Amnis Announces the Release of Multiple Upgrades to the ImageStream(R) System
...stomers who purchase the 405nm laser can now
perform cell cycle, mitotic analysis and other experiments incorporating
the widely-used DNA binding dyes dapi
and Hoechst. The 658nm red laser
provides similar advantages for a broad range of red fluorescent dyes. The
laser options have been designed for field...
Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
...anti-GAPDH antibody. Red: Cy3 labeled siRNA; Blue: dapi
nuclei; Green: fluorescein labe... was analyzed using immunofluorescence.
(blue) stains cell nuclei; an antibody to GAPDH (g...ounted on microscope slides using VectaShield with dapi
examined using the appropriate fluo...
Streamline Your siRNA Transfections
... Transfection Agent. Cells were subsequently
fixed, stained with dapi
for visualization of nuclei, and analyzed by
fluorescence microscopy. Blue: dapi
stained nuclei; Red: Cy3 fluorescent
siRNA. (B) CDK2 siRNA or ...
siRNA Expression Vectors with Selectable Markers
weeks following selection, the cells were analyzed for GFP expression
by fluorescence microscopy. Green: GFP. Blue: dapi
stained nuclei. GFP
levels were remarkably reduced (94%) in cells transfected with the GFP
siRNA-encoding p Silencer 2.1-U6 hyg...
More siRNA Vectors for RNA Interference
antibody (Ambion). Green: anti-GAPDH antibody detected with fluorescein
labeled secondary antibody; Blue: dapi
The p Silencer 2.0-U6 and
3.0-H1 vectors each include four components: (1) a linearized and
Deliver siRNAs Into Primary Cells
and electroporated using hMSC-specific or PC-12-specific parameters. Cells
were fixed 24 hours after electroporation, stained with dapi
analyzed by fluorescence microscopy (Cy3 fluorescence; red).
Because knockdown of gene expressi...
pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
...lls two days after transfection
showing reduction of GAPDH levels in cells transfected with CMV-Gap. Green:
GAPDH protein. Blue: dapi
stained nuclei. (B) Relative levels of GAPDH
expression compared with HeLa cells not transfected (NT).
Figure 2. Exp...
Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
...rgeting survivin (BIRC5) or Silencer Negative Control #1 in quadruplicate.
Forty-eight hours later, two sets of samples were stained with dapi
analyzed by fluorescence microscopy. Total RNA was isolated from the other
two sample sets using RNAqueous MAG-96 and then c...
Confirming RNAi Effects Is Now Easier Than Ever
...vivin (BIRC5) or Silencer Negative Control #1 in
quadruplicate. Forty-eight hours later, two sets of samples were stained
and analyzed by fluorescence microscopy. Total RNA was isolated
from the other two sample sets using RNAqueous MAG-96 and then converted
Delivering siRNAs to Difficult Cell Types
(75 l) using hMSC-specific or PC-12-specific parameters. Cells were fixed
24 hours after electroporation, stained with dapi
(blue), and analyzed
by fluorescence microscopy (Cy3-fluorescence; red).
Figure 2. Silencing
NuCycl DAPI Kit from Exalpha Biologicals, Inc.
Description: NuCycl DAPI
Kit Application: Flow Cytometry Reactivity: Human...
Company:Exalpha Biologicals, Inc.
SlowFade Antifade Kit with DAPI from Molecular Probes (Invitrogen)
Description: SlowFade Antifade Kit with DAPI...
Company:Molecular Probes (Invitrogen)
SlowFade Light Antifade Kit from Molecular Probes (Invitrogen)
Description:...de Blue fluorophores that is often seen with the Slowfade reagent (S-2828). This kit is available with the blue-fluorescent nucleic acid counterstain dapi
included in the SlowFade antifade formulation (S-24636)....
Company:Molecular Probes (Invitrogen)
DAPI/Antifade Solution from CHEMICON
Description:... DAPI: 4', 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4g/mL dapi
suspended in Antifade....
4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Sigma-Aldrich
Description:...ich fluoresces approximately 20 times greater than dapi
is several times more sensitive than ethidium brom...DNA content during apoptosis using flow cytometry. dapi
staining has also been shown to be a sensitive and...
DAPI from KPL
(4,6-diamidine-2-phenylindole, dihydrochloride) is a fluorescent countersta...to red fluorochromes such as rhodamine, Cy3, Texas Red, or R-Phycoerythrin. dapi
can be used for fluorescent in situ hybridization, immunohistochemistry, an...
..... The Sleeping Beauty (SB) transposase reconstructed from salmonid fish has high ... SB transposase was found to be colocalized with intense dapi
(4′,6′-diamidino-2 ... The alignment of the transposase amino acid sequences from these IS1 family ... These results strongly suggest that IS...
...hen, at the leptotene /zygotene transition, a subset of recombinational ... ( 2) From early leptotene on rec8Δ chromosomes exhibit slightly fuzzy dapi
... At leptotene an axial core (element) assembles along replicated ... leptotene /zygotene of nearly all ... chromosome territories identiﬁed...