Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems
... a study. The Applied Biosystems Plant Mapping Kit contains eight selective ecori
primers for regular plant genomes, eight selective ecori
primers for small plant genomes and eight MseI primers. The primers are lab...
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
final size of 591 bp. In each case, 10 l of the PCR product was digested
in a volume of 30 l with either AvaII (apoA-I) or ecori
1 shows the melting profile graphs for the 5' and 3' fragments generated
after digestion of the apoB PCR product....
Transformation of Filamentous Fungi by High-Voltage Electroporation
...ted DNA, as well as DNA digested with EcoRV, BamHI, HindIII, and
was subjected to agarose gel electrophoresis and analyzed by Southern
...RV sites, one site each for BamHI and HindIII, and two sites
(which yields two fragments of about 3.9 and 2.6 kb). The digestions
Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
...ng a 32 P-dCTP
labeled mouse probe (a gift of Steven McKnight). Phage DNA was isolated
using a standard protocol, digested with ecori
and HindIII and run on
a 1.2% agarose gel (Figure 1). Bands corresponding to insert DNA were
excised and the DNA isolated on gla...
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
... efficiency with both bacterial and yeast hosts,
and sequencing. Restriction digests were done on 500 ng of plasmid in
20 l with ecori
(NEB) for 2 hr at 37C. Digests were resolved on 0.7%
agarose TAE gels. Bacteria were transformed as described above. Yeast
High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures
(10 pg/l) were obtained from Bio-Rad Laboratories. Linear pUC18 plasmid
was prepared by digestion of the supercoiled form with ecori
enzyme (New England Biolabs), followed by extraction with phenol/chloroform,
ethanol precipitation, and resuspension...
Efficient Recovery of Ultrapure Plasmid DNA
Fig. 1: Restriction digestion
of 40 individual plasmids with ecori
(Roche Molecular Biochemicals).
Vector pVL1392; length: 9.6 kb; bacteria strain: XL-1 Blue; medium
2x YT. The plasmid DN...
... Patterns: A Tool for Comparing Microbial Communities and Assessing Community Dynamics ... Terminal restriction fragment patterns ... A 1000 bp ecori
restriction fragment contains a gene you are interested in. ... Draw a restriction map of the fragment and show the distances, in base pai...
... Restriction enzymes were discovered 40 years ago during investigations into the ... Among the first restriction enzymes to be purified were ecori
and EcoRII from ... Restriction endonucleases are enzymes that cleave the sugar-phosphate backbone of DNA. ... The presence of restriction e...