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DEPC in Biological Technology

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... ◊ Tissue-Tek OCT Compound (Sakura, Cat. # 4538) ◊ depc Treated Water (Ambion, Cat. # 9920) Equipment and Labwar.... 8. After 20 minutes, the slide is washed twice for 3 minutes in depc treated water. 9. The slide is dehydrated in 75%, 90%, and 100% et...

Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer

...obic ruminal bacterium, Prevotella bryantii, using an acid-phenol extraction procedure. 1 All solutions, tubes, and pipets were depc treated and autoclaved before use. Escherichia coli K12 total RNA was isolated using a commercially available RNA purification k...

Which Water to Use?

... High quality, depc treated, and non-DEPC treated water ... 1. DEPC-treated Water - depc destroys enzymatic activity by modifying ...in RNases and other proteins. depc treatment is a very effective way to ...

Decontamination and inhibition of ribonucleases

..., glassware can be soaked in freshly prepared 0.1% depc in water or ethanol for 1 hour, drained, ...laved (necessary to destroy any unreacted depc which can otherwise react with other proteins and the adenine residues of RNA). As depc will attack polycarbonate (e.g. centrifuge ...
DEPC in Biological Dictionary

Northern hybridization

...ontrol ... Note: Hybridization temperature for Northern Blots with DIG Easy Hyb is 68 °C (for ... Northern Hybridization Solution. AB10055. depc treated. Description ... Northern Hybridization Solution. 1 liter $155.66 © Copyright 2008 American Bioanalytical, Inc. ... Northern hybridi...
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