Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
...(GlaxoWellcome Research, U.K.)
3. Coelenterazine, both native and h iso forms from Molecular