Navigation Links
EcoR in Biological Technology

siRNA Design Guidelines

...the 3' end of the oligonucleotide. In addition, for cloning into the pSilencer 1.0-U6 vector, nucleotide overhangs to the ecor I and Apa I restriction sites are added to the 5' and 3' end of the DNA oligonucleotides, respectively (Figure 2). In con...

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

...ncoding residues 208 to 313 of rat c-Fos 12 into the BamH I and ecor I sites of pFA-CMV. Our results ( figure 3A ) in tran...idues 1-96 of human ATF2 14,15 was inserted into the BamH I and ecor I sites of the pFA-CMV vector. Consistent with previous reports,...

New Competent Cells for Highest Transformation Efficiencies

...nd the potential size bias, two plasmid vectors were used. Stratagenes pcmv-script vector (4.2 kb) was digested with Xho I and ecor I; Stratagenes two-hybrid pAD-GAL4 vector (7.2 kb) was also digested with the same two enzymes. A mouse brain cDNA library was p...

Easy Cosmid DNA Purification with StrataPrep Kits

...absent. The resulting cosmid DNA was digested to completion with ecor I ( Figure 1 ). ...smid DNA is completely digested with restriction enzymes such as ecor I ( Figure 2 ). 1 Fig.2 ...

High-Titer Retroviral Vectors for Gene Delivery

...ermination Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the ecor I and BamH I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) w...

Generate High-Quality, Directional Plasmid cDNA Libraries

...using Pfu DNA polymerase, 4 ligated to ecor I adaptors and digested with Xho I. Since ...mpatible overhang on its 3 end and an ecor I-compatible overhang on the 5 end. A...4-2.1 vector predigested with Xho I and ecor I (XR) restriction enzymes. Transformation ...

Constructing Directional cDNA Libraries from Limited Amounts of RNA

...ific LIC-Right sequence. After standard second-strand synthesis, a double-stranded adaptor, the LIC-L-adaptor, which encodes the ecor I cohesive end and an LIC(L) sequence, is ligated to the double-stranded cDNA ends. The adaptor-ligated cDNA is then used as a t...

Versatile Reporter Vectors for Monitoring Viral Transduction

... seen for the humanized, red-shifted A. victoria GFP (EGFP) (data not shown). The coding sequence for hrGFP was inserted between the ecor I and Xho I sites in the pFB vector to produce the pFB-hrGFP vector (Figure 1A). The vector was then tested in retrov...

A New Lambda Vector for Mammalian Expression

...he Lambda ZAP-CMV vector predigested with either ecor I only or ecor I and Xho I, host strains, and helper phage. In ...e. We constructed a cDNA library in the ecor I and Xho I sites of the Lambda ZAP-CMV vector ...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...-selected cDNA was inserted between the ecor I and Xho I sites of the plasmid pFB ( ...assess the quality of both the phosphatase-treated ecor I/Xho I-digested vector as well as the ...zed. All of the 100 clones examined had ecor I/Xho I-excisable inserts, with an aver...

More>>

EcoR in Biological Dictionary

Restriction enzymes

...riction enzymes were discovered about 30 years ago during investigations ... Among the first of these " restriction enzymes " to be purified were ecor I and ... In fact, more than 900 restriction enzymes , some sequence specific and some not, ... These restriction enzymes generally have n...

Restriction

...striction enzymes were discovered about 30 years ago during investigations ... Among the first of these " restriction enzymes" to be purified were ecor I and ... ... information on intrauterine growth restriction , including causes, diagnosis, treatment, management, ... Intrauterine growth restri...
Other Tags
(Date:4/17/2014)... many as 10 million older Americans suffer from depression, ... , However, new research a project that followed ... six years found that Internet use among the ... than 30 percent. , "That,s a very strong effect," ... telecommunication, information studies and media who led the project. ...
(Date:4/17/2014)... CHICAGO, IL, April 17, 2014-- Colic affects about one ... accounts for numerous pediatric visits during the first several ... of colic symptoms was showing promise; however, the April ... (BMJ2014;348:g2107; Sung, Valerie) reported on a study, "Probiotics ... probiotic L reuteri for infant colic ...
(Date:4/17/2014)... from Boston University School of Medicine (BUSM) have ... consumption in an experimental model. The findings, reported ... Alcohol Abuse , may lead to more effective ... is one of the leading causes of illness ... negative economic impact by limiting the productivity of ...
(Date:4/17/2014)... An international team led by researchers at UC Davis ... plays a key role in cell division, also boosts ... the first time the complex has been shown to ... B1/Cdk1 an excellent target to control cellular energy production, ... was published online today in the journal Developmental ...
(Date:4/17/2014)... Cesar A. Arias, M.D., Ph.D., at The University of ... a new superbug that caused a bloodstream infection in ... 17 issue of The New England Journal of ... a class of highly-resistant bacteria known as methicillin-resistant ... cause of hospital and community-associated infections. The superbug has ...
Breaking Medicine News(10 mins):Health News:Internet use can help ward off depression among elderly 2Health News:New study says probiotic use for infant colic is not effective in reducing symptoms 2Health News:BUSM researchers find anti-seizure drug may reduce alcohol consumption 2Health News:Dual role: Key cell division proteins also power up mitochondria 2Health News:New MRSA superbug emerges in Brazil 2
(Date:4/17/2014)... need to produce hydrogen sulfide in order to properly ... study from the Center for Craniofacial Molecular Biology at ... Professor Songtao Shi, principal investigator on the project, said ... governs the flow of calcium ions. The essential ions ... osteogenesis, or the creation of new bone tissue, and ...
(Date:4/17/2014)... in German . ... money demanded of him, he can expect his premises to ... fear of the consequences is enough to make restaurant owners ... which lay their eggs in other birds, nests. If the ... take their revenge by destroying the entire nest. Consequently, it ...
(Date:4/17/2014)... in France have hit on a novel method ... the correct and most effective treatment possible., Kidney ... western and developing world. If left untreated, apart ... renal failure and other complications. In many patients ... Clearly a more effective pathological approach to diagnosis ...
Breaking Biology News(10 mins):Fear of the cuckoo mafia 2Fear of the cuckoo mafia 3
Other Contents