Breakthrough in cell-type analysis offers new ways to study development and disease

...When one of these modified BACs is transferred into mice, expression of the egfp mimics that of the gene of interest, lighting up cells with a green glow th...P. Heintz, postdoctoral fellow Myriam Heiman and colleagues attached an egfp to the surface of the ribosome and used it as a handle to pick out the cell...

EGFP in Biological News

Studies on imaging and tracking transplanted cells

... lead author Dr. Techung Lee of the SUNY Buffalo Department of Biochemistry. The research team experienced 'inconsistency' when attempting to track egfp and LacZ-labeled MSCs while the DAPI-labeled cells could be 'stably retained' in the implanted MSCs for at least one month after implantation. "These...

Gold nanoparticles could improve antisense cancer drugs

...luorescent protein (EGFP) expressed in a mouse cell. The antisense sequence of the DNA attached to the nanoparticles was complementary to the mRNA for egfp expression. When the nanoparticles were introduced to the cells the fluorescence dimmed -- a result of the nanoparticles binding to the mRNA and shutt...

EGFP in Biological Technology

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...n is described (EGFP; Bhattacherjee et al., 2004). egfp is a recombinant protein originally derived from t... requiring cell/tissue fixation for visualization. egfp fluoresces green when excited by light of 488 nm a... that are not deleterious to living tissues. Since egfp consists of ~200 amino acid residues, it may be ex...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

...adenoviral vector • viral transduction • egfp • IN Cell Analyzer • gene delivery ...s application note describes the performance of an egfp reporter assay for assessing the translocation of ...itoring the cellular position and intensity of the egfp fluorescent signal from the chimera gene product, ...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

...adenoviral vector • viral transduction • egfp • IN Cell Analyzer • gene delivery ... >This application note presents data from an egfp translocation assay focusing on the glucocorticoid...ne and prednisolone.

Quantitation of the egfp signal from the EGFP-GCCR fusion protein within th...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

...ition via dynamic expression and degradation of an egfp sensor that shadows endogenous cyclin B1 levels. T...hase to S phase transition via translocation of an egfp fusion protein incorporating the phosphorylation-d...nces present only in endogenous cyclin B1 retained egfp expression and their fluorescence increased indica...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

... protein, as a reporter gene in Jurkat cells. egfp has an excitation maximum at 488 nm, an....5. 3 In fluorometric assays, purified egfp can be measured at concentrations in th...ecent experiments we have shown that intracellular egfp can be assayed using a VersaFluor fluor...

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

...s, live cells in the CBNF-positive population were cross-gated onto the egfp histogram. A parallel measurement of the same cell samples on a flow ...ncreased to 8. It indicated that optimal transfection and expression of egfp were achieved when a DNA:lipid ratio of 6 or 8 was used. A comparable r...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

...on day 9 in vitro using various ratios of egfp plasmid DNA (carrying a CMV promoter and isolated ...tro, cultures were treated with 200 ng of egfp plasmid DNA complexed with 5 l of Effecte...re stained using DAPI (blue). C Cells expressing egfp appear green. D Superimposed images. ...

L2

... dish of a 6-well plate. Detection methods for transfection: The expression of egfp driven by the insect actin promotor can be detected clearly after 24-48 hours by FACS analysis or under a fluorescence microscope. ...

Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

...ems, they compared two liposome-based formulations, LipofectAMINE and LipoTAXI transfection reagents. 4 The pEGFP-C3 plasmid, which encodes the egfp with expression driven by the CMV promoter, and either LipofectAMINE or LipoTAXI reagent were used to transfect SW13 cells. The cells (8 x 10 4 ) ...