Navigation Links
EGFP in Medical News

Breakthrough in cell-type analysis offers new ways to study development and disease

...When one of these modified BACs is transferred into mice, expression of the egfp mimics that of the gene of interest, lighting up cells with a green glow th...P. Heintz, postdoctoral fellow Myriam Heiman and colleagues attached an egfp to the surface of the ribosome and used it as a handle to pick out the cell...
EGFP in Biological News

Studies on imaging and tracking transplanted cells

... lead author Dr. Techung Lee of the SUNY Buffalo Department of Biochemistry. The research team experienced 'inconsistency' when attempting to track egfp and LacZ-labeled MSCs while the DAPI-labeled cells could be 'stably retained' in the implanted MSCs for at least one month after implantation. "These...

Gold nanoparticles could improve antisense cancer drugs

...luorescent protein (EGFP) expressed in a mouse cell. The antisense sequence of the DNA attached to the nanoparticles was complementary to the mRNA for egfp expression. When the nanoparticles were introduced to the cells the fluorescence dimmed -- a result of the nanoparticles binding to the mRNA and shutt...
EGFP in Biological Technology

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...n is described (EGFP; Bhattacherjee et al., 2004). egfp is a recombinant protein originally derived from t... requiring cell/tissue fixation for visualization. egfp fluoresces green when excited by light of 488 nm a... that are not deleterious to living tissues. Since egfp consists of ~200 amino acid residues, it may be ex...

Performance of Ad-A-Gene EGFP-MAPKAP-k2: an adenoviral vector gene delivery system

...adenoviral vector • viral transduction • egfp • IN Cell Analyzer • gene delivery ...s application note describes the performance of an egfp reporter assay for assessing the translocation of ...itoring the cellular position and intensity of the egfp fluorescent signal from the chimera gene product, ...

Performance of Ad-A-Gene EGFP-Glucocorticoid Receptor (EGFP-GCCR): an adenoviral vector gene delivery system

...adenoviral vector • viral transduction • egfp • IN Cell Analyzer • gene delivery ... >This application note presents data from an egfp translocation assay focusing on the glucocorticoid...ne and prednisolone.

Quantitation of the egfp signal from the EGFP-GCCR fusion protein within th...

siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

...ition via dynamic expression and degradation of an egfp sensor that shadows endogenous cyclin B1 levels. T...hase to S phase transition via translocation of an egfp fusion protein incorporating the phosphorylation-d...nces present only in endogenous cyclin B1 retained egfp expression and their fluorescence increased indica...

Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer

... protein, as a reporter gene in Jurkat cells. egfp has an excitation maximum at 488 nm, an....5. 3 In fluorometric assays, purified egfp can be measured at concentrations in th...ecent experiments we have shown that intracellular egfp can be assayed using a VersaFluor fluor...

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

...s, live cells in the CBNF-positive population were cross-gated onto the egfp histogram. A parallel measurement of the same cell samples on a flow ...ncreased to 8. It indicated that optimal transfection and expression of egfp were achieved when a DNA:lipid ratio of 6 or 8 was used. A comparable r...

Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures

...on day 9 in vitro using various ratios of egfp plasmid DNA (carrying a CMV promoter and isolated ...tro, cultures were treated with 200 ng of egfp plasmid DNA complexed with 5 l of Effecte...re stained using DAPI (blue). C Cells expressing egfp appear green. D Superimposed images. ...

L2

... dish of a 6-well plate. Detection methods for transfection: The expression of egfp driven by the insect actin promotor can be detected clearly after 24-48 hours by FACS analysis or under a fluorescence microscope. ...

Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance

...ems, they compared two liposome-based formulations, LipofectAMINE and LipoTAXI transfection reagents. 4 The pEGFP-C3 plasmid, which encodes the egfp with expression driven by the CMV promoter, and either LipofectAMINE or LipoTAXI reagent were used to transfect SW13 cells. The cells (8 x 10 4 ) ...
Other Tags
(Date:4/20/2015)... Using Quality by Design to Improve Manufacturing: ... Compliance , **FDAnews Webinar**, May 7, 2015 — 1:30 ... The life sciences industries are going through enormous financial ... less. Mergers, acquisitions, the patent cliff and more ... speed up production while still maintaining quality and compliance. ...
(Date:4/20/2015)... 21, 2015 The National Association ... Founder of Soul Care Group, Inc., as a ... recognized with this prestigious distinction for leadership in business. ... Chapters, NAPW is the nation's leading networking organizaiton exclusively ... with our Woman of the Year Award,” says NAPW ...
(Date:4/20/2015)... 20, 2015 The partners of Urgent Care ... to Bella Diosa Laser Med Spa with a special event. ... party on from 12–7 p.m. on April 24 on 909 ... drinks and refreshments. Participants will also get the chance to ... free gift with any purchase, as well as up to ...
(Date:4/20/2015)... Mauritius is a seemingly idyllic and tranquil island oasis ... miles) off the southeastern coast of Africa, the tiny ... where everyone lives together in harmony. , But the ... with drug abuse. In fact, it carries a dubious ... nation in eastern Africa. , “Many youngsters, they know ...
(Date:4/20/2015)... 2015 At the famous Avalon Hollywood ... Movie Awards” EcoLuxe Lounge, with Choices Recovery as the ... of sustainable product placement in TV and film, gathered ... and ecologically sound goods and services for this daylong ... hosted the Choices Celebrity Interview Lounge, speaking with many ...
Breaking Medicine News(10 mins):Health News:FDAnews Announces — Using Quality by Design to Improve Manufacturing: How Sponsors and CMOs Speed Production and Improve Compliance Webinar, May 7, 2015 2Health News:FDAnews Announces — Using Quality by Design to Improve Manufacturing: How Sponsors and CMOs Speed Production and Improve Compliance Webinar, May 7, 2015 3Health News:National Association of Professional Women Announces Susan Binau, Founder of Soul Care Group, Inc., a 2015 Professional Woman of the Year 2Health News:Bella Diosa Says Hello to Yonkers Community with Spa Party 2Health News:Voice For Humanity: Choolun Bhojoo leads the fight to rid Mauritius of a drug plague 2Health News:Choices Recovery South Bend Talks About Healthy Decisions with TV's Adrienne Janic 2
(Date:3/31/2015)...  Elephant Talk Communications Corp. (NYSE MKT: ETAK) ("Elephant ... Defined Network Architecture (ET Software DNA® 2.0) platforms and ... $20.4 million for the year ended December 31, 2014 ... year ended December 31, 2013. At ... its multi-year transition away from its legacy landline business ...
(Date:3/24/2015)... Markets ( http://www.researchandmarkets.com/research/kvdtcw/face_and_voice ) has announced the addition of ... report to their offering. This report analyzes the ... the following Segments: Face Biometrics, and Voice Biometrics. The report provides ... Japan , Europe , ... Africa , and Latin America ...
(Date:3/23/2015)... ANGELES , March 23, 2015  In the ... Federal fraud case conviction losses. This figure accounts for ... which, according to The Nilson Report, exceeds $11 billion ... investigative tool after a crime has occurred. Investor ... and polygraph expert Joe Paolella partnered to ...
Breaking Biology News(10 mins):Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 2Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 3Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 4Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 5Face and Voice Biometrics - Global Strategic Business Report 2015: Face Recognition Gains Prominence in Immigration Control 6Polygraph Test Now Used to Protect Investors From Fraud 2Polygraph Test Now Used to Protect Investors From Fraud 3
Other Contents