GenCatch Viral RNA Mini-Prep Kit
Description:... nucleic acids from cell-free samples. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature
proteins. Purified viral RNA is suitable fro applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of ...
Company:Epoch Biolabs, Inc
Denature in Medical Definition
Artificial induction of immunity
...h less than is required by the immune system to recognize the toxin and produce antibodies against it. However, heating the tetanus toxin enough to denature
it causes it to lose its ability to produce disease, but still leaves it able to induce immunity to tetanus when injected into subjects. The heated, ...
Denature in Biological News
Denature in Biological Technology
Probiotic Delivery Technology Awarded Third International Patent
... organisms through the
-- Survival on the store shelf
-- Safe passage through the harsh stomach acids that may denature
BIO-tract products incorporate proven processes and technologies to
overcome these barriers.
How BIO-tract wor...
Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000
...tion A1 is a tipbox, A2 is tip disposal, B2 is the denature
buffer reservoir, C2 is the TempliPhi reaction pla...pe of PCR wells, when dispensing the 10 μL of denature
buffer, we dispensed 5 μL of the volume very ... Reservoir: The type of reservoir for holding denature
buffer simply needs to be sufficient to hold the r...
GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
>The mixture was briefly centrifuged and then incubated at 30 ºC for 16-18 h. The reaction was stopped by heating at 65 ºC for 10 min to denature
the Phi29 enzyme. At this point, the reactions can be kept on ice or stored at -20 ºC until needed. Note that reaction volumes can be increased o...
Oragene and Whole Genome Amplification with GenomiPhi
...ns provided with the GenomiPhi kit, 1 L of DNA was
added to 9 L of sample buffer containing random hexamers and heated to
95C to denature
the DNA. The sample was cooled and mixed with 9 L of
reaction buffer containing salts and deoxynucleotides and 1 L of enzyme
Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
...s and 72 C for 60 s. A final elongation step
of 8 minutes at 72 C was then carried out. The temperature cycler was
programmed to denature
the amplification products at 96 C for 8 minutes
and to then perform slow cooling to 40 C. Without this last step, heteroduplex
Detection of Mutations in the CYP21 Gene Using the DCode System
... The mutant PCR product was mixed with
the corresponding wild type PCR product; the mixture was placed at 96
C for 10 minutes to denature
the PCR products and subsequently left at
room temperature for gradual renaturation, thereby generating heteroduplex
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
...bility is decreased as
DNA denaturation begins. Mutant and wild-type molecules have different
melting behavior and thus begin to denature
at different points in time
resulting in a separation on the gel.
The DCode system performs TTGE by controlling the bu...
Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A
different melting behavior. Since the T m values were different for each
the PCR products (see T m values, Table 1), they should denature
points along the gel, resulting in separate bands on the TTGE gel even
though the PCR products were similar in mole...
Acrylamide Polymerization A Practical Approach
...flavin may cause the oxidation of some
compounds, especially sulfhydryl-containing compounds
(Dirksen and Chrambach 1972), and can denature
(Righetti et al. 1981).
Reducing the concentration of initiators results in longer
polymer chain lengths, lower ...
The World's Best DNase Improved TURBO DNA-free
...ce RNA hydrolysis when
the sample is heated. This is particularly important for
researchers who need to heat denature
RNA samples prior to
Maximum RT-PCR Sensitivity
The extraordinary linea...
Denature in Biological Definition
...aller molecules is the need for assistance in dissolving into solution. Many require salts or particular ions to dissolve in water. Proteins will denature
if the solute concentration of their solution is too high or too low.
According to IUPAC recommendations the term macromolecule is reserved for a...
Denature in Biological Dictionary
Denatured proteins can exhibit a wide range of characteristics, from loss of ... A classic example of denaturing in proteins comes from egg whites, which are ... Denaturation. From Wikipedia, the free encyclopedia (Redirected from Denature
) ... Denatured alcohol, also known as m...
...s using various types of gel. ... In SDS -polyacrylamide gel electrophoresis ( SDS - PAGE ), proteins are separated ... SDS does however denature
the protein, so activity stains cannot be used to ... SDS - PAGE . Two-dimensional gel electrophoresis. Temperature gradient gel electrophor...
...r instance, gels used in SDS-PAGE will unfold and denature
the native structure of a protein. In contrast to...ing electrophoresisis desirable since it helps to denature
the ... ... denaturing acrylamide gel of 2...ingelectrophoresis is desirable since it helps to denature
the sample, ... DGGE Denaturing gradient Ge...