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DNase in Medical News

Genomatix integrates genome-wide open chromatin from next generation sequencing

...uary 2008 issue of Cell some remarkable work on High-Resolution Mapping and Characterization of Open Chromatin across the Genome where they identified dnase I hypersensitive sites from primary human CD4+ Cells. Those samples were sequenced using both the Illumina Solexa and Roche 454 NGS platforms. Geno...

Cystic Fibrosis foundation lays out new treatment guidelines

...nts with CF." The pulmonary guidelines committee assessed the clinical efficacy and safety of the use of aerosolized antibiotics, recombinant human dnase (dornase alfa), hypertonic saline inhalation, anti-inflammatory agents, macrolide antibiotics, the use of bronchodilators and N-acetylcysteine. They ...
DNase in Medical Products

10 L Pipette Tip (RNase, DNase and pyrogen free)

Description: 10 L, Racked, Pre-sterilized, 96 tips/rack Recommended for 5 & 10 L pipettes. Ideal for pipetting into long, narrow tubes or deep well plates....
Company:Hamilton Company

1 mL Filter Barrier Pipette Tip (RNase, DNase and pyrogen free)

Description: 1000 L, Racked, Pre-sterilized, 100 tips/rack Recommended for 500 and 1000 L pipettes....
Company:Hamilton Company

10 L Filter Barrier Pipette Tip (RNase, DNase and pyrogen free)

Description: 10 L, Racked, Pre-sterilized, 96 tips/rack Recommended for 2, 5 and 10 L pipettes....
Company:Hamilton Company

1 mL Pipette Tip (RNase, DNase and pyrogen free)

Description: 1000 L, Racked, 96 tips/rack, clear Recommended for 500 and 1000 L pipettes....
Company:Hamilton Company

300 L Pipette Tip (RNase, DNase and pyrogen free)

Description: 300 L, Racked, 96 tips/rack Recommended for 250 and 300 L pipettes. Can also be used with 20, 25, 50, 100 and 200 L pipette...
Company:Hamilton Company

10 L Extended Length Pipette Tips (RNase, DNase and pyrogen free)

Description: 10 L, Racked, 96 tips/rack Extended length pipette tips recommended for 5 and 10 L pipettes....
Company:Hamilton Company

10 L Pipette Tip (RNase, DNase and pyrogen free)

Description: 10 L, Stacked Rack, 96 tips/rack 5 racks/stack, clear Recommended for 2, 5, 10 L pipettes....
Company:Hamilton Company

300 L Pipette Tip (RNase, DNase and pyrogen free)

Description: 300 L, Bulk Recommended for 250 and 300 L pipettes. Can also be used with 20, 25, 50, 100 and 200 L pipette...
Company:Hamilton Company

200 L Pipette Tip (RNase, DNase and pyrogen free)

Description: 200 L, Racked, Pre-sterilized, 96 tips/rack, clear Recommended for 20, 25, 50, 100 and 200 L pipettes. Can also be used with 300 L pipettes when pipetting 200 L or less....
Company:Hamilton Company

ViralXpress™ DNA/RNA Extraction Reagent

Description:...ds; however, the kit does not separate DNA from RNA. Purification of either RNA or DNA can be achieved by incubating the purified material with either dnase or RNase, respectively, followed by re-extraction with ViralXpress™. The ViralXpress™ nucleic acid extraction kit can be used for the extrac...
Company:CHEMICON International

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DNase in Biological News

Search for the 'on' switches may reveal genetic role in development and disease

...nstitute. They combined two known processes to look at regulatory regions across the whole human genome, Crawford said. We used an enzyme called dnase that has been known for decades to preferentially identify unpackaged regions of DNA, he said. In this study, we identified all unpackaged regions wit...

Researchers expand efforts to explore functional landscape of the human genome

...and characterize regions of open chromatin through dnase I hypersensitivity assays, formaldehyde-assisted i...ion (four years); A Comprehensive Catalog of Human dnase I Hypersensitive Sites. This team will map and functionally classify dnase I hypersensitive sites across major human cell lin...

