic applications. These power supplies can operate in constant voltage, constant current or constant power operating modes, with automatic crossover. To ...
Company:ThermoFisher Scientific

Agarose MP

Description:...is (PFGE)]. Agarose MP is a high gel strength agarose ( 1800 g/cm2, 1%). The high gel strength permits the use of low concentrations of agarose in electrophoretic gels. The agarose matrix of gels made with low concentrations of agarose MP allows rapid migration of high molecular weight molecules without signific...
Company:Roche Applied Science

UltraClean™ Agarose

Description:... UltraClean Agarose, Molecular Biology Grade, is used for the electrophoretic separation of nucleic acids. It can be used to isolate DNA fragments from 0.2 to 40 kb. Each lot of UltraClean Agarose is tested and certified to be ...
Company:Mo Bio Laboratories, Inc.

Certified Megabase Agarose

Description:... With a very high gel strength, high electrophoretic mobility, and high exclusion limit, Certified megabase agarose is the superior choice for CHEF and FIGE applications. The gels remain easy to handle a...
Company:Bio-Rad Lab Equipment

Certified Molecular Biology Agarose

Description:...ditional agaroses. In addition to the very low sulfate content, this produces a gel with a very high gel strength and higher exclusion limit. The high electrophoretic mobility increases resolution and reduces run time, and the gels are easy to handle even at low agarose percentages....
Company:Bio-Rad Lab Equipment

Liposure Human Serum Lipoprotein Control

Description:... This control is specifically designed for monitoring the performance of the Lipoprint LDL electrophoretic system for separation and quantification of cholesterol in various lipoprotein fractions and subfractions in patient serum samples. Liposure is assaye...
Company:Quantimetrix Corporation

GENIE BLOTTER (Will blot gels up to 15 x 17 cm. Us

Description:... The GENIE is the proven electrophoretic transfer apparatus incorporating state-of-the-art plate electrodes for the ...eates a high field in volts/cm. The result is exceptionally rapid and exact electrophoretic transfer. ...
Company:Idea Scientific

Electrophoretic in Biological News

Researchers create freestanding nanoparticle films without fillers

...hem is described in the article "Sacrificial layer electrophoretic deposition of freestanding multilayered nanopartic...tedly. They are also made using a technique called electrophoretic deposition (EPD) that is well suited for creating ...new form of EDP, which they call sacrificial layer electrophoretic deposition. They added a spun-cast layer of polyme...

Biological motors sort molecules one by one on a chip

...trical field is largely dependent on the orientation of the cylinder-shaped molecules. This was the first time that this orientation-dependency of the electrophoretic mobility was observed. In an accompanying Perspective article in Science, Professor Hess of the University of Florida wrote that the Delft resear...

Electrophoretic in Biological Technology

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

Kenneth D. Cain 1 , Leslie Grabowski 1 , and Scott E. LaPatra 2 1 Department of Fish and Wildlife Resources, University of Idaho, Moscow, ID USA 2 Clear Springs Foods Inc., Research Division, PO Box 712, Buhl, ID USA ...

DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell

The Trans-Blot SD DNA/RNA agarose gel support frame, used with the Trans-Blot SD semi-dry electrophoretic transfer cell, allows blotting of DNA/RNA from agarose gels to nylon membrane in minutes, without any gel pretreatments. ...

Caliper Life Sciences Introduces Zephyr Genomics Workstation for Automated Liquid Handling

...customer need for automated molecular biology applications. This new workstation complements our LabChip GX system, which performs fast, automated 1-D electrophoretic separations of nucleic acid samples. Caliper also offers Sciclone, Twister, and Staccato liquid handling and integrated system solutions for higher th...

Technical Note: E-PAGE(TM) 48- and 96-Well Gels on the Odyssey(R) Infrared Imaging System

...brane in as little as 40 minutes. Alternatively, a semi-dry transfer apparatus may be used for E-PAGE gel transfer, such as the Trans-Blot(R) Semi-Dry electrophoretic Transfer Cell (Bio-Rad Laboratories, Hercules, CA). Detection for blots processed in this manner can be performed using IRDye(R) Infrared Seco...

