Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System
Clive R. Pullinger, Arghavan K. Shahidi, Andrea L. Verhagen and John
P. Kane *
Cardiovascular Research Institute, * Department of Medicine, Department
of Biochemistry and Biophysics, University of California, San Francisco,
Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE
David A. Crispin,* Ru Chen,* Michael B. Kimmey,** Teresa A. Brentnall**
* Departments of Pathology and
**Medicine, Division of Gastroenterology, University of Washington, Seattle,
Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes
...ytogenes that gave rise to two distinct
bands: one of which co-migrated with the single ba... Methods and Materials
THEORY OF dgge
is a technique that separates DNA fragments of the...
Detection of Mutations in the CYP21 Gene Using the DCode System
The results of the dgge
analysis of exons 2, 5, 6, and 7 are presented
in Figure 1. The presented dgge
analysis was carried out using a denaturing
undefined and rare point mutations. The dgge
analysis fulfills these criteria,
Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
... rDNA fragments.
The major advantage of dgge
is that it allows direct determination of
...produced by analyzing PCR products
in the manner described by Muyzer et al. 1 Six pe...u CCD camera (Hamamatsu, Japan). Image analysis of dgge
gels was performed using quantitative a...
Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
...arated on the basis of homoduplex mobility. We use dgge
and score both nuclear and mi...,
pers. comm.). Even without optimizing dgge
protocols, we detected four
to six diff...
analysis of PCR products amplified using universal...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
...n TTGE. One is based on results of a perpendicular dgge
gel. For example,
Borresen et al. cast ... of the S-shaped curve on the perpendicular dgge
gel. 10 Thus, a 50% denaturant
concent...the S-shaped curve on the perpendicular
requires a TTGE gel with 40% denaturant.
Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System
...conditions for separation of HLA-DRB alleles using dgge
established on a perpendicular den...a and 40% formamide).
The perpendicular dgge
was run in 1x TAE buffer at 60 C constant temperat...tant temperature. Individual bands on the parallel dgge
gels were visualised using SYBR-Green s...
TTGE on the DCode System
The focusing of bands obtained by dgge
seems to be retained, and in TTGE
Improvements of dgge
DGGE, which is based on the d...rolled to achieve
reproducible results. dgge
is complicated by the difficulties of choosing
Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System
the amplification process. 4, 5 dgge
was performed with the
DCode system. Th... determined by performing
according to the instruction manual (Bio-Rad). Ele... Separation of 16S rDNA amplified fragments by dgge
revealed the great
diversity in ground ...
Temperature Gradient Gel Electrophoresis (TGGE) and Denaturing Gradient Gel Electrophoresis ( DGGE
) are forms of electrophoresis where there is a temperature or chemical gradient across the gel. TGGE and DGGE
are useful for... Denaturing gradient gel electrophoresis ( DGGE
) is a common...
...re or chemical gradient across the gel . TGGE and dgge
are useful for... Denaturing Gradient Gel E...le since it helps to denature the sample, ... dgge
Denaturing gradient Gel Electrophoresis for de...ctrophoresis (DGGE) Celeste Peterson ... to run in dgge
gel , we tried to run in the agarose gel to see...
... 72°C for 7 min. Perform Steps 4 and ... denature . Typical PCR reaction. Chimera formation via partial extensions. Assumptions of clone and ... dgge
- Denaturing gradient gel electrophoresis ... NOTE: To denature Labeled DNA for a hybridization application, please see ... Do not heat- ...