Studies on imaging and tracking transplanted cells

...ed green fluorescence protein (EGFP); B-galactosidase (LacZ); and live cell dapi nuclear staining. Each was used to label porcine bone marrow-derived MSCs i...lly with little ambiguity," commented Lee. The researchers concluded that dapi labeling could be reliably used for MSC tracking in both small and large an...

DAPI in Biological Technology

Amnis Announces the Release of Multiple Upgrades to the ImageStream(R) System

...stomers who purchase the 405nm laser can now perform cell cycle, mitotic analysis and other experiments incorporating the widely-used DNA binding dyes dapi and Hoechst. The 658nm red laser provides similar advantages for a broad range of red fluorescent dyes. The laser options have been designed for field...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...anti-GAPDH antibody. Red: Cy3 labeled siRNA; Blue: dapi stained nuclei; Green: fluorescein labe... was analyzed using immunofluorescence. dapi (blue) stains cell nuclei; an antibody to GAPDH (g...ounted on microscope slides using VectaShield with dapi and examined using the appropriate fluo...

Streamline Your siRNA Transfections

... Transfection Agent. Cells were subsequently fixed, stained with dapi for visualization of nuclei, and analyzed by fluorescence microscopy. Blue: dapi stained nuclei; Red: Cy3 fluorescent siRNA. (B) CDK2 siRNA or ...

siRNA Expression Vectors with Selectable Markers

...mycin. Three weeks following selection, the cells were analyzed for GFP expression by fluorescence microscopy. Green: GFP. Blue: dapi stained nuclei. GFP levels were remarkably reduced (94%) in cells transfected with the GFP siRNA-encoding p Silencer 2.1-U6 hyg...

More siRNA Vectors for RNA Interference

...p=262">GAPDH-specific antibody (Ambion). Green: anti-GAPDH antibody detected with fluorescein labeled secondary antibody; Blue: dapi stained nuclei. The p Silencer 2.0-U6 and 3.0-H1 vectors each include four components: (1) a linearized and ...

Deliver siRNAs Into Primary Cells

... and electroporated using hMSC-specific or PC-12-specific parameters. Cells were fixed 24 hours after electroporation, stained with dapi (blue), and analyzed by fluorescence microscopy (Cy3 fluorescence; red). Because knockdown of gene expressi...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...lls two days after transfection showing reduction of GAPDH levels in cells transfected with CMV-Gap. Green: GAPDH protein. Blue: dapi stained nuclei. (B) Relative levels of GAPDH expression compared with HeLa cells not transfected (NT). Figure 2. Exp...

Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success

...rgeting survivin (BIRC5) or Silencer Negative Control #1 in quadruplicate. Forty-eight hours later, two sets of samples were stained with dapi and analyzed by fluorescence microscopy. Total RNA was isolated from the other two sample sets using RNAqueous MAG-96 and then c...

Confirming RNAi Effects Is Now Easier Than Ever

...vivin (BIRC5) or Silencer Negative Control #1 in quadruplicate. Forty-eight hours later, two sets of samples were stained with dapi and analyzed by fluorescence microscopy. Total RNA was isolated from the other two sample sets using RNAqueous MAG-96 and then converted ...

Delivering siRNAs to Difficult Cell Types

...roporated (75 l) using hMSC-specific or PC-12-specific parameters. Cells were fixed 24 hours after electroporation, stained with dapi (blue), and analyzed by fluorescence microscopy (Cy3-fluorescence; red). Figure 2. Silencing Efficiency D...

DAPI in Biological Products

NuCycl DAPI Kit from Exalpha Biologicals, Inc.

Description: NuCycl DAPI Kit Application: Flow Cytometry Reactivity: Human...
Company:Exalpha Biologicals, Inc.

SlowFade Antifade Kit with DAPI from Molecular Probes (Invitrogen)

Description: SlowFade Antifade Kit with DAPI...
Company:Molecular Probes (Invitrogen)

SlowFade Light Antifade Kit from Molecular Probes (Invitrogen)

Description:...de Blue fluorophores that is often seen with the Slowfade reagent (S-2828). This kit is available with the blue-fluorescent nucleic acid counterstain dapi included in the SlowFade antifade formulation (S-24636)....
Company:Molecular Probes (Invitrogen)

DAPI/Antifade Solution from CHEMICON

Description:... DAPI: 4', 6-Diamino-2-phenylindole dihydrochloride. Antifade Solution: p-Phenylenediamine and glycerol in PBS solution. 0.4g/mL dapi suspended in Antifade....
Company:CHEMICON

4',6-Diamidino-2-phenylindole dihydrochloride (DAPI) from Sigma-Aldrich

Description:...ich fluoresces approximately 20 times greater than dapi alone. dapi is several times more sensitive than ethidium brom...DNA content during apoptosis using flow cytometry. dapi staining has also been shown to be a sensitive and...
Company:Sigma-Aldrich

DAPI from KPL

Description:... dapi (4,6-diamidine-2-phenylindole, dihydrochloride) is a fluorescent countersta...to red fluorochromes such as rhodamine, Cy3, Texas Red, or R-Phycoerythrin. dapi can be used for fluorescent in situ hybridization, immunohistochemistry, an...
Company:KPL

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DAPI in Biological Dictionary

Transposase

..... The Sleeping Beauty (SB) transposase reconstructed from salmonid fish has high ... SB transposase was found to be colocalized with intense dapi (4′,6′-diamidino-2 ... The alignment of the transposase amino acid sequences from these IS1 family ... These results strongly suggest that IS...

Leptotene

...hen, at the leptotene /zygotene transition, a subset of recombinational ... ( 2) From early leptotene on rec8Δ chromosomes exhibit slightly fuzzy dapi ... At leptotene an axial core (element) assembles along replicated ... leptotene /zygotene of nearly all ... chromosome territories identified...