Increasing Economic and Business Risks Will Transform the Global Life Sciences Industry
...boration with The Economist Intelligence Unit, the dtt
white paper, "The Future of Life Sciences Industri...ls to survive in the next decade," said Robert Go, dtt
Life Sciences and Health Care Industry group leade...facing disruptive technologies," said John Rhodes, dtt
Life Sciences Leader. "In the case of life science...
Deloitte Leadership Offers Crucial Convergence Considerations for Life Sciences Companies
...d to compete in the expanding marketplace." About dtt
Life Sciences and Health Care Industry Group The dtt
Life Sciences and Health Care (LSHC) Industry Grou...Fortune Global 500. For
more information about the dtt
LSHC Industry Group, email
Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis
...ately in 1 mL of ammonium bicarbonate buffer (100 mM, pH 8.5) and 3 μL dtt
(1 M, Sigma- Aldrich, St. Louis, MO, USA). The mixture was incubated for 30... an additional 30 minutes at room temperature in the dark. Thirteen μL dtt
(1 M) was added to quench the iodoacetic acid. The reduced and alkylated pr...
Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
PlusOne™ Bromophenol Blue
PlusOne Glycerol ...
2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
...rocedure. The proteins were dissolved by adding 1 ml of 9 M urea with 50 mM dtt
to the tissue and allowing it to incubate for 60 min at 20 °C. A 1-ml a...
Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS
...lkylation, and trypsin digestion
The pooled, dried fractions from the gel filtration step were dissolved in 0.5 ml of 9 M urea containing 50 mM dtt
and incubated at ambient temperature for 60 min. An equal volume of a solution consisting of 8 M urea, 250 mM Tris-HCl, pH 8.8, and 125 mM iodoacetami...
A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
...d 3 M ammonium
sulfate precipitation. The resulting 3 M pellet was resuspended in 50
mM Tris-HCl (pH 7.5), 1 mM EDTA, and 0.1 mM dtt
(Buffer A) and then dialyzed
overnight against two changes of buffer A plus 3 mM MgCl 2 (Buffer B).
The dialyzed sample was cen...
Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A
...as set for rapid voltage
ramping. Focused IPG strips were loaded onto 816% SDS-PAGE gels following
equilibration for 10 min with dtt
containing buffer followed by 10 min
with iodoacetamide containing buffer. Following electrophoresis, gels
were fixed for 30 min...
Modification of Bio-Rad DC Protein Assay for Use With Thiols
...tion for protein determination
in the presence of reducing agents such as dtt
and BME (2-mercaptoethanol). This adaptation will
effe...n in a buffer containing approximately the same concentration
or BME as the sample. A standard curve should be prepared each
Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
...und in liquid nitrogen, thawed in 450 l
of extraction buffer (0.1 M sodium phosphate, pH 7.8, 1% Triton X-100,
2 mM EDTA, 1 mM dtt
and 0.1% BSA), vortexed and then centrifuged at 10,000
rpm in a microcentrifuge for 4 minutes.
The supernatant is then transferre...