Chromatin

Genomatix integrates genome-wide open chromatin from next generation sequencing

Genomatix Software GmbH, a Systems Biology company focussed on high quality annotation and the understanding of gene regulation, has begun showcasing its abilities in the analysis of data generated by Next Generation Sequencing (NGS) technology. Allan P. Boyle et al published in the January 200...

Stem Cells' "Suspended" State Preserved by Key Step, Scientists Report

...tects DNA in the cell nucleus. This step, known as chromatin remodeling, allows the cell's protein-making machi...d bone. A number of genes are known to trigger chromatin remodeling, allowing small sections of DNA to beco...uripotent stem cell state. The team plans to study chromatin remodeling in still more detail to clarify what ot...

BRIT1 allows DNA repair teams access to damaged sites

...d with proteins called histones to form chromatin. chromatin is a very condensed structure that forms a natural...-doctoral fellow in Systems Biology. ATP-dependent chromatin remodeling is a fundamental mechanism used by cells to relax chromatin in DNA repair, but the detailed molecular mechanis...

Novel DNA vaccine leads to kidney damage prevention in systemic lupus erythematosus models

...N-gamma (p=0.026, statistically significant). Interestingly, the prevalence and levels of other autoantibodies such as anti-Argonaute2 (Ago2)/Su and chromatin did not differ between the groups, indicating that the vaccination was Sm antigen-specific and successfully targeting particular antibodies. Significa...

Wistar Institute team finds key target of aging regulator

...ur aging model enables us to do many discovery screens that are impossible with other, more complex organisms. Yet it is remarkable that many of these chromatin mechanisms associated with yeast could turn out to be relevant even for aging human cells." ...

Farnesoid X receptor regulates cystathionase

... Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), chromatin Immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the regulation of CSE transcription. The molecular mechanism of the CSE a...

Chromatin in Medical Technology

Artelis and Selexis Report Record Antibody Production Levels in CHO Cells of Over 31 Grams Per Liter

...is.be . About the Selexis Genetic Elements(TM): Selexis Genetic Elements (TM) are applied to control the dynamic organization of transgenes in the chromatin of eukaryotic production cells. The Selexis Genetic Elements(TM) enable the creation of loops of open chromatin upon the stable integration of the SGE...

Chroma Announces Positive Phase I Efficacy Data for Novel Cancer Therapy CHR-2797

...he fields of oncology and inflammatory disorders. Chroma is building a broad pipeline of first- or best-in-class treatments utilising its expertise in chromatin biology and its novel intracellular accumulation technologies, which include the ability to selectively target drugs to macrophages. Chroma is backed ...

DNA Therapeutics Reports a Novel Mechanism of Action and a Biomarker of its Lead Molecule Dbait That Open an Innovative Pathway-Targeting in Molecular Cancer Therapy

...iphering the underlying mechanism of action of Dbait at molecular and cellular levels, and in particular its impact on DSB repair foci organization at chromatin level. It is known that the kinases ATM/ATR and DNA-PK are involved in the general cellular response to DNA damage by signaling proteins that contro...

Chromatin in Medical Products

Jenner Stain

Description:...day. It provides results similar to that of Giemsa stain, although eosinophillic granules tend to appear brighter red; basophilic granules and nuclear chromatin stain a dense blue....
Company:Richard-Allan Scientific

Giemsa Stain

Description:... used on peripheral blood smears and bone marrow. Erythrocytes stain pink to buff with platelets exhibiting a very light, pale pink. Leukocyte nuclear chromatin stains magenta-purple, monocyte cytoplasm stains the classical "ground glass" blue and lymphocyte cytoplasm stains clear, sky blue....
Company:Richard-Allan Scientific

Chromatin in Medical Dictionary

Chromosomes

...ividual strands of chromosomes . ... Biology4Kids.com! The web site that teaches the basics of biology and life science to everyone! ... When the chromatin comes together, you can see the chromosomes . ... Run a search on Chromosomes at Wikipedia. Search Wikimedia Commons for images, sounds and oth...

Chromatin in Biological News

SUMO protein guides chromatin remodeler to suppress genes

BOSTON (April 27, 2009) In an in vitro study, led by Grace Gill, PhD, Tufts University School of Medicine, researchers discovered how a protein called SUMO (Small Ubiquitin-related Modifier) guides an enzyme complex that alters the structure of chromatin to regulate expression of genes. Chromatin ...

Protein function and chromatin structure methods featured in Cold Spring Harbor Protocols

COLD SPRING HARBOR, N.Y. (Tues., March 3, 2009) Two new methods for analyzing the roles played by proteins in cells are featured in the March issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc3_09.dtl ). Thomas J. Wandless and colleagues from Stanford University ( http://w...

