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Denaturation in Medical Products

ThermoBrite

Description:... This programmable system automates the denaturation and hybridization steps in slide-based (F)ISH procedures, and provides walk-away convenience for clinical and research personnel. The low cost unit ac...
Company:Iris Sample Processing (formerly StatSpin, Inc.)

TPO (Ab) RIA Thyroid Function 014-HD-90.1 Thyroid Peroxidase

Description:...tructure of the TPO. The anti- gen TPO used in this competitive assay is indirectly labelled with a monoclonal iodine-125 antibody to prevent the denaturation of the sensitive antigen. In connection with the monoclonal antibody on the solid phase this method guarantees a high sensitivity and specifi...
Company:ALPCO Diagnostics

Vysis HYBrite

Features:... Eliminates the need for separate sample denaturation - probe and target DNA are co-denatured together Supports a variety of specimen types - assay parameters are supplied for Vysis CEP, LSI and WCP probe...
Company:Abbott Diagnostics
Denaturation in Medical Dictionary

Detergents

...nt Chemistry ... Detergents seem to be a necessary evil for the protein chemist. ... However, the presence of detergents can also result in denaturation of proteins ... Detergents , term applied to materials, the solutions of which aid in the removal ... Detergent - Wikipedia, the free encyclope...
Denaturation in Biological Technology

Noridian Medicare Now Covers Renessa(R) Treatment for Incontinence in Women

...eling of the female bladder neck and proximal urethra for stress urinary incontinence. This includes the Renessa transurethral collagen radiofrequency denaturation procedure." The website goes on to state that "coverage will be allowed when the procedure is performed consistent with FDA labeling and in accordanc...

QIAGEN Fast Cycling PCR Kit

...(Figure 4). The unique buffer formulation and optimized DNA polymerase concentration also enables a significant reduction in the denaturation and extension times. Fast Cycling PCR Buffer has been developed to eliminate the need for optimi...

REPLI-g UltraFast Mini Kit

... methods. In addition, a unique alkaline denaturation buffer guarantees gentle denaturation of genomic DNA and avoids fragmentation of template DNA caused by heat denaturation methods. This ensures more sensitive and ...

Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers

... The SSCP technique includes three processes: PCR amplification using primers that flank the DNA region of interest; denaturation of the resulting double-stranded PCR product, followed by rapid chilling to prevent re-annealing of the strands; Electrophoretic separatio...

Quantitative Analysis of the Cell Cycle in Imaging Applications

...ynkiewicz, L. Staiano-Coico and M. R. Melamed. 1985. Detection of 5-bromodeoxyuridine (BrdUrd) incorporation by monoclonal antibodies: role of the DNA denaturation step. J. Histochem. Cytochem. 33:821-827. 6. Shapiro, H. M., Practical Flow Cytometry, 3rd Edition, Wiley-Liss, New York. Vanderlaan, M. ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...enomiPhi DNA Amplification Kit After DNA isolation, 10 ng was amplified using GenomiPhi DNA Amplification Kit. Amplification without a heat denaturation step (formalin fixation also contributes to DNA denaturation) was completed over 18 h at 30 °C, followed by enzyme inactivation by heating at 65 &...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...ed fragment migrates more slowly through a polyacrylamide gel than does an undegraded, helical, doublestranded DNA molecule. The denaturation reaction can be controlled by establishing a gradient of DNA denaturants such as urea and formamide within the gel. During elect...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...ife Science Inc., Cleveland, OH), 15 pmol of each primer and a minimum of 200 ng of DNA in a total volume of 50 l. After initial denaturation at 96 C for 1 minute, PCR* was carried out for a total of 33 cycles at an annealing temperature of 62 C (apoA-I) or 58 C (apoB),...

Real-Time PCR: General Considerations, Rev A

...o 65C) and time (dependent on primer T m and chemistry) Extension time (dependent on chemistry and product length) denaturation temperature and time (dependent on target sequence) 2-step v. 3-step PCR Experimental Design and Interpretation of R...

