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Dideoxy in Biological News

First individual genome sequence published

...f constructing a true reference human genome based on one individual. Using whole genome shotgun sequencing and highly accurate long reads from Sanger dideoxy automated DNA sequencing, the team produced additional data making the final 32 million sequences. From the combined data set of more than 20 bill...
Dideoxy in Biological Technology

Preventing Carry-over Contamination with Uracil-DNA Glycosylase

... PCR products containing dU perform as well as those containing dT when used as hybridization targets or as templates for dideoxy sequencing. PCR products containing dU can be cloned directly, if they are transformed into ...

Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

...y members, ran the PCR products in agarose gel electrophoresis, and purified the PCR fragments (QIAGEN Inc). Sequencing was performed with fluorescent dideoxy dye-primer on an ABI Prism 377 sequencer, and all results were visually inspected. REFERENCES Cargill, C., et al. (1999) N...

Automated Fluorescent-Tag Cycle Sequencing

...not produce equal cycling conditions in each well may not incorporate the dideoxy nucleotide reaction terminators at a uniform rate between sample wells. I...showing the relative fluorescent intensities of each of the four dideoxy nucleotide reaction terminators (A-Dye Terminator, C-Dye Termina...
Dideoxy in Biological Products

SNP Scoring Service from Polymorphic DNA Technologies, Inc.

Description:... Accurate To score SNPs, Polymorphic uses proven, validated Sanger dideoxy DNA sequencing, the gold standard in DNA analysis. We use high throughput capillary sequencing instrumentation. The polymorphic locus can be confirmed...
Company:Polymorphic DNA Technologies, Inc.

SNP Discovery Service from Polymorphic DNA Technologies, Inc.

Description:...imers, PCR amplification, DNA sequencing and data analysis. Accurate For SNP discovery, Polymorphic uses the gold standard in DNA analysis, Sanger dideoxy DNA sequencing. We use high throughput capillary sequencing instrumentation. To ensure accuracy and sensitivity, only data from the high resolution, h...
Company:Polymorphic DNA Technologies, Inc.

Exo- Klenow Fragment (Cloned) from Ambion

Description:...- Klenow Fragment the enzyme of choice for isotopic or biotin labeling of DNA probes by the random primed method, and for DNA sequencing by the Sanger dideoxy method. Quality Control The Exo- Klenow Fragment is thoroughly tested for nonspecific contaminating endonuclease, exonuclease and protease activity....
Company:Ambion
Dideoxy in Biological Definition

Primer

...hemselves, but order them by specialized companies. DNA sequencing is used to determine the nucleotides in a DNA strand. A sequencing method called dideoxy sequencing (also known as chain termination method or Sanger method ) uses a primer as a start marker for the chain reaction. In polymerase chai...
Dideoxy in Biological Dictionary

Polymorphic loci

...to score SNPs. The polymorphic locus is highlighted in ... putting SNP ... Accurate. To score SNPs, Polymorphic uses proven, validated Sanger dideoxy ... The polymorphic locus can be confirmed by sequencing in ... The overall mean proportion of polymorphic loci was 61.19 percent, and th...

DNA sequencing

...me, thereby promising to advance knowledge of human biology and improve medicine. This ... Of the two major methods for sequencing DNA , the dideoxy chain ... The enzyme used in manual DNA sequencing was a modified version of the phage T7 ... 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14...

Dideoxy Sequencing

...e chain, no further nucleotides can be... The dideoxy method gets its name from the critical role played by synthetic ... For this reason, the dideoxy method is also called the chain termination metho...f reagents and reagent systems for the ... Sanger dideoxy terminator sequencing , the ... ...
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