Protein clue to tailor-made antibiotics

... by destroying their DNA. Though most proteins have a folded structure, dnase colicins are only partially so. The scientists have found that the unfolded part of dnase colicin structure makes its way into an unsuspecting bacterium and blocks a...
DNase in Biological Technology

The World's Best DNase Improved TURBO DNA-free

Removes trace quantities of DNA that plague RT-PCR DNase enhancer increases the potency of TURBO DNase by 2-3 orders of magnitude Remove DNase from RNA solutions in 5 minutes without ...

An Animal-Free DNase I

Digestion of DNA to undetectable levels is important in many molecular biology applications. For instance, accurate quantitation of RNA targets by RT-PCR requires the removal of contaminating genomic DNA targets. In vitro transcribed RNA is also...

TURBO DNase: Hyperactive, Salt Tolerant DNase

Up to 50X more activity and 350% greater catalytic efficiency than conventional DNase I Removes trace quantities of DNA that can plague RT-PCR RNase-free and recombinant in ...

Quantitative Analysis of the Cell Cycle in Imaging Applications

...10 min. Afterwards, they were washed with the Perm/Wash Buffer, and re-fixed for 5 minutes. Cells were then washed with Perm/Wash Buffer, treated with dnase (Sigma-Aldrich, D4513, 15μg/well in PBS at 37C for 1 hour), washed in Perm/Wash Buffer and stained simultaneously with Alexa Fluor 488 (Invitroge...

Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents

...ew techniques that produce highpurity total RNA from biological sources such as cultured cells. These techniques eliminate the need for post-isolation dnase treatment or other manual handling, and produce a highly pure product, free of PCR inhibitors, that is less prone to degradation. These new ...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...PicoPure RNA Isolation Kit (Arcturus, Cat. #KIT0204) ◊ RNase-free dnase Set (Qiagen, Cat. #79254) ◊ RiboAmp HS RNA Amplification Kit (Arc... according to the instructions in the PicoPure kit, including the on-column dnase digestion detailed in the user guide Appendix A. Each RNA sample was eluted...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...Pure RNA Isolation Kit (Arcturus, Cat. # KIT0204) ◊ RNAse-Free dnase Set (Qiagen, Cat. #79254) ◊ Ribogreen RNA quantification kit (...nate potential genomic DNA contamination, RNA samples were treated with the dnase (Qiagen) directly onto the column as per manufacturers instructions. ...

DNA Yield with Oragene

...pecific Fluorescence/DNase method (ref. 5). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and dnase treatment. Results The DNA yield of Oragene/saliva samples from 208 donors is shown in Figure 2. The me...

DNA Stability with Oragene

...ific Fluorescence/DNase method (ref. 2, 3). The F/D method quantitates DNA using SYBR Green I dye (Molecular Probes, Inc.) and dnase treatment. Results DNA from Oragene/saliva samples stored at 24C and 37C had a molecular weight > 23,00...

Real-Time PCR: General Considerations, Rev A

...me that does not cut within the target cDNA RNA must be free from genomic DNA contamination treat with RNase-free dnase prior to reverse transcription. It is also helpful to design primers at splice junctions to avoid genomic DNA amplification ...
DNase in Biological Products

Sheep Anti-Staphylococcus DNase Polyclonal Antibody, Unconjugated from Abcam

Description: Sheep polyclonal to Staphylococcus DNase ( Abpromise for all tested applications). Antigen: Full length native protein (purified)....
Company:Abcam

Rabbit Anti-Human DNase II Polyclonal Antibody, Unconjugated from Calbiochem

Description: AVOID FREEZE/THAW CYCLES. Recognizes the ~32 kDa DNase IIα chain and the ~40 kDa proenzyme. DNase II causes both DNA condensation and DNA fragmentation. ...
Company:Calbiochem