The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B

...loped to be used with the Bio-Rad iCycler iQ system. The gradient allows evaluation of up to eight annealing, polymerization, or denaturation temperatures in a single experiment. Temperature optimization can be accomplished using SYBR Green I or fluorescently labeled specific ...
Denaturation in Biological Products

SpotLight CISH Hybridizer (120 VAC, 50-60 Hz) from Invitrogen

Description:...ications(L x W x H): 17 3/4" x 8 15/16" x 5 5/16"18.7 lbsCapacity: 12 slidesTime: Programmable 0 - 100 hoursControl: Programmable 30 - 99C Automated denaturation and hybridization steps 40 user programmable protocols 3 operating modes (Denaturation/Hybridization, Hybridization only, and Fixed temperature) Rapid...
Company:Invitrogen

XpressPack™ Luminescent Amplification Detection System, required for luminescent XpressPack™ from CHEMICON

Description:...ing a microplate luminometer; the intensity of the light is directly proportional to the amount of amplicon present in the sample. Components: denaturation Solution Hybridization Buffer AquaLite® Wash Buffer, 10X AquaLite® Streptavidin Assay Buffer, 10X AquaLite® Reconstitution Buffer Aqu...
Company:CHEMICON

XpressPack™ Colorimetric Amplification Detection System, required for colorimetric XpressPack™ from CHEMICON

Description:...ed using a microplate reader; the intensity of the color is directly proportional to the amount of amplicon present in the sample. Components: denaturation Solution Hybridization Buffer Wash Buffer, 10X Strep:HRP Conjugate - Ready to Use Substrate (TMB/E) Stop Solution Applications: DNA Amplificatio...
Company:CHEMICON

ThermoBrite (120 VAC) from StatSpin

Description:... This programmable system automates the denaturation and hybridization steps in slide-based (F)ISH procedures, and provides walk-away convenience for clinical and research personnel. The low cost unit ac...
Company:StatSpin

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:...Specific PCR (MSP) followed by methylation analysis using restriction endonucleases, sequencing, microarrays, etc. SPECIFICATIONS *Format: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Column. *Recovery Volume: Purified, bisulfite treated DNA is recovered in less than or equal ...
Company:Zymo Research
Denaturation in Biological Definition

Denaturation

Denaturation in Biochemistry refers to a structural change in macromolecules caused by extreme conditions. Denaturation can refer to the intentional adulteration of ethyl alcohol so that it is unsuitable as a beverage. Denaturation of fissile materials is the process of...
Denaturation in Biological Dictionary

Denaturation

Denaturation is a process in which proteins or nucleic acids lose their tertiary and secondary structure by application of some external stress or compound, such as a strong acid or base, a concentrated inorganic salt, an... Denaturation (biochemistry), a structural change in mac...

Ribonuclease

...alyzes cleavage of the phosphodiester bond ... Ribonuclease A has a molecular weight of 13,700 daltons. ... Ahmad, F.: Free Energy Changes in denaturation of Ribonuclease A by Mixed Denaturants. ... Function of Pancreatic Ribonuclease , Nature 221, 340, 1969 ... Online Shopping at SpotCost.com - N...

Mispairing

... Mutation process which occurs during DNA replication. It involves denaturation and displacement of the DNA strands, resulting in mispairing of the complementary bases. Slipped strand mispairing is one explanation for the ...

Lysis

...Online.org dictionary. ... Cell lysis and Lowry assay for 2D electrophoresis ... As soon as lysis occurs, proteolysis, dephosphorylation and denaturation begin to occur. ... 20 min is a traditional interval for cell lysis . ... Online Shopping at DirectTextBook.com - Save up to 80%, buy or rent. ...

Denature

...ial to (fissionable material) so as to make ... denaturation – Encyclopedia.com has denaturation articles, denaturation pictures, video and information ... Factors other ...

Cooperativity

...operativity depends on changes in dimer–dimer ... cooperativity , but discovered that upon ... Most importantly, however, cooperativity of DNA denaturation causes this ... We also demonstrate the extent of cooperativity , and find that the melting map ... ...
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