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Cryosections in Biological Technology

Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes

The following protocol was optimized from a protocol using 35S-labeled RNA probes [1, 2]. In research studies, it enables detection of the expression of rare mRNAs in the cardiovascular system (e.g., of the proinflammator...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...croscopy prior to fixation and embedding in OCT (Figure 1). EGFP-labeled neural crest cells can be identified and captured by LCM from partially fixed cryosections of embryonic tissue (Figure 2). Quantitative Real-Time PCR of the RNA extracted from these captured cells resulted in amplification of neural crest ce...

Your Data: RNAlater Stories

... Preserving RNA in cryosections and Taking RNAlater into Space ...technique for treating tissue cryosections with RNAlater so that the sec... Preserving Intact RNA in cryosections with RNAlater Rolf Jaggi*, ...

Roche Diagnostics Corporation

...s A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage Detection of mRNAs on cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes Select the Optimal Enzyme for RT-PCR PCR-G...

Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation

...s were detected one cycle earlier than when using the protocol for cryosections (Figure 3B). Low-abundance transcripts, such as TNF-alpha, can be ...pe of lasermicrodissected tissue, TNF- mRNA was easily detected in cryosections but was not detected after 40 cycles with RNA isolated from formal...
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