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DABCYL in Biological Technology

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

...enzoic acid (DABCYL) can serveas a universal quencher for a number of fluorophores. Even if the fluorophoreemission spectrum does not overlap with the dabcyl absorption spectrum,quenching (with >95% efficiency) occurs by direct energy transfer fromfluorophore to quencher because of their close proximity. 2 ...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...quences 2425 nucleotides long, 6-nucleotide arm sequences, the fluorophore FAM covalently linked to the 5' end, and the quencher dabcyl linked to the 3' end. The melting temperatures of the hairpin stems and probe sequences were 6366C. To estimate the stability of...

HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA

...e fluorophore tetrachloro 6-carboxyfluorescein (TET). The IAC-specific molecular beacon is labeled on the 5 end with 6-carboxyfluorescein (FAM). dabcyl is attached to the 3 end both molecular beacons and is used to quench fluorescence. The HSV-TC and IAC molecular beacons are multiplexed in a si...

Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

...etrachloro 6-carboxyfluorescein (TET), and the mutant allele-specific molecular beacon is labeled with the fluorophore 6-carboxyfluorescein (FAM). dabcyl is used as the quencher on both molecular beacons. Fig.2 The assay is performed in a single PCR tube that contains a D...

Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits

...escein (TET), and the molecular beacon specific to the mutant allele is labeled with the fluorophore 6-carboxyfluorescein (FAM). dabcyl is used as the quencher on both molecular beacons. By using two different molecular beacons in each PCR reaction, three possible...
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