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DIGE in Biological Technology

Ettan DIGE Imager

The Ettan™ DIGE Imager (Fig 1) is a scanning CCD camera designed for applications in the life sciences. In particular, it has been engineered to create high quality images of 2-D DIGE gels. By combining high resolution with precise motion control, the Ettan DIGE Imager p...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

Key words: ECL Plex • Ettan DIGE Imager • fluorescent Western blotting • multiplex detection • dynamic range The Ettan™ DIGE Imager is fully compatible with the ECL Plex™ system, reaching the same levels of sensitivity, ...

Imaging and Analysis of DIGE Product Information Sheet, Rev B

Difference gel electrophoresis (DIGE) is a relatively straightforward application of differential labeling of protein samples using fluorescent dyes. The technique, originally published by Unlu et al. (1997), uses cyanine (Cy) dyes to dif...

Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting

...aterial from a stripping procedure, providing more accurate data. Together with a high-end imaging system (e.g. Typhoon™ scanner or Ettan™ dige Imager), this results in high linearity and broad dynamic range for superior detection and quantitation of low-abundant proteins. ...

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