Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
... ◊ Tissue-Tek OCT Compound (Sakura, Cat. # 4538)
Treated Water (Ambion, Cat. # 9920)
Equipment and Labwar....
8. After 20 minutes, the slide is washed twice for 3 minutes in depc
9. The slide is dehydrated in 75%, 90%, and 100% et...
Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
...obic ruminal bacterium,
Prevotella bryantii, using an acid-phenol extraction procedure. 1 All solutions,
tubes, and pipets were depc
treated and autoclaved before use. Escherichia
coli K12 total RNA was isolated using a commercially available RNA purification
Which Water to Use?
High quality, depc
treated, and non-DEPC
treated water ... 1. DEPC-treated Water -
destroys enzymatic activity by modifying
...in RNases and
other proteins. depc
treatment is a very effective way to
Decontamination and inhibition of ribonucleases
..., glassware can be soaked in freshly prepared 0.1% depc
water or ethanol for 1 hour, drained, ...laved (necessary to destroy
any unreacted depc
which can otherwise react with other proteins and the
adenine residues of RNA). As depc
will attack polycarbonate (e.g. centrifuge
...ontrol ... Note: Hybridization temperature for Northern Blots with DIG Easy Hyb is 68 °C (for ... Northern Hybridization Solution. AB10055. depc
treated. Description ... Northern Hybridization Solution. 1 liter $155.66 © Copyright 2008 American Bioanalytical, Inc. ... Northern hybridi...