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Chloramphenicol in Medical News

Green Tea Boosts Antibiotics for Superbugs

...n a prepared statement. "In every single case, green tea enhanced the bacteria-killing activity of the antibiotics. For example, the killing effect of chloramphenicol was 99.99 percent better when taken with green tea than when taken on its own in some circumstances." Kaseem and colleagues also found that green t...
Chloramphenicol in Biological News

Green tea helps beat superbugs

...ndria University in Egypt. In every single case green tea enhanced the bacteria-killing activity of the antibiotics. For example the killing effect of chloramphenicol was 99.99% better when taken with green tea than when taken on its own in some circumstances. Green tea also made 20% of drug-resistant bacteria su...

Microbes compete with animals for food by making it stink

... two different pools for two days -- one group in seawater where microbes were allowed to grow naturally and the other in seawater with the antibiotic chloramphenicol added to suppress microbe growth. In the lab, stone crabs readily ate both freshly thawed menhaden and fish that had soaked in water with antibiotics,...
Chloramphenicol in Biological Technology

Analysis Of Chloramphenicol by Negative Ion Electrospray LC/MS/MS

Z. Yang, Varian, Inc. Introduction Chloramphenicol (CAP) is a potent, broad-spectrum antibiotic and potential carcinogen used in humans only at therapeutic doses for treatment of serious infections. Its use in meatproducing animals, food producing insects, aquacultu...

Determination of the Chloramphenicol residues in milk and milk products using LC/MS/MS

Purpose The European Union prohibits the use of chloramphenicol (CAP) as a veterinary drug for food producing animals. For this reason a rapid and sensitive confirmatory method for the identification and quantification of CAP in milk and milk products has been developed using...

Simultaneously characterizing and quantifying chloramphenicol and its metabolite using LC/MS/MS

With the advent of liquid chromatography/ mass spectrometry and information dependent data acquisition function in mass spectrometry, it has become possible to characterise metabolites while to quantifying their parents during metabolic stability studies. This was once impossible to be an...

Transgenic Zebrafish by Electroporation

...ad. Sci. USA, 79 , 6777-6781, 1982). This plasmid uses the Rous sarcoma virus (RSV) promoter to effectively drive expression of chloramphenicol acetyl transferase (CAT) in numerous eukaryotic cell types. Methods Fish Maintenance and Egg Collection...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

...r 4.1-CMV vectors to drive high levels of mRNA and protein expression, vectors containing either a firefly luciferase cDNA or a chloramphenicol acetyltransferase (CAT) cDNA were prepared and transfected into HeLa cells. The close correlation between luciferase or CAT gene...

Transfection Reagent Selector Kit Handbook

... chloramphenicol acetyltransferase ...ommonly-Used Reporters chloramphenicol acetyltransferase The prokaryotic enzyme chloramphenicol acetyltransferase (CAT) is commonly used ...

TransMessenger Transfection Reagent Handbook

...ransfection, optimal expression levels may be obtained earlier following transfection with RNA. Cells transfected with a chloramphenicol acetyltransferase (CAT) reporter RNA are typically incubated for approximately 24 h post-transfection to obtain maximal ...

Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins

... performed in parallel using Ni-NTA Superflow Columns. For E. coli culture volumes and yields see Table 1. CAT: 6xHis-tagged chloramphenicol acetyl-transferase; TNF- alpha: 6xHis-tagged tumor necrosis factor- alpha; 46: 6xHis-tagged 46 kDa protein; 38: 6xHistag...

Lactococcus lactis

... Add 1 ml ice-cold complex medium, incubate 2 hours at 30 C. Plate diluted cells on selective chloramphenicol plates. Incubate 2 days at 30 C. Expected Results: Transformation...

Lactococcus lactis

... Add 1 ml ice-cold complex medium, incubate 2 hours at 30 C. Plate diluted cells on selective chloramphenicol plates. Incubate 2 days at 30 C. Expected Results: Transformation...
Chloramphenicol in Biological Products

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description: The loxP-cm-loxP cassette is designed to allow chloramphenicol selection in prokaryotic cells. The prokaryotic promoter gb2 driving the gene for chloramphenicol resistance is a slightly modified version of the Em7 promoter. It mediates higher transcription efficiency than the generally used Tn5 ...
Company:Gene Bridges GmbH

Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated, Clone CAT-1 from Abcam

Description:... Mouse monoclonal [CAT-1] to chloramphenicol Acetyltransferase ( Abpromise for all tested applications)....
Company:Abcam

Mouse Anti-Chloramphenicol Acetyltransferase Monoclonal Antibody, Unconjugated from Novus Biologicals

Description:... Mouse monoclonal [CAT-2] to chloramphenicol Acetyltransferase...
Company:Novus Biologicals

Luciferase Reporter Assay Kit from MBL International

Description:... a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any kn...
Company:MBL International
Chloramphenicol in Biological Definition

Antibiotic

...acin (Norflox) Levofloxacin (Levaquin) Trovafloxacin (Trovan) Streptogramins Sulfonamides Other important antibiotics: chloramphenicol Clindamycin Fusidic acid Trimethoprim Another such classification is by their mechanism of action (that is, the mechanism by...
Chloramphenicol in Biological Dictionary

Intergenic region

...ns of cloned mice ... variation of H3K9Ac enrichment in intergenic regions is the most prominent in ... ... intergenic region fused to a chloramphenicol acetyltransferase (CAT) reporter ... However, the common 740 bp intergenic region showed promoter activity in DDX13 ... ... a way in which i...

CAT assay

... Assay System provides two methods for monitoring chloramphenicol acetyltransferase activity. ... two alternative me...>Bu-14C-Cm Km (Cm) 10 µM ... CAT Assay - chloramphenicol AcetylTransferase ... To perform the enzyme ass... CAT in a fluid involves the use of a complex of chloramphenicol with a member of a specific ... A direct assay...
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