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Deproteinization in Biological Technology

InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C

Prepare DNA from blood, cultured cells, or bacteria: Just incubate sample with InstaGene matrix at 56C for 1530 min, then boil for 8 min. InstaGene matrix absorbs cell lysis products, producing an improved substrate for PCR amplif...

Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)

...tubes (12 x 75 mm, glass), or 1 cm cuvettes, or transparent microplates (e.g., polystyrene) 3. Trichloroacetic acid, (20% w/v) if samples need deproteinization 4. Pipettor and tips 5. Inorganic Phosphorus kit (Sigma Chemical Co., catalog # 670-C) SIGMA KIT METHODS Setting up th...

Nuclease Protection Assays: The Basics

...brane disruption Inhibition of ribonuclease activity deproteinization Recovery of intact RNA Ambion provides several options for ...

Northern Analysis: The Basics

...brane disruption Inhibition of ribonuclease activity deproteinization Recovery of intact RNA Ambion provides several options for ...

Genomic DNA Isolation Protocol(1,2,3)

...NA is obtained from freshly prepared nuclei. Proceed to Section II. II Lysis of nuclei and deproteinization of DNA Suspend pelleted nuclei in 2 ml Saline/EDTA Solution (75 mM NaCl, 24 mM EDTA) by repeated up and down pipe...

Genomic DNA Isolation Protocol(1,2,3)

...NA is obtained from freshly prepared nuclei. Proceed to Section II. II Lysis of nuclei and deproteinization of DNA Suspend pelleted nuclei in 2 ml Saline/EDTA Solution (75 mM NaCl, 24 mM EDTA) by repeated up and down pipe...
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