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Dulbeccos in Biological Technology

Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)

...tains luciferin (lyophilized) and luciferase assay buffer. 4. PBS 1X - dulbeccos Phosphate Buffered Saline (PBS). Irvine Scientific, Cat. #9235. Phone: 800-... 1. PBS-BSA solution was prepared by adding 1% bovine serum albumin to dulbeccos PBS 1X. 2. The firefly luciferase stock standard (1 mg/mL) was pre...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

...the LipofectAMINE:DNA complex, and added to cells prewashed with dulbeccos MEM to remove serum and antibiotics. The transfection continued ...ime the LipofectAMINE:DNA complex was replaced with high-glucose dulbeccos MEM containing 5% fetal bovine serum, penicillin and streptomyci...

Rapid hit confirmation and early hERG liability testing using the IonWorks HT system

... Fisher Cat. #SS266-1) Tissue culture flasks: Cells grown in T-75 flasks (Corning Cat. #430641) Cell culture media: dulbeccos Modified Eagle Medium (Gibco, Cat. #11965-092) 10% Fetal Bovine Serum (FBS, Irvine Cat. #3000), 1% Penicillin-Streptomycin ...

The QIAGEN Guide to Animal Cell Culture

...itable medium for each cell type must be determined experimentally. Common basal media include Eagle minimal essential medium (MEM), dulbeccos modified Eagle medium (DMEM), RPMI 1640, and Ham F10. These contain a mixture of amino acids, glucose, salts, vitamins, and other nu...

Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators

... than the control, when cells were treated with 100 ng/ml of EGF, and almost 50-fold higher when cells were challenged with PMA (60 ng/ml). dulbeccos modified Eagles medium (DMEM) and dimethylsulfoxide (DMSO) were used as negative controls. Fig.4 F...

Optimizing Transfection Conditions for Studying Signal Transduction Pathways

...nsfection was kept constant with different amounts of an unrelated plasmid (pBluescript plasmid). After transfecting, cells were maintained in dulbeccos minimal essential medium containing 0.5% fetal bovine serum. The pFR-Luc reporter plasmid, which contains the entire coding sequence of the lucife...
Dulbeccos in Biological Products
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