RQ1 RNase-Free DNase from Promega

Description: RQ1 RNase-Free DNase is a preparation of deoxyribonuclease I that degrades single-stranded or double-stranded DNA to produce 3-hydroxyl oligonucleotides. This preparation is qualified for use in applications where maintaining the integrity of RNA is critical. RQ1 DNase is qualified for use with the...
Company:Promega

RNase-Free Dnase from BD Biosciences Pharmingen

Description: RNase-Free DNase 50 l...
Company:BD Biosciences Pharmingen

TURBO DNase (2 U/l) from Ambion

Description: TURBO DNase (patent pending) was developed using a protein engineering approach that introduced amino acid changes into the DNA binding pocket of wild-type DNase I. These changes markedly increase the affinity of the protein for DNA. The result is a versatile enzyme that has a 6-fold lower Km for D...
Company:Ambion

CELLection Epithelial Enrich from Dynal Biotech

Description:... The kit contains 5 ml CELLection Dynabeads (4.5 m) coated with the monoclonal antibody against the human epithelial antigen EpCAM and dnase Releasing Buffer. For up to 5 log enrichment of epithelial tumour cells directly from blood, bone marrow or MNC. Tumour cells clumps can be enriched ...
Company:Dynal Biotech

7.5 M LiCl Precipitation Solution from Ambion

Description:...eparations. Note, LiCl precipitation may not remove all DNA contamination from total RNA isolations. If the RNA is to be used for RT-PCR, it should be dnase I treated....
Company:Ambion

Disposable Tips for OneTouch Plus from The Gel Company

Description:... Disposable tips with floating filter (US Patent 6,702,990) for PDM1.5 OneTouch Plus spotpicker, 10 racks of 96, dnase and RNAse free...
Company:The Gel Company

UltraPure DNase/RNase-Free Distilled Water from Invitrogen

Description:... UltraPure DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-m membrane-filtered and tested for dnase and RNase activity....
Company:Invitrogen

UltraPure DNase/RNase-Free Distilled Water from Invitrogen

Description:... UltraPure DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-m membrane-filtered and tested for dnase and RNase activity....
Company:Invitrogen

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DNase in Biological Dictionary

DNase

A deoxyribonuclease ( DNase , for short) is any enzyme that catalyzes the ... The two main types of DNase found in metazoans are known as deoxyribonuclease I ... DNase Test - Gonorrhea - Lab information from CDC. ... The deoxyribonuclease ( DNase ) test detects the degradation o...

RT-PCR

... cleanup from PCR ... Molecular biology protocol: RT - PCR ... first strand cDNA for RT - PCR or other applications ... Protocol describes dnase treatment, the RT reaction and PCR . ... ... transcriptase ( RT ), however, can be combined with traditional PCR to allow ... The product of...

RNase

...er ... gene structure of Mus musculus RNase 6 and examine ... analysis reveals that RNase 6 was subject to ... Maintaining things RNase and dnase free - (reply: 2) Why should RNase treatment be followed by phenol chloroform - (reply: 4) ... RNase decomtamination - (reply: 6) ... Informati...

Nick translation

...eled by nick translation are used in many different hybrid- ization techniques. ... The nick translation method (1) is based on the ability of dnase I to ... Labeling principle The nick translation method (1) is based on the ability ... Probes prepared with the Biotin- Nick Translation Mi...

Gel shift assay

...e Mobility Shift Assay . 6 DNA EMSA Protocol. 7 DNA EMSA. 8 DNA EMSA Kits ... Gel shift assays are often performed in vitro concurrently with dnase ... ...

Deoxyribonuclease

...is an enzyme coded by the human gene DNASE1. dnase I is a nuclease that cleaves DNA preferentially ...HA) ... Deoxyribonuclease from beef pancreas, dnase I, first crystallized by Kunitz ... Deoxyribonucl... chops up the protein but ... Acronym Finder: dnase stands for Deoxyribonuclease ... Retrieved Augus...
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