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DNAs in Medical News

Scientists solve structure of gene regulator that plays key role in cancer

...ide densely knotted structures in the cell nucleus. There, the DNA is wound spool-like around proteins called histones, which bind to and restrict the dnas activity. For a cell to activate a gene, many proteins, called transcription factors, work in concert to separate DNA from the histones. One family of...

American Chemical Society's weekly PressPac -- Jan. 23, 2008

...e. And then like friends with similar interests, the bits of genetic material hangout or congregate together. The recognition of similar sequences in dnas chemical subunits occurs in a way once regarded as impossible, the researchers suggest in a study scheduled for the Jan. 31 issue of ACS Journal of P...

Human genetic variation -- Science's 'Breakthrough of the Year'

...cer, type 1 and 2 diabetes, heart disease, hypertension, multiple sclerosis and rheumatoid arthritis. In 2007, biologists also learned that within dnas billions of bases, thousands to millions of them can get lost, added or copied in ways that can change genetic activity within a few generations. The ...

If you think cancer genes are simple, you don't know JAK

...ut when its signals run amok, the system can play a role in the development of leukemia or lymphoma. For decades, scientists have been teasing out dnas secrets, learning how one snippet of DNA controls another, how some genes wield incredible power over others, and how the DNA code encrypts all kinds ...

Pre Implantation Genetic Haplotyping to Identify 6,000 Inherited Diseases

...ication to replicate the DNA. Then they blood samples from family members, including at least one person affected by the disease is collected and the dnas are compared. It is possible to identify the region of a chromosome that is responsible for the disease. The treatment costs £4,100. The Guy's team ha...

Evoprinter, a multigenomic comparative tool for rapid identification of functionally important DNA

...rived from the speed of a BLAT alignment) and the fact that only a single curated genomic sequence is required to initiate the analysis of orthologous dnas from multiple species. Based on the success of the Evoprinter identification of MCSs within known vertebrate and Drosophila cis-regulatory elements, t...
DNAs in Biological News

Researchers identify gene linked to aggressive progression of liver cancer

...ession of HCC, including invasion, metastasis, resistance to chemotherapy, the formation of new blood vessels, and senescence. cDNAs are complementary dnas that are generated from mRNAs to analyze gene expression profiles. "AEG-1 also activates multiple intracellular signaling pathways that are known t...

Leiden scientists sequence first female DNA

...er six months. History In 2001, the DNA sequence was published of a combination of persons. The DNA sequences of Jim Watson, discoverer of the dnas double helix structure, followed in 2007, and later the DNA of gene hunter Craig Venter. Recently the completion of the sequences of two Yoruba-Africa...

Genetic material under a magnifying glass

...on of Raman spectroscopy and atomic force microscopy. As reported in the journal Angewandte Chemie, Deckert and Elena Bailo have successfully analyzed dnas closest relative, RNA. Direct sequencing means that the letters of the genetic code are read directly, as if with a magnifying glass. A DNA or RNA ...

Case researcher in RNA biology makes waves by challenging current thinking

...des researchers have been puzzled by cells ability to differentiate between normal mRNA and those carrying certain types of mutations. mRNA transports dnas genetic coding information to the sites of protein synthesis: ribosomes. Cells are able to identify mRNA carrying a mutation and prevent it from reach...

Liquid crystal phases of tiny DNA molecules point up new scenario for first life on Earth

... in which the chains spontaneously oriented parallel to each other, he said. Researchers understand the liquid crystal organization to be a result of dnas elongated molecular shape, making parallel alignment easier, much like spaghetti thrown in a box and shaken would be prone to line up in parallel, Cla...

How did chemical constituents essential to life arise on primitive Earth?

... is passed on from generation to generation. First isolated in 1869 from the pus of discarded surgical bandages by Friedrich Miescher, a Swiss doctor, dnas double helix structure was solved by Watson and Crick in 1953. DNA is shaped somewhat like a twisted ladder with the rungs anchored by matching pairs ...

Exploring the dark matter of the genome

...ences that code for non-protein-coding RNAs and other functional elements, such as small RNAs that neutralize transposable elements, or transposons -- dnas similar to viruses that hop around the genome and are capable of disrupting gene function. "Drosophila is ideal for studying genomics for many r...

Regulating the nuclear architecture of the cell

...NA genes could not be silenced, rDNA proliferated. These and other repeated dnas became dispersed and disorganized throughout the cell nucleus. Instead of a...ference -- allows the heterochromatin to open up. The decondensed, repeated dnas are then free to interact; DNA recombination and repair processes excise th...

'Dating agency' boosts hunt for disease genes

...both data and materials about a disease. Then, they can 'date' a collaborator, design an experiment together and make an online wish list of patients' dnas that they need." The DNA Network provides web access by registered researchers to detailed summaries of data on patients whose anonymity is assure...

