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DGGE in Biological Technology

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

Clive R. Pullinger, Arghavan K. Shahidi, Andrea L. Verhagen and John P. Kane * Cardiovascular Research Institute, * Department of Medicine, Department of Biochemistry and Biophysics, University of California, San Francisco, California ...

Detection of Mutant K-ras in a Kindred With Hereditary Pancreatic Cancer by DGGE

David A. Crispin,* Ru Chen,* Michael B. Kimmey,** Teresa A. Brentnall** * Departments of Pathology and **Medicine, Division of Gastroenterology, University of Washington, Seattle, Washington. Introduction ...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...ytogenes that gave rise to two distinct dgge bands: one of which co-migrated with the single ba... Methods and Materials THEORY OF dgge dgge is a technique that separates DNA fragments of the...

Detection of Mutations in the CYP21 Gene Using the DCode System

... Results The results of the dgge analysis of exons 2, 5, 6, and 7 are presented in Figure 1. The presented dgge analysis was carried out using a denaturing ... undefined and rare point mutations. The dgge analysis fulfills these criteria, and t...

Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System

... rDNA fragments. The major advantage of dgge is that it allows direct determination of ...produced by analyzing PCR products with dgge in the manner described by Muyzer et al. 1 Six pe...u CCD camera (Hamamatsu, Japan). Image analysis of dgge gels was performed using quantitative a...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

...arated on the basis of homoduplex mobility. We use dgge to locate and score both nuclear and mi..., pers. comm.). Even without optimizing dgge protocols, we detected four to six diff... Discussion dgge analysis of PCR products amplified using universal...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

...n TTGE. One is based on results of a perpendicular dgge gel. For example, Borresen et al. cast ... of the S-shaped curve on the perpendicular dgge gel. 10 Thus, a 50% denaturant concent...the S-shaped curve on the perpendicular dgge requires a TTGE gel with 40% denaturant. ...

Separation of HLA-DRB Alleles by Denaturing Gradient Gel Electrophoresis using the DCode System

...conditions for separation of HLA-DRB alleles using dgge were established on a perpendicular den...a and 40% formamide). The perpendicular dgge was run in 1x TAE buffer at 60 C constant temperat...tant temperature. Individual bands on the parallel dgge gels were visualised using SYBR-Green s...

TTGE on the DCode System

...lems. The focusing of bands obtained by dgge seems to be retained, and in TTGE there...rform TTGE. Improvements of dgge by CDGE DGGE, which is based on the d...rolled to achieve reproducible results. dgge is complicated by the difficulties of choosing ...

Microbial Diversity in Ground and Surface Water Analyzed by Denaturing Gradient Gel Electrophoresis Using the DCode System

...during the amplification process. 4, 5 dgge was performed with the DCode system. Th... determined by performing perpendicular dgge according to the instruction manual (Bio-Rad). Ele... Separation of 16S rDNA amplified fragments by dgge revealed the great diversity in ground ...
DGGE in Biological Dictionary

DGGE

Temperature Gradient Gel Electrophoresis (TGGE) and Denaturing Gradient Gel Electrophoresis ( DGGE ) are forms of electrophoresis where there is a temperature or chemical gradient across the gel. TGGE and DGGE are useful for... Denaturing gradient gel electrophoresis ( DGGE ) is a common...

Denaturing Gel

...re or chemical gradient across the gel . TGGE and dgge are useful for... Denaturing Gradient Gel E...le since it helps to denature the sample, ... dgge Denaturing gradient Gel Electrophoresis for de...ctrophoresis (DGGE) Celeste Peterson ... to run in dgge gel , we tried to run in the agarose gel to see...

Denature

... 72°C for 7 min. Perform Steps 4 and ... denature . Typical PCR reaction. Chimera formation via partial extensions. Assumptions of clone and ... dgge - Denaturing gradient gel electrophoresis ... NOTE: To denature Labeled DNA for a hybridization application, please see ... Do not heat- ...
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