New Report Just Published: World Hepatitis C Virus And Therapeutics Market Report

...ses II-11 Immunomodulators in Pipeline by Company, and Development Stage II-11 Adjuvant Therapies to Compete with Mainline Therapies II-12 cyclophilin Analogs and Fibrosis/Inflammation Inhibitors in Pipeline by Company II-12 Vaccine Development Remains at Low Scale II-12 HCV Vaccines in P...

Study in Nature Medicine establishes major new treatment target in diseased arteries

...l Nature Medicine . By eliminating the gene for a signaling protein called cyclophilin A (CypA) from a strain of mice, researchers were able to provide complete p... protein to provide absolute protection, but it makes perfect sense because cyclophilin A promotes three of the most destructive forces in blood vessels oxidative...

Study in Circulation provides detail on how low blood flow promotes vascular disease

...of the journal Circulation . The work establishes cyclophilin A as an exciting target in the design of drugs aga...hree discoveries by providing strong evidence that cyclophilin A, a protein involved in the immune response, has ...e results because they provide solid evidence that cyclophilin A is at the center of it all." Study Details ...

Protein that provides innate defense against HIV could lead to new treatments

...am from Emory University School of Medicine, Vanderbilt University School of Medicine, and Mayo Medical School has identified CAML (calcium-modulating cyclophilin ligand) as the cellular protein that inhibits the release of HIV particles. CAML works by inhibiting a very late step in the virus lifecycle, lead...

Investigational drug tested for preventing muscle fiber death in muscular dystrophy

...ug, Debio-025, is a known inhibitor of the protein cyclophilin D, which regulates the swelling of mitochondria in...n alpha2. Similar to deleting the gene encoding cyclophilin D, we found that treatment with Debio-025 reduced ...cular dystrophy. This is why we believe inhibiting cyclophilin D could be a new treatment strategy, said Jeff Mol...

HIV Becomes More Infectious Due To Human Immune-system Protein

...ound that the human form of immune-system protein, cyclophilin A, changes the shape of HIV particles to make them...ells from Old World monkeys with HIV-1, found that cyclophilin A changed the shape of the virus particles, report...ith HIV-1. They found that the human form of cyclophilin A still changed the shape of the virus particles, ...

Cyclophilin in Medical Technology

Clinical Update - Debio 025 Cyclophilin Inhibitor for the Treatment of HCV

A Unique Viral Kinetics Profile LAUSANNE, Switzerland, April 19, 2007 /PRNewswire/ -- Debiopharm Group (Debiopharm), a global independent biopharmaceutical development specialist in oncology and serious medical conditions, presented additional results of a previous phase Ib, 15 day study with cycl...

Michael J. Fox Foundation Awards Over $2 Million for Validation of 10 Parkinson's Therapeutic Targets

...trick Aebischer, MD, Ecole Polytechnique Federale de Lausanne (Switzerland) and Deniz Kirik, MD, PhD, Lund University (Sweden) Validation of cyclophilin D, a protein involved in the mitochondrial permeability transi tion, as a target for Parkinson' s disease therapy Mathieu Lesort, PhD, Universi...

Clinical Update - Debio 025 in Hepatitis C

...conditions and particularly oncology, announced today the randomisation of its first patient in a phase IIb clinical study with Debio 025, a selective cyclophilin (Cyp) inhibitor with a potent anti-hepatitis C (HCV) effect. This multinational, double blind, placebo-controlled, parallel-group study will investiga...

Biotica Strikes Significant Collaboration Deal With GlaxoSmithKline

...ical validation. Biotica's programmes include rapamycin-based mTOR inhibitors (partnered with Wyeth), novel FK506 analogues for inhaled use in asthma, cyclophilin inhibitors and erythromycin macrolides (in collaboration with GlaxoSmithKline). All of Biotica's projects employ its proprietary technology, polyketid...

Clinical Update - Debio 025 in Hepatitis C

...5 days induced a strong anti-HCV effect (3.6 log10 reduction) in HIV-1/HCV co-infected patients. (Hepatology, Vol. 47, No 3, 2008, Flisiak et al. "The cyclophilin Inhibitor Debio-025 Shows Potent Anti-Hepatitis C Effect in Patients Coinfected with Hepatitis C and Human Immunodeficiency Virus)." About HCV HCV...