NYU chemists use computer simulation to enhance understanding of DNA transcription

...of chromatin strongly depends on its salt environment. To Schlick and her colleagues, these results revealed that in a low-salt environment, linker dnas in the array were repelled, preventing array folding and resulting in a bead-like structure. However, under high-salt conditions, screening of linker ...
DNAs in Biological Technology

UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs

Introduction The transfer efficiency of large (>100 kb) DNAs from agarose gels to membrane is poor unless the DNAs are nicked prior to transfer. Although this has traditionally been accomplished with HCl depurination, UV irradiation ...

DNA sensors found to be an effective artificial nose

...gnizes the molecule of interest. An example is the olfactory receptors in the main olfactory system of vertebrates. This study not only highlights dnas potential for use in a novel and powerful odor detection system, but it also highlights its potential to play other novel roles in vivo, for example ...

Center for Molecular Medicine Selected to Provide DNA Analysis for Children's Oncology Group Leukemia Study

...t can lead to highly targeted and specific therapies," he said. "In the COG study, the CMM will identify and catalog about a million key SNPs in these dnas from childhood leukemia patients so that the COG can move forward in its important work uncovering new approaches to identifying and combating this di...

New DNA-based technique for assembly of nano- and micro-sized particles

...lectron microscopy to visualize the clusters, as well as x-ray scattering at the National Synchrotron Light Source to study particles in solution, the dnas natural environment. ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... efficiency, giving rise to some loss of representation of dnas in this size range. Genomic DNA fragments less than ~400 bp will not ampl... upon a simple degenerate primer extension, it alone is poised to amplify dnas that are highly fragmented and/or modified. The DOP amplification mechani...

PFGE Analysis of Chromosomal DNA From a Single Hair Root

...n We have shown that DNA may be prepared from a single hair root and detected after pulsed-field electrophoresis. Smallersized dnas (e.g. < 20 kb) may be analyzed on conventional submarine gels using the same sample preparation method as the above analysis. Ba...

DGGE Analysis Using the DCode System, Rev B

...lowest domain is reached, the DNA will become partially melted, creating branched molecules. Partial melting reduces the dnas mobility in a polyacrylamide gel. Since the T m of a particular melting domain is sequence-specific, the presence of a mutation w...

PCR Optimization

...ep where PCR experiments fail. The temperature range for denaturation of most DNA samples is 94C to 96C. However, special target dnas require a different denaturation temperature to achieve an optimal result. The gradient would determine the lowest possible dena...

Normalizing cDNA libraries using the EppendorfThermomixer

...owing: Given the right circumstances, denatured, single-stranded dnas attempt to reassociate to double-stranded DNAs. Due to the likel...s via "frequent cutter" restriction enzymes. The single-stranded dnas were reamplified. This normalization procedure was repeated twic...

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

...ne an estimate of the yield of purified PCR product. The results are shown in Table 1. The mean clone total yield was 4955.9 ng. The dnas were resuspended at a concentration of ~200 g/ml in 25 l 50% DMSO-H2O. Many microarray spotters use 200 nl per print run and have a ...

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DNAs in Biological Definition

Microbiology

...ed them to be used in industry and as experimental tools in different branches of biology: Bacteria and simple eukaryotes contain small circular dnas , called plasmids . Plasmids can be genetically modified in vitro then inserted into bacteria that are grown in a culture to produce large amount...
DNAs in Biological Dictionary

S1 nuclease

...se can digest this single-strand loop and subsequently cleave the ... Moreover, S1 nuclease also cleaves heteroduplex molecules formed from the dnas ... S1 Nuclease Analysis. Experimental design considerations for promoter studies ... upon digestion with S1 nuclease following hybrid...

Gene family

...CT HOME ... Gene Expression - Roche. Generate more accurate and sensitive gene expression results ... Gene Mutation. Huge source of genomic dnas for SNP Methylation & Gene ... gene family ( ′jēn ′famlē ) ( genetics ) A group of related genes of similar sequence and function r...

Cosmid

...cribed here is being carried out at UGA. ... USA 83:4869-4873) N. crassa cosmid libraries (obtained from the Fungal Genetics ... The 110 cosmid dnas in question were distributed across three separate membranes. ... 14. Cosmid Finishing and Full Insert cDNA Sequencing Using Differential ... We ...

Convergent evolution

...n and other additional information on Convergent evolution from Biology-Online.org dictionary. ... Well can convergent evolution take place in dnas as well? ... Convergent evolution describes the acquisition of the same biological trait in ... The wing is a classic example of convergent ...

Branch migration

...tion Categories) from Hong Kong and Macao. We also consider all Temporary ... The branch migration substrates consist of two homologous duplex dnas each ... Branch migration to the opposite end of the duplexes results in complete strand ... These processes entail branch migration , whe...

Allele frequency

... data ... Download format for Arlequin: Allele frequency tables available in Arlequin ... To measure a SNP allele frequency in a mixture of dnas pooled from individual ... When the allele frequency is 50%, one expects that each of the two PCR ... We find that allele frequency restri...
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