New technique yields more detailed picture of chromatin structure

University of Illinois researchers have developed a technique for imaging cells under an electron microscope that yields a sharper image of the structure of chromatin, the tightly wound bundle of genetic material and proteins that makes up the chromosomes. Their findings appear in Nature Method...

UC San Diego engineer provides insights to decades-old DNA squabble

...ciences (PNAS) called "Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions." ...New York University. The internal structure of chromatin is not knowna mystery that has baffled scientists for more than three decades. chromatin is a complex combination of DNA and proteins that ...

LincRNAs serve as genetic air-traffic controllers

... planes in the air, lincRNAs may be organizing key chromatin complexes in the cell." Inspired by a lincRNA called HOTAIR -- which is known to bind key chromatin modifier proteins and to assist in getting these p...ng which areas to compact and which to leave open, chromatin successfully determines which genes are accessible...

Pushmi-pullyu of B-cell development discovered

...hose epigenetic factors. They suspected that two chromatin remodeling complexes, conglomerations of several d... cell-culture experiments they showed that the two chromatin remodeling complexes, SWI/SNF and Mi2/NuRD, had op...70-fold when a cell contained both EBF and the two chromatin remodeling complexes. When the researchers inactiv...

Chromatin in Biological Technology

Syngenta Licenses Chromatin Gene Stacking Technology for Sugar Cane

RESEARCH TRIANGLE PARK, N.C., June 26 /PRNewswire/ -- Syngenta Biotechnology, Inc. announced today that it has entered into an exclusive worldwide research and commercial license agreement for Chromatin's proprietary gene stacking technology in sugar cane. Chromatin has developed a novel ap...

Syngenta Licenses Chromatin Gene Stacking Technology

RALEIGH, N.C., Oct. 19 /PRNewswire-FirstCall/ -- Syngenta Biotechnology Inc. announced today that it has entered into a research and commercial license agreement for Chromatin's proprietary gene stacking technology. Chromatin has developed a novel approach to gene stacking, using the plant's ...

Sirnaomics Receives SBIR Grant from NIH/NCI for Its Multi-Targeted Lung Cancer siRNA Therapeutics

...l phenomenon and as a versatile, powerful tool for biomedical research. RNAi plays a multifaceted role in molecular biology by silencing genes through chromatin remodeling, interfering with protein synthesis, and in its best-studied mode of action quashing gene expression by cleaving messenger RNA. Experimenta...

Stem cells' 'suspended' state preserved by key step, scientists report

...tects DNA in the cell nucleus. This step, known as chromatin remodeling, allows the cell's protein-making machi...nd bone. A number of genes are known to trigger chromatin remodeling, allowing small sections of DNA to beco...uripotent stem cell state. The team plans to study chromatin remodeling in still more detail to clarify what ot...

Selexis Launches Biosimilar Cell Line Development Program

...'s core intellectual property portfolio is based on the discovery and application of novel DNA based elements that control the dynamic organization of chromatin within all mammalian cells. Application of the Selexis Genetic Elements(TM) using the SURE Cell Line Development(SM) process has enabled the rapid dev...

Frost & Sullivan Awards Genpathway 2008 Healthcare Innovation Award

...t services and develops novel diagnostic biomarkers that predict drug responses, enabling personalized treatments. The company applies its proprietary chromatin and methylated DNA immunoprecipitation (ChIP) technology to understand gene regulation and genetic pathways by studying transcription factor binding, ...

Chromatin in Biological Products

Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)

Description:...vely and negatively supercoiled DNA in presence of EDTA Applications Studying the effects of supercoiling on transcription in vitro Studying chromatin reconstitution in vitro Determining the degree of supercoiling of naturally occurring DNA Detecting mutant plasmids that differ in length by onl...
Company:Molecular Cloning Laboratories (MCLAB)

ChIP-PCR for NFkb from Aviva Systems Biology

Description:...or ChIP assays. Efficient fixation of a protein to chromatin in vivo is a crucial step for the Chromatin-immuno...tigen epitopes to formaldehyde. For rapid checking chromatin IP efficiency. DNA from an aliquot of IP-chromatin...oter regions can be performed to check whether the chromatin immunoprecipitation is successful....
Company:Aviva Systems Biology

Mouse Anti-HDAC8 Monoclonal Antibody, Unconjugated, Clone HDAC8-48 from GeneTex

Description:... Mouse monoclonal [HDAC8-48] to HDAC8 Regulation of gene expression is mediated by several mechanisms such as DNA methylation, ATP-dependent chromatin remodeling, and post translational modifications of histones, which include the dynamic acetylation and deacetylation of epsilon-amino groups of lysin...
Company:GeneTex

EZ-Chip from Upstate

Description:... From the company that first brought you the chromatin immunoprecipitation (ChIP) kit, a new and improved kit, EZ-ChIP, is now available to make your experiments easier, quicker, and most importantly, more...
Company:Upstate

Calf Topoisomerase I from USB Corp.

