Genome Wide Profiling of Paired Cancerous and Normal Breast Tissues and Rapid Interpretation of Gene Expression Data
...r) using two replicates for each sample (Figure 1). Total RNA samples (from Biochain Inc., Hayward, CA) obtained from two patients were processed into digoxigenin
(DIG) -labeled cRNA using the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit.
The labeled DIG-cRNA (10 μg per microarray) was...
Tools for Proteomic Science II
... of proteins with
alkaline phoshatase, biotin, digoxigenin, fluorescein, or
peroxidase. Streptavidin and antibody conjugates recognize
biotin or digoxigenin
and fluorescein, and allow the
convenient detection of labeled proteins.
Introducing molecular tags, such as digoxigenin
(DIG) or biotin-labeled dUTP into the PCR product, as it was amplified
made PCR an invaluable tool for medical diagn...
Digoxigenin in Biological Products
Nuclear Matrix Protein ELISA Kit from Calbiochem
Description:...ction (Nuclear Matrix Protein) ELISA allows quantitation of cell death. Kit Contents: Coated 96-well removable strip plate, lyophilized NMP standard, digoxigenin
conjugated detector antibody, HRP conjugated anti-digoxigenin secondary antibody, substrate, sample diluent, 20X plate wash concentrate, stop solution...
Digoxigenin in Biological Dictionary
...pDNA . ... The clones from the cpDNA library constructed from Nicotiana tabacum (9) were used as probes. The cpDNA probes were labeled with digoxigenin
dUTP by random priming. ... Chloroplast DNA ( cpDNA ) sequence comparisons have been a widely used tool in ... Second, cpDNA intergenic regio...