Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS
Trypsin, sequencing grade 17-6002-75
PlusOne Tris 17-1321-01
The Bio-Rad Silver Stain, Rev E
step, gels are primed with a reducing agent such as dithiothreitol
an oxidizing reagent such as permanganate or dichromate. Dich...e images obtained have higher sensitivity and
lowest background. dithiothreitol
generates signals of lower sensitivity,
while permanganate gener...
Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B
Following ammonium sulfate precipitation and ion exchange chromatography,
size exclusion column fractions, in 10 mM KPO 4 , 2 mM dithiothreitol
0.1 mM NADP+ (pH 6.2), possessing NADP-GDH activity were pooled and concentrated
via Diaflo filtration from 17.5 ml to 5 ...
Titan One Tube RT-PCR System / Kit
...Taq/Pwo enzyme blend, for PCR)
Additional reaction components (RT-PCR reaction buffer with 7.5 mM
MgCl 2 ; MgCl 2 solution; dithiothreitol
for convenient, quick reaction set-up
In addition to all the components of the Titan One Tube RT-PCR System...