DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
...cator of DNA damage and repair is of great interest to groups investigating these pathways (Kim et al . 2005). The assay is generally performed using coverslips and chamber slides and imaged on fluorescence microscopes (Sedelnikova et al . 2004). The demands for high spatial resolution and sophisticated image...Inducing RNAi with siRNA Cocktails Generated by RNase III
... Transfections HeLa cells at 30,000 cells per well, or 293 cells at 50,000 cells per well, were grown on glass coverslips in a 24 well tissue culture plate and transfected with siRNA at the indicated concentrations using siPORT Li...Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
...5 x 104 cells/cm2 on laminin-coated glass coverslips (16 mm). Cultures were maintained in neurobasal B2...with 1% fetal calf serum. After neuron attachment, coverslips were transferred (cell-side down) to dish... Effectene Reagent complex formation, the coverslips containing the neurons were removed from the glial...Sample Preparation for Microinjection
... a glass coverslip (10 x 10 mm). Place coverslips into a humid chamber and incubate at 37C until cells attach to the glass (usually takes 68 hr). Transfer coverslips into 35 mm petri dishes containing 2 ml of culture mediu...Printable CELLocate Log Sheets for Easily Locating Adhererent Cells
... CELLocate is the ideal solution for relocating adherent cells that have been used for microinjection. These sterile coverslips come with a microgrid pattern. Using the CELLocate protocol blocks with the original microgrid patterns it is easy to mo...Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
...uring specifications and the appropriate range for reagent volume and concentration of the plasmid DNA. Following transfections, cells were fixed, coverslips were prepared, and fluorescent cells were counted. 4 Figure 4 A quantitative analysis of transfection effici...Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
...incubated overnight. Cells were then fixed with 4% paraformaldehyde at room temperature for 25 minutes and rinsed with deionized, distilled water. coverslips with attached cells were mounted in glycerol containing 2.5% 1,4-diazabicyclo(2.2.2)octane (DABCO) then viewed and photographed using a Nikon Mi...BD BioCoat Osteologic 12 mm round Coverslips from BD Biosciences - Discovery Labware
Description: BD BioCoat Osteologic Coverslips that have been sterilized by ETO gas. Coating : Osteologic&trade Thickness : 0.16 mm Diameter : 12.7 mm Gridded : No ...Large Volume UltraMount from Lab Vision
Description:...aMount is formulated for coverslipping sections stained with alcohol soluble chromogens, such as Fast-Red or AEC. No heating is required prior to use. coverslips may be removed by soaking in water....ApopTag® Plastic Coverslip from CHEMICON
Description:... APOPTAG® Plastic coverslips are designed to be custom fitted to the specific APOPTAG® kit application. Each coverslip has 2.5 x 1 dimensions....Crystal / Mount mounting medium - general purpose from GeneTex
Description:...tages of Crystal/Mount are as follows: Produces permanently mounted specimens. Universal mounting medium. Produces superior specimens. Cost effective. coverslips are not required. Tissue sections can be post mounted and coverslipped using any organic mounting media....Microarray Gene Frame (330uL) from ABgene
Description:...e hybridisations. These frames feature the same dual strength adhesive system as our standard Gene Frame® range for easy removal after use. Rigid coverslips ensure hybridisation uniformity and there are three sizes available. Applications Microarray Slide Hybridisations...