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CypHer5 in Biological Technology

The use of CypHer5 for receptor internalization studies in both a range of GPCRs and a non-GPCR

Introduction CypHer5 TM is a red-excited, pH-sensitive cyanine dye that is non-fluorescent at pH 7.4 and maximally fluorescent at pH 5.5. It is therefore ideally suited for monitoring internalization of cell surface receptors via acidic endosomal vesicles upon agonist...

Screening for β2-adrenergic receptor agonists using the pH-sensitive dye,CypHer5, and the IN Cell Analyzer 3000

...ody to the N-terminal epitope-tag is labelled with cypher5 and, on agonist stimulation, receptor internalizat...

Products used cypher5 labelled anti-VSV-G antibody, 250 mg ... > Method Live-cell cypher5 assay 1. Pre-co...

The use of CypHer5E and the IN Cell Analyzer 1000 for live-cell receptor internalization studies

...xpression levels than was previously possible with cypher5 dye.

Materials

...an increase in D:P ratio with CypHer5E compared to cypher5 due to the enhanced characteristics of the new dye... are comparable to values previously obtained with cypher5 dye and with other literature values (6).

Screening for potential beta 2-adrenergic receptor antagonists using CypHer5E and IN Cell Analyzer 1000

...ye, has shown utility in β2-adrenergic receptor agonist screening (1). cypher5 has been used to obtain dose-response and rank-order potency data for both ...Today 8 , 579585 (2003).

5. Application note: The use of cypher5 for receptor internalization studies in both a range of GPCRs and...

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