Detection of mRNAs on Cryosections of the
Cardiovascular System Using DIG-Labeled RNA Probes
The following protocol was optimized from a protocol
using 35S-labeled RNA probes [1, 2]. In research studies,
it enables detection of the expression of rare mRNAs in
the cardiovascular system (e.g., of the proinflammator...
Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein
...croscopy prior to fixation and embedding in OCT (Figure 1). EGFP-labeled neural crest cells can be identified and captured by LCM from partially fixed cryosections
of embryonic tissue (Figure 2). Quantitative Real-Time PCR of the RNA extracted from these captured cells resulted in amplification of neural crest ce...
Your Data: RNAlater Stories
... Preserving RNA in cryosections
and Taking RNAlater into Space
treating tissue cryosections
with RNAlater so that
the sec... Preserving Intact RNA in
Rolf Jaggi*, ...
Roche Diagnostics Corporation
A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
Detection of mRNAs on cryosections
of the Cardiovascular System Using DIG-Labeled RNA Probes
Select the Optimal Enzyme for RT-PCR
Laser microdissection and nucleic acid purification - a Leica - QIAGEN
detected one cycle earlier than when using the protocol for cryosections
(Figure 3B). Low-abundance transcripts, such as TNF-alpha, can be ...pe of lasermicrodissected
tissue, TNF- mRNA was easily detected in cryosections
but was not
detected after 40 cycles with RNA isolated from formal...