LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)

...nimal cells. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium homodimer (EthD-1), to st...tous cytoplasmic esterases. This releases the free calcein fluorophore that is retained inside live cells. Th... S. A new microcellular cytotoxicity test based on calcein AM release. Human Immunology 37: 264-270 (1993). ...

An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis

...aining 0.1% BSA. After 16-18h, the media was discarded and cells were washed twice with Hanks Balanced Salt Solution (HBSS) and stained with calcein AM (Invitrogen, C-3100MP), 8 μg/mL for 30 minutes at 37C/ 5% CO 2 . The dye was discarded and cells were washed twice to remove excess dye. Some...

Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection

...s transfected with Cy5- labeled siRNA, Cy5 positive cells were detectable after 4 hours (figure 1). Transfection efficiency measurements after calcein staining showed that over 80 % of the live cells were Cy5-positive. This picture remained constant after 24 hours. In cells transfected with ...

Detection of antibody-stained cell surface and intracellular protein targets with the Agilent 2100 bioanalyzer

...gure 1 shows representative histograms of a mixture of calcein-only and calcein and antibody treated cells. The data obtained with the 2100 bioanalyzer...nce in dye loading buffer after harvesting. 2. Stain cells with calcein (up to 5 x 106/ml, 0.5 M calcein, 15 minutes, 37 C) in dye loading bu...

Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer

...lectrically neutral or nearly neutral molecules, calcein acetyloxymethyl (AM) esters freely diffuse int...s that show strong annxin-V binding and a strong calcein signal, indicating an intact cell membrane, ar... 6. Add 1 l Cy5-streptavidin and 0.5 l calcein (calcein stock = 500 M, final = 0.5 M) per 500...