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DTT in Biological News

Biophysical Society announces 2008 International Travel Awards

...d on Oxidative Stress and Induction of DNA Damage. Rajaram Swaminathan, Indian Institute of Technology, Guwahati, India, How Does SDS, CTAB and dtt Affect the Size, Rotational Dynamics and Growth Kinetics of Soluble Lysozyme Aggregates" David V. Svintradze, Tbilisi State University, Georgia...
DTT in Biological Technology

Increasing Economic and Business Risks Will Transform the Global Life Sciences Industry

...boration with The Economist Intelligence Unit, the dtt white paper, "The Future of Life Sciences Industri...ls to survive in the next decade," said Robert Go, dtt Life Sciences and Health Care Industry group leade...facing disruptive technologies," said John Rhodes, dtt Life Sciences Leader. "In the case of life science...

Deloitte Leadership Offers Crucial Convergence Considerations for Life Sciences Companies

...d to compete in the expanding marketplace." About dtt Life Sciences and Health Care Industry Group The dtt Life Sciences and Health Care (LSHC) Industry Grou...Fortune Global 500. For more information about the dtt LSHC Industry Group, email dttlshc@deloitte.com or...

Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

...ately in 1 mL of ammonium bicarbonate buffer (100 mM, pH 8.5) and 3 μL dtt (1 M, Sigma- Aldrich, St. Louis, MO, USA). The mixture was incubated for 30... an additional 30 minutes at room temperature in the dark. Thirteen μL dtt (1 M) was added to quench the iodoacetic acid. The reduced and alkylated pr...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

...e) PlusOne™ Bromophenol Blue 17-1329-01 PlusOne dtt 17-1318-01

PlusOne Glycerol ...

2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ

... 17-0854-01 PlusOne™ dtt 17-1318-01 ...rocedure. The proteins were dissolved by adding 1 ml of 9 M urea with 50 mM dtt to the tissue and allowing it to incubate for 60 min at 20 °C. A 1-ml a...

Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS

...lkylation, and trypsin digestion The pooled, dried fractions from the gel filtration step were dissolved in 0.5 ml of 9 M urea containing 50 mM dtt and incubated at ambient temperature for 60 min. An equal volume of a solution consisting of 8 M urea, 250 mM Tris-HCl, pH 8.8, and 125 mM iodoacetami...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...d 3 M ammonium sulfate precipitation. The resulting 3 M pellet was resuspended in 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, and 0.1 mM dtt (Buffer A) and then dialyzed overnight against two changes of buffer A plus 3 mM MgCl 2 (Buffer B). The dialyzed sample was cen...

Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A

...as set for rapid voltage ramping. Focused IPG strips were loaded onto 816% SDS-PAGE gels following equilibration for 10 min with dtt containing buffer followed by 10 min with iodoacetamide containing buffer. Following electrophoresis, gels were fixed for 30 min...

Modification of Bio-Rad DC Protein Assay for Use With Thiols

...tion for protein determination in the presence of reducing agents such as dtt (dithiothreitol) and BME (2-mercaptoethanol). This adaptation will effe...n in a buffer containing approximately the same concentration of dtt or BME as the sample. A standard curve should be prepared each t...

Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

...und in liquid nitrogen, thawed in 450 l of extraction buffer (0.1 M sodium phosphate, pH 7.8, 1% Triton X-100, 2 mM EDTA, 1 mM dtt and 0.1% BSA), vortexed and then centrifuged at 10,000 rpm in a microcentrifuge for 4 minutes. The supernatant is then transferre...
DTT in Biological Dictionary

Reducing agent

...tion of disulfide-containing peptides and proteins. ... is a potent, water-soluble, odorless reducing agent that is a popular alternative to dtt ... Crosslinking and Protein Modification > Reducing Agents > TCEP·HCl ... Reducing agent is not pyrophoric under ambient conditions ...

Immunoprecipitation

... procedure by which peptides or proteins that react ... Figure 1. Schematic representation of the principle of immunoprecipitation . ... Fresh dtt should be present throughout the immunoprecipitation . ... When the background in an immunoprecipitation is high, some people like to pre ... I...
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