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Chloroform in Medical News

Anti-microbial catheter to cut infection risk for dialysis patients

...chloroform, and the anti-microbial agents which causes the silicone to swell to twice its normal size, making these gaps between molecules bigger. The chloroform is then removed and the device shrinks back to its normal size encasing the anti-microbial agents inside but allowing them to move through the silicon...

Natural Solutions May Issue Illustrates 'Cocktail Effect' of Everyday Toxins; Offers Tips to Reduce Toxic Load

...ural or organic personal care products for adults and babies. -- Triclosan -- an ingredient in antibacterial soaps and detergents that emits chloroform gas when combined with chlorinated water and can cause liver and kidney damage. Avoid antibacterial products. -- Benzene -- a petrochemical foun...

Link between chlorine byproducts and pregnancy risks

...ct drinking water.// When it is added to water that contains organic matter such as runoff from farms or lawns, however, it can form compounds such as chloroform that can cause illness. The study released by the Environmental Working Group and Public Interest Research Groups identified areas that may have...
Chloroform in Biological News

Fair trade

...processes used in the production of other chlorinated hydrocarbons, such as chloroform (trichloromethane, CHCl3), also produce CCl4 as a byproduct. What is the be... atoms behind on the catalyst surface. Both of these reaction steps produce chloroform exclusively; no byproducts come into play. This new catalytic reaction is...

Chloroform provides clue to 150 year old medical puzzle

...sis [the furring up of arteries] and it just so happened that the lipids I was using were supplied already dissolved in chloroform. I noticed that the chloroform inhibited, or blocked, the calcium ion channel TRPC5 it was quite a striking effect. Ion channels are pathways that allow electrically charged ato...
Chloroform in Biological Technology

EFFECTS OF INSTRUMENT RESOLUTION ON ASSESSMENT OF DNA PURITY

...enol. Contamination by protein is usually indicated by a decrease in the A 260 /A 280 ratio. Contamination by an extraction solvent such as phenol or chloroform is indicated by increased absorbance at 320nm. If the criterion for acceptable resolution of instrument bandwidth ten-fold less than peak wavelength d...

Quantitation of a Dinitro Flavonoid (DNF) in Rat Plasma Using the Varian 1200L LC/MS/MS System

... containing DNF is spiked with 10 μL of flavone (0.2 ng) as the internal standard (ISTD). The rat plasma is made basic and extracted with hexane: chloroform (2:1, v/v). After separation of the phases by centrifugation, the organic layer is transferred by pouring into a silanized conical tube and ...

Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

... fortified with CAP in the concentration range of 0.05 1.5 μg/L. The cleanup consisted of an acetonitrile extraction, followed by defatting with chloroform and then solid phase extraction on a styrene-divinyl benzene polymer cartridge (Table 1). The final extract was analyzed on an API 3000 LC/MS/MS syste...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...e sections were coverslipped and submerged overnight in 60 o C hot Mineral oil to avoid evaporation. The next day the sections were dipped 10 times in chloroform and de-coverslipped in 50C warm 2x SSC buffer. After a 1 h wash in 2xSSC, non-specifically bound probe was digested in RNase buffer with 20g/mL RNase ...

Transgenic Zebrafish by Electroporation

..., pH 8.0) and 100 g are digested overnight with BamHI at 37 C. The digest is extracted once with phenol/chloroform and once with chloroform alone. The DNA is precipitated from the aqueous phase using absolute ethanol. This DNA is then resuspended in calcium-free phosp...

RNA, DNA, and Protein from a Single Sample

..., and spleen and from fresh cultured human cells using the standard TRI reagent protocol with either bromochloropropane (BCP) or chloroform (CHCl3). (A) Genomic DNA removal was assessed by one-step, (+) or () RT, quantitative PCR targeting mouse or human TATA binding...

Improve RNA Isolation for Array Analysis and More

... href="http://www.ambion.com/catalog/CatNum.php?9736">RNAwiz , which contains phenol and RNase inhibitors, then adding chloroform and mixing. The sample is then centrifuged to separate organic and aqueous phases. The aqueous phase, which contains the RNA, is...

Characterization of Protein Oligosaccharides Using MSn

...des were then dissolved in a slurry of sodium hydroxide and dimethyl sulfoxide, permethylated with methyl iodide, and extracted into chloroform per Ciucanu and Kerek. 3 LCQ Conditions Ionization method: ESI Sample introduction: direct infusion...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

...Heavy and incubate 5 minutes at 1530C. Add 0.2 ml chloroform (or chloroform-isoamyl alcohol) per 1 ml TRIzol Reagent initia...se Lock Gel layer. If this is not the case, add another 0.2 ml chloroform (or chloroform-isoamyl alcohol) per 1 ml TRIzol Reagent used i...

Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy

...Heavy and incubate 5 minutes at 1530C. Add 0.2 ml chloroform (or chloroform-isoamyl alcohol) per 1 ml TRIzol Reagent initia...se Lock Gel layer. If this is not the case, add another 0.2 ml chloroform (or chloroform-isoamyl alcohol) per 1 ml TRIzol Reagent used i...
Chloroform in Biological Products

Gel extraction kit from BioPioneer

Description:...A can be used in any downstream application such as sequencing, labeling, restriction enzymatic digestion, ligation or transformation. * No phenol / chloroform extraction or ethanol precipitation is required....
Company:BioPioneer

UltraClean Plant DNA (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:...r DNA from plant cells and tissues. The bead beating technology used in this kit replaces cumbersome DNA isolation procedures such as CTAB, phenol, or chloroform extraction. Using the UltraClean procedure, alcohol precipitation is not necessary - purified DNA is ready for immediate use. Plant tissue samples a...
Company:Mo Bio Laboratories, Inc.
Chloroform in Biological Dictionary

RNase

...reveals that RNase 6 was subject to ... Maintaining things RNase and DNase free - (reply: 2) Why should RNase treatment be followed by phenol chloroform - (reply: 4) ... RNase decomtamination - (reply: 6) ... Information about R.E.D.D. - Rnase -L Enzyme Dysfunction Disease, the condition underlyi...
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