'Two-Faced' Particles Act Like Tiny Submarines

...: The application of ac electric fields in aqueous suspensions of anisotropic particles leads to unbalanced liquid flows and nonlinear, induced-charge electrophoretic motion. We report experimental observations of the motion of Janus microparticles with one dielectric and one metal-coated hemisphere induced by unifo...

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

...Laikin, Lens Design, Marcel Dekker, New York, 1991. 52. E. K. Seto, S. Damaskinos, A. Dixon, W. L. Diehl-Jones, and C. A. Mandato, "Imaging electrophoretic gels with a scanning beam laser macroscope," Electrophoresis 16 , pp. 934-940, 1995. 53. A. E. Dixon, S. Damaskinos, A. Ribes, and K. M....

High Throughput Plasmid DNA Preparation Using A Magnetic Bead Kit On An Automated Workstation

...ut of 384 samplings (4 x 96 well plates). Nucleic acid yield for the two systems are roughly 16 ng/μL by PicoGreen binding analysis. Results from electrophoretic separation of the purified nucleic acid are presented in Figure 2. Discussion The Agencourt CosMCPrep Kit...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

...lecule that is sufficient to be detected on a non-denaturing polymer, e.g., electrophoretic mobility differences compared with wild-type (wt) sequences. ...lowed by rapid chilling to prevent re-annealing of the strands; electrophoretic separation of the single-stranded DNA on a nondenaturing sieving medium, su...

ECL Semi-dry Blotters

... TE 70, TE 77, TE 70 PWR, TE 77 PWR ECL™ Semi-dry Blotters are designed to deliver outstanding electrophoretic transfer results using low current and voltage with a minimal amount of buffer. • Units available with and without built-in power ...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

...d for 2.5 h at 100 V using the miniVE Vertical Electrophoresis System.

Protein blotting and fluorescent detection After electrophoretic separation the gels were blotted onto either Hybond ECL (low-fluorescent nitrocellulose) or Hybond-LFP (low-fluorescent PVDF) membranes for 2.5 h at 2...

Electrophoretic in Biological Products

LIGHTSHIFT EMSA Kit from Pierce Biotechnology, Inc.

Description:... The electrophoretic mobility shift assay (EMSA) is a powerful tool for evaluating DNA-protein i...d oligos from custom oligo suppliers Sensitive, fast and non-radioactive electrophoretic mobility shift assays...
Company:Pierce Biotechnology, Inc.

ProteoExtract Protein Precipitation Kit from Calbiochem

Description:...e analysis is often hampered by the presence of non-protein impurities such as buffers, salts and detergents in the protein sample that interfere with electrophoretic separations or enzymatic digestion. Additionally, proteins in solutions are often too dilute for direct downstream applications such as 2DGE. Precipit...
Company:Calbiochem

Mini Trans-Blot and PowerPac Basic Power Supply from Bio-Rad

Description:... The Mini Trans-Blot cell and PowerPac Basic power supply are used for electrophoretic blotting from gels to membranes. The Mini Trans-Blot cell accommodates two 7.5 x 10 cm gels and includes 2 gel holder cassettes, modular electrode ass...
Company:Bio-Rad

The Discovery Series PDQuest Version Upgrade from Bio-Rad

Description:... The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The version upgrade allows upgrade of an established version of the PDQuest software to the newest version....
Company:Bio-Rad

PDQuest 10-User Network License from Bio-Rad

Description:... The Discovery Series PDQuest 2-D analysis software is used for imaging, analyzing, and databasing two-dimensional electrophoretic gels. The 10-user network license entitles ten users to access data from a server....
Company:Bio-Rad

2% Bis Solution, 500 ml from Bio-Rad

Description:... Bis-Acrylamide (bis) is a monomer used as a crosslinker with acrylamide to form polyacrylamide electrophoretic gels. This 2% bis solution is supplied as 500 ml....
Company:Bio-Rad