Description:...5 units/l Applications: *Analysis of DNA supercoiling and conformation (2) . *Produces knots in single-stranded, circular DNA (2) . *Analysis of chromatin reconstitution in vitro (4) . *DNA repair studies (4) . *Studies on drug resistance, cell proliferation and leukemia (5,6) ....
Company:USB Corp.

S. cerevisiae yeast Whole Genome from Agilent Technologies

Description:...The Yeast Whole Genome ChIP-on-chip Microarray is specifically designed for location analysis of yeast (S. cerevisiae) DNA binding proteins by pairing chromatin immunoprecipitation (ChIP) with Agilent DNA microarrays. This set delivers robust hybridization and reliable binding data with greater true binding ev...
Company:Agilent Technologies

Chromatin in Biological Definition

Chromatin

Chromatin is the substance of a chromosome and consists of a complex of DNA and protein in eukaryotic cells . It can be made visible by staining (thus the name which literaly means coloured material). The nucleic acids are generally in the form of double-stranded DNA. The major pro...

Apoptosis

... ) that have been activated inside the cell. chromatin ( DNA and its packaging proteins ) in the cell...e by Madeleine Kihlmark et al. , in [2] ). chromatin undergoes further condensation into compact patche...ed, also, that while the previous stage of initial chromatin condensation has been observed in nonapoptotic for...

Cell nucleus

...ope . (2) Ribosomes . (3) Nuclear pore complexes. (4) Nucleolus . (5) chromatin . (6) Nucleus. (7) Endoplasmic reticulum. (8) Nucleoplasm . The whole str...present in the nucleus, the DNA is present as a DNA-protein complex called chromatin . The DNA is present as a number of discrete units known as chromosomes . ...

Chromosome

.... 1), and where this composite material is called chromatin . During mitosis (cell division), the chromosome...n eukaryotes 2 Chromosomes in bacteria 3 chromatin 4 Chromosomes in different species 5 Kar...e replication and one origin of replication. chromatin Two types of ...

Disulfide bond

...lysosomal proteins, and the exoplasmic domains of membrane proteins. Disulphide bonds are also formed within and between protamines in the sperm chromatin of many mammalian species. In hair Hair is a biological polymer, with over 90% of its dry weight made of proteins called keratins . Under...

Epigenetics

...ars, there has been rapid progress in understanding epigenetic mechanisms, which include differences in DNA methylation , as well as difference in chromatin structure. Another possibility involves the genomes of cytoplasmic elements ( chloroplasts and mitochondria ). Other mechanisms have also been prop...

Chromatin in Biological Dictionary

Chromatin

Chromatin is the complex combination of DNA, RNA, and protein that makes up chromosomes. It is found inside the nuclei of eukaryotic cells, and within the nucleoid in prokaryotic cells. It is divided between heterochromatin... chromatin n. A complex of nucleic acids and proteins, primari...

Triplet repeat expansion

... that the GAA triplet - repeat expansion may result in an unusual ... Triplet repeat expansion in myotonic dystrophy alters the adjacent chromatin structure. ... is caused by an expansion of a CTG triplet repeat sequence in the 3' ... GAA Triplet – Repeat Expansion as an Impedimen...

Satellite DNA

... a component ... A purification scheme for satellite DNA containing chromatin from mouse liver ... Satellite DNA content of chromatin fractions isolated from Eco R1-digested mouse ... Satellite DNA s...

Ribosomal DNA genes

...l silencing in yeast ribosomal DNA . Genes Dev. ... Transcription of Multiple Yeast Ribosomal DNA Genes Requires Targeting of UAF to ... chromatin Assembly onto Active Ribosomal Genes in Yeast after UV ... ... counterpart of metaphase NORs, therefore being constituted by ribosomal DNA...

Regulatory region

...main of the polymerase is ... IPR004102 Poly(ADP-ribose) polymerase, regulatory region 662 - 798 ... ... of the regulatory role played by chromatin organisation in the 5' region . ... The observation that the 5' regulatory regions of Hkg are less enriched in S ... ... of this type is the ...

Prophase

... Prophase is a stage of mitosis in which the chromatin condenses into a highly ordered structure called a chromosome in which the chromatin becomes visible. This process, called chromatin condensation, is mediated by the condensin comple...