Navigation Links
EcoRI in Biological Technology

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

... a study. The Applied Biosystems Plant Mapping Kit contains eight selective ecori primers for regular plant genomes, eight selective ecori primers for small plant genomes and eight MseI primers. The primers are lab...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...ith a final size of 591 bp. In each case, 10 l of the PCR product was digested in a volume of 30 l with either AvaII (apoA-I) or ecori (apoB). Figure 1 shows the melting profile graphs for the 5' and 3' fragments generated after digestion of the apoB PCR product....

Transformation of Filamentous Fungi by High-Voltage Electroporation

...ted DNA, as well as DNA digested with EcoRV, BamHI, HindIII, and ecori was subjected to agarose gel electrophoresis and analyzed by Southern ...RV sites, one site each for BamHI and HindIII, and two sites for ecori (which yields two fragments of about 3.9 and 2.6 kb). The digestions ...

Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer

...ng a 32 P-dCTP labeled mouse probe (a gift of Steven McKnight). Phage DNA was isolated using a standard protocol, digested with ecori and HindIII and run on a 1.2% agarose gel (Figure 1). Bands corresponding to insert DNA were excised and the DNA isolated on gla...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

... efficiency with both bacterial and yeast hosts, and sequencing. Restriction digests were done on 500 ng of plasmid in 20 l with ecori (NEB) for 2 hr at 37C. Digests were resolved on 0.7% agarose TAE gels. Bacteria were transformed as described above. Yeast were ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...A (10 pg/l) were obtained from Bio-Rad Laboratories. Linear pUC18 plasmid was prepared by digestion of the supercoiled form with ecori restriction enzyme (New England Biolabs), followed by extraction with phenol/chloroform, ethanol precipitation, and resuspension...

Efficient Recovery of Ultrapure Plasmid DNA

... Fig. 1: Restriction digestion of 40 individual plasmids with ecori (Roche Molecular Biochemicals). Vector pVL1392; length: 9.6 kb; bacteria strain: XL-1 Blue; medium 2x YT. The plasmid DN...
EcoRI in Biological Definition

Restriction enzyme

...strain) I First identified Order ID'd in bacterium Examples Enzyme Source Recognition Sequence Cut ecori Escherichia coli 5'GAATTC 5'---G AATTC---3' 3'CTTAAG 3'---CTTAA ...
EcoRI in Biological Dictionary

Restriction fragment

... Patterns: A Tool for Comparing Microbial Communities and Assessing Community Dynamics ... Terminal restriction fragment patterns ... A 1000 bp ecori restriction fragment contains a gene you are interested in. ... Draw a restriction map of the fragment and show the distances, in base pai...

Restriction enzymes

... Restriction enzymes were discovered 40 years ago during investigations into the ... Among the first restriction enzymes to be purified were ecori and EcoRII from ... Restriction endonucleases are enzymes that cleave the sugar-phosphate backbone of DNA. ... The presence of restriction e...
Other Tags
(Date:8/1/2015)... ... 01, 2015 , ... Brig and Lita Hart, icons of the network marketing ... Healthy Home mission with the HOPE Movement of EvolvHealth. In what has been a ... have agreed to join their missions of purpose together to change the course of ...
(Date:8/1/2015)... Dallas, TX (PRWEB) , ... August 01, 2015 , ... ... support for the March4thforWellBeing foundation, making hers the very first small business donation to ... and look beautiful for over thirty years. Her legacy includes a complete line of ...
(Date:7/31/2015)... ... July 31, 2015 , ... Ticket Down is a reputable source of authentic tickets for ... Arena. Fans of every genre of music will have an act they are excited ... of the festival, the following artists will perform: Sam Smith, Coldplay, Kenny Chesney, The ...
(Date:7/31/2015)... ... 31, 2015 , ... Ticket Down is a reliable source for authentic tickets ... Grant Park. The festival will end on Sunday, August 2nd. , ... annual Lollapalooza. This unique and unbelievably popular festival attracts the best in entertainment ...
(Date:7/31/2015)... ... 31, 2015 , ... The 2nd annual Ride to Fight On will start ... 3, 2015. The Ride to Fight On is a cycling fundraiser that benefits the ... West Clinic and The University of Tennessee Health Science Center. A health and wellness ...
Breaking Medicine News(10 mins):Health News:Looking for HOPE? Healthy Home Has Evolv’d 2Health News:Looking for HOPE? Healthy Home Has Evolv’d 3Health News:March4thforWellBeing Foundation Kickoff 2Health News:Cheap 2015 iHeartRadio Music Festival Tickets in Las Vegas at MGM Grand Garden Arena: Ticket Down Slashes Ticket Prices on the iHeart Radio Music Festival in Vegas 2Health News:Cheap 2015 iHeartRadio Music Festival Tickets in Las Vegas at MGM Grand Garden Arena: Ticket Down Slashes Ticket Prices on the iHeart Radio Music Festival in Vegas 3Health News:Cheap 2015 Lollapalooza Tickets: Ticket Down Slashes Ticket Prices on Lollapalooza Tickets at Grant Park in Chicago for Saturday, August 1st and Sunday, August 2nd 2Health News:West Cancer Center Announces 2nd Annual Ride to Fight On 2Health News:West Cancer Center Announces 2nd Annual Ride to Fight On 3
(Date:7/8/2015)... , July 8, 2015 Summary Pancreatic ... the fourth most fatal, with a mortality rate of ... prognosis of pancreatic cancer patients has highlighted a significant ... is not being met by the current market. ... of products with varying molecule types and mechanisms of ...
(Date:7/7/2015)... 2015 Research and ... of the "Capacitive Fingerprint Sensors Patent Landscape" ... this date, fingerprint sensing technology is the most ... are well developed. This patent landscape focuses on ... The domain of capacitive fingerprint sensors is closely ...
(Date:7/2/2015)... DUBLIN , June 25, 2015 ... the addition of the "Next Generation Biometrics Market ... to 2020" report to their offering. ... $24.4 Billion by 2020, at a CAGR of 17.9% ... is the leading application for the market. Safran ...
Breaking Biology News(10 mins):Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3
Other Contents