Stirbar, Trans-Blot Plus cell from Bio-Rad

Description:... The stirbar is used in the buffer tank of the Trans-Blot Plus cell to maintain uniform conductivity and temperature during electrophoretic transfer. Dimensions are 3.5 x 0.5 in....
Company:Bio-Rad

Super Cooling Coil, Trans-Blot cell from Bio-Rad

Description:... The super cooling coil, Trans-Blot cell, provides temperature regulation for native enzyme, high-intensity, and extended electrophoretic transfer (blotting) applications performed with the Trans-Blot cell. Use of the super cooling coil requires connection to an external water recirculat...
Company:Bio-Rad

ATTO-TAG CBQCA Amine-Derivatization Kit from Molecular Probes (Invitrogen)

Description:...CBQCA 1) and ATTO-TAG FQ (3-(2-furoyl) quinoline-2-carboxaldehyde 2) react specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. Unlike OPA and NDA, ATTO-TAG CBQCA will also react with hydrophilic peptides and amino sugars. The resulting products of C...
Company:Molecular Probes (Invitrogen)

ATTO-TAG CBQCA derivatization reagent (CBQCA; 3-(4-carboxybenzoyl)quinoline-2-carboxaldehyde) from Molecular Probes (Invitrogen)

Description:...CBQCA 1) and ATTO-TAG FQ (3-(2-furoyl) quinoline-2-carboxaldehyde 2) react specifically with primary amines to form conjugates that can be analyzed by electrophoretic or chromatographic methods. Unlike OPA and NDA, ATTO-TAG CBQCA will also react with hydrophilic peptides and amino sugars. The resulting products of C...
Company:Molecular Probes (Invitrogen)

Electrophoretic in Biological Dictionary

Western blotting

... The fi rst step in a Western ... In western blotting (or immunoblotting), prior to protein immobilization on the ... Western blotting ": electrophoretic transfer of proteins from sodium dodecyl ... A Western blot is a method in molecular biology to detect a certain protein in a ... Some hi...

SDS-PAGE

... electrophoresis, is a technique widely used in biochemistry, forensics, genetics and molecular biology to separate proteins according to their electrophoretic mobility .The... SDS polyacrylamide gel electrophoresis ( ¦es¦dē¦es ′pälēə¦kriləmīd ′jel i′lektrōfə′rēsəs ) ... 3 Reducing SDS - PAGE . 4 El...

Polyacrylamide Gel

... electrophoresis, is a technique widely used in biochemistry, forensics, genetics and molecular biology to separate proteins according to their electrophoretic mobility .The... polyacrylamide gel n. A hydrated polyacrylamide of stiff consistency used as a medium for electrophoresis because of its w...

Native Gel

...e the proteins are not denatured and therefore separated based on their charge-to-mass ratio. Native polyacrylamide gel electrophoresis is an electrophoretic separation method typically used in proteomics and metallomics. Blue Native Gel Electrophoresis. Stock solutions. 49.5%T, 3%C Acrylamide ... ...

Gel shift assay

... An electrophoretic mobility shift assay (EMSA) or mobility shift ...s that contain target ... The gel shift , or electrophoretic mobility shift , assay provides a simple and ra...n" or " gel mobility shift " assay . ... The electrophoretic gel shift assay is used to detect sequence ...

Blotting

...observe cellular control over structure ... Protein blotting is an analytical method that involves the immobilization of ... Western blotting ": electrophoretic transfer of proteins from sodium dodecyl ... Southern blotting is designed to locate a particular sequence of DNA within a complex mixture. ....

Band shift assay

... An electrophoretic mobility shift assay (EMSA) or mobility shift ...el, EMSA) - also called band shift assay , or electrophoretic mobility shift assay (EMSA), a ... Band ...y refers to an ideal ... The gel shift , or electrophoretic mobility shift , assay provides a simple and ra...