Cyclophilin in Biological News

Tips from the American Journal of Pathology

...mmation. Am J Pathol 2009, 174:177-183 cyclophilin B Is a Possible New Target for Treating Breast Can...ues at Northwestern University have uncovered that cyclophilin B may contribute to progression in breast cancer. ...The American Journal of Pathology . The protein cyclophilin B affects cell division, motility, and death, all ...

Mitochondria could be a target for therapeutic strategy for Alzheimer's disease patients

...ase patients. These new findings reveal that blocking this molecule, called cyclophilin D (CypD), and development of surrounding mitochondrial targets may be viabl...l death mediators and enhances production of reactive oxygen species (ROS). cyclophilin D (CypD), a type of enzyme called a prolyl isomerase that is located within...

JCI table of contents: Aug. 9, 2007

...e enhanced by agonists of beta-adrenergic receptors and abrogated by inhibitors of beta-adrenergic receptors. They were also abrogated in mice lacking cyclophilin D, which is the regulator of the mitochondrial permeability pore. This demonstration that Ca2+- and mitochondrial-dependent necrotic cardiomyocyte dea...

Cyclophilin in Biological Technology

Measuring siRNA-mediated knockdown of IL-8 mRNA

...ental results (data not shown), we determined that cyclophilin B (PPIB) demonstrated more stable expression than ...knockdown). To normalize the results, the IL-8 and cyclophilin B measurements made from the same replicate ( i.e....ysed cells) were converted to a ratio (IL-8 RLU to cyclophilin B RLU). This ratio was calculated for each of the ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...foam cells. Real-time quantitative RT-PCR for CD68 was performed on equivalent amounts of RNA (100 pg) and the results normalized to the control gene, cyclophilin A. As shown in Fig. 3C, the LCM-derived RNA was significantly enriched in the mRNA for the macrophage specific marker, CD68, compared to whole section...

Real-Time PCR: General Considerations, Rev A

...monstrates the importance of primer optimization using the human cyclophilin 40 gene. Two sets of primers, differing in location, were designed to amplify the same region of the human cyclophilin 40 gene (IMAGE Consortium clone 71154, ATCC). Figure 1 illustrat...

Nuclease Protection Assays: The Basics

...MAXIscript Kit with [- 32 P]UTP (800 Ci/mmol, 10 Ci/ml) and gel purified prior to hybridization. The specific activities of the cyclophilin and -actin probes were reduced twenty and 200-fold, respectively, by adding appropriate amounts of non-radioactive UTP to the tr...

Stabilizing RNA in Fresh and Frozen Tissues for RNA Isolation

...ame gel hybridized to radiolabeled probes for -actin, GAPDH, and cyclophilin (Note that the specific activity of the cyclophilin probe was lower than that of the other probes). For each tissue,...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

...ed plasmids and siRNAs targeting four different sequences in the cyclophilin and GAPDH genes. These nucleic acids were transfected into HeLa ...3' 45% 63% cyclophilin None 100% 100% ...

Enhanced siRNA Delivery and Long-term Gene Silencing

...n. For example, mRNA levels for ATM and cyclophilin were more strongly reduced when their r... negative control siRNA or an siRNA targeting cyclophilin were generated and used to in...n of the cells was harvested to analyze cyclophilin expression in cells that received the...

GENE SILENCING WITH PCR PRODUCTS

...y 500 ng of total RNA from each sample was assessed by Northern analysis. Radiolabeled RNA probes targeting 28S rRNA, GAPDH, and cyclophilin were used to detect target genes and normalize levels of input RNA. Films were exposed to the blots for 16 hours with a single i...

Control Your siRNA Research

...idated for use in human cell lines and each lot is functionally tested in an siRNA experiment. The GAPDH and cyclophilin siRNAs are also validated for use in mouse cell lines. These siRNA controls are ideal for developing and opt...

QuantiTect SYBR Green RT-PCR Kit

...ucibility and Sensitivity of the QuantiTect SYBR Green RT-PCR Kit One-step RT-PCR of a cyclophilin fragment from 100 ng, 1 ng, and 10 pg of total HeLa RNA. Twelve samples were amplified in parallel from each template amount. Coeffi...