Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)
...tains luciferin (lyophilized) and luciferase assay buffer.
4. PBS 1X - dulbeccos
Phosphate Buffered Saline (PBS). Irvine Scientific, Cat. #9235. Phone: 800-...
1. PBS-BSA solution was prepared by adding 1% bovine serum albumin to dulbeccos
2. The firefly luciferase stock standard (1 mg/mL) was pre...
Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
...the LipofectAMINE:DNA complex, and added to cells prewashed
MEM to remove serum and antibiotics. The transfection
continued ...ime the LipofectAMINE:DNA complex was replaced
with high-glucose dulbeccos
MEM containing 5% fetal bovine serum, penicillin
Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
... Fisher Cat. #SS266-1)
Tissue culture flasks: Cells grown in T-75 flasks (Corning Cat.
Cell culture media: dulbeccos
Modified Eagle Medium (Gibco,
10% Fetal Bovine Serum (FBS, Irvine Cat. #3000), 1% Penicillin-Streptomycin
The QIAGEN Guide to Animal Cell Culture
...itable medium for each
cell type must be determined experimentally. Common basal media include
Eagle minimal essential medium (MEM), dulbeccos
modified Eagle medium
(DMEM), RPMI 1640, and Ham F10. These contain a mixture of amino acids,
glucose, salts, vitamins, and other nu...
Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion
... than the control, when cells were treated with 100 ng/ml of EGF, and almost
50-fold higher when cells were challenged with PMA (60 ng/ml). dulbeccos
modified Eagles medium (DMEM) and dimethylsulfoxide (DMSO) were
used as negative controls.
Optimizing Transfection Conditions for Studying Signal Transduction Pathways
...nsfection was kept constant with different amounts of an
unrelated plasmid (pBluescript plasmid).
After transfecting, cells were maintained in dulbeccos
medium containing 0.5% fetal bovine serum. The pFR-Luc reporter plasmid, which
contains the entire coding sequence of the lucife...