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EcoRI in Biological Technology

Amplified Fragment Length Polymorphism (AFLP) Analysis on Applied Biosystems Capillary Electrophoresis Systems

... a study. The Applied Biosystems Plant Mapping Kit contains eight selective ecori primers for regular plant genomes, eight selective ecori primers for small plant genomes and eight MseI primers. The primers are lab...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...ith a final size of 591 bp. In each case, 10 l of the PCR product was digested in a volume of 30 l with either AvaII (apoA-I) or ecori (apoB). Figure 1 shows the melting profile graphs for the 5' and 3' fragments generated after digestion of the apoB PCR product....

Transformation of Filamentous Fungi by High-Voltage Electroporation

...ted DNA, as well as DNA digested with EcoRV, BamHI, HindIII, and ecori was subjected to agarose gel electrophoresis and analyzed by Southern ...RV sites, one site each for BamHI and HindIII, and two sites for ecori (which yields two fragments of about 3.9 and 2.6 kb). The digestions ...

Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer

...ng a 32 P-dCTP labeled mouse probe (a gift of Steven McKnight). Phage DNA was isolated using a standard protocol, digested with ecori and HindIII and run on a 1.2% agarose gel (Figure 1). Bands corresponding to insert DNA were excised and the DNA isolated on gla...

Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A

... efficiency with both bacterial and yeast hosts, and sequencing. Restriction digests were done on 500 ng of plasmid in 20 l with ecori (NEB) for 2 hr at 37C. Digests were resolved on 0.7% agarose TAE gels. Bacteria were transformed as described above. Yeast were ...

High Electrotransformation Efficiencies Obtained With DNA From Ligation Mixtures

...A (10 pg/l) were obtained from Bio-Rad Laboratories. Linear pUC18 plasmid was prepared by digestion of the supercoiled form with ecori restriction enzyme (New England Biolabs), followed by extraction with phenol/chloroform, ethanol precipitation, and resuspension...

Efficient Recovery of Ultrapure Plasmid DNA

... Fig. 1: Restriction digestion of 40 individual plasmids with ecori (Roche Molecular Biochemicals). Vector pVL1392; length: 9.6 kb; bacteria strain: XL-1 Blue; medium 2x YT. The plasmid DN...
EcoRI in Biological Definition

Restriction enzyme

...strain) I First identified Order ID'd in bacterium Examples Enzyme Source Recognition Sequence Cut ecori Escherichia coli 5'GAATTC 5'---G AATTC---3' 3'CTTAAG 3'---CTTAA ...
EcoRI in Biological Dictionary

Restriction fragment

... Patterns: A Tool for Comparing Microbial Communities and Assessing Community Dynamics ... Terminal restriction fragment patterns ... A 1000 bp ecori restriction fragment contains a gene you are interested in. ... Draw a restriction map of the fragment and show the distances, in base pai...

Restriction enzymes

... Restriction enzymes were discovered 40 years ago during investigations into the ... Among the first restriction enzymes to be purified were ecori and EcoRII from ... Restriction endonucleases are enzymes that cleave the sugar-phosphate backbone of DNA. ... The presence of restriction e...
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(Date:4/18/2014)... Benaroya Research Institute at Virginia Mason (BRI) recently ... molecule in metastatic breast cancer reduces both the growth ... BRI scientists have found in models of the disease ... - 80 percent and can keep the tumor from ... five-year grant comes from the National Cancer Institute of ...
(Date:4/17/2014)... inflammation in benign prostate tissue was associated with ... was found even in those with low prostate-specific ... in Cancer Epidemiology, Biomarkers & Prevention , ... Research. , An analysis of prostate tissue biopsies ... of the Prostate Cancer Prevention Trial (PCPT) found ...
(Date:4/17/2014)... PLOS Pathogens , children who live in regions of ... immune response to infection with malaria parasites that may ... and illness and partially control the growth of malaria ... develop future interventions that prevent or mitigate the disease ... 200 million cases of malaria occur worldwide, resulting in ...
(Date:4/17/2014)... MD, was recently invited to submit a review ... Microbiology , one of the world,s foremost scientific ... specialist, and chair of the Department of Pediatrics ... analyzed the epidemiology and microbiology of co-infections during ... as more recent 2009 novel H1N1 pandemic. ...
(Date:4/17/2014)... The cause of neuronal death in Parkinson,s disease is ... may be mistaken for foreign invaders and killed by ... autoimmune diseases like type I diabetes, celiac disease, and ... published April 16, 2014, in Nature Communications . ... in Parkinson,s disease; but if true, it could lead ...
Breaking Medicine News(10 mins):Health News:Benaroya Research Institute evaluates immunology approach to blocking breast cancer 2Health News:Chronic inflammation may be linked to aggressive prostate cancer 2Health News:Chronic inflammation may be linked to aggressive prostate cancer 3Health News:Study sheds light on how the immune system protects children from malaria 2Health News:Is Parkinson's an autoimmune disease? 2
(Date:4/17/2014)... study suggests that fish consumption advisories for expecting ... long-lived contaminants like persistent organic pollutants (POPs). ... researchers including University of Toronto Scarborough PhD student ... how different levels of environmental contamination, a mother,s ... in the body influenced exposure in her children. ...
(Date:4/17/2014)... Kansas State University engineer has developed a patented ... improvised explosive devices. The same technique could help ... Steven M. and Kay L. Theede chair in ... engineering, and his research team have created a ... in car trunks. The distance detection method ...
(Date:4/17/2014)... and pathogens that devastate honeybees in Europe, Asia and ... do not appear to be impacting native honeybee populations ... researchers., The invasive pests include including Nosema microsporidia ... honeybees appear to be resilient to these invasive pests, ... control pests in Europe, Asia and the United States ...
Breaking Biology News(10 mins):Fish consumption advisories fail to cover all types of contaminants 2Patented research remotely detects nitrogen-rich explosives 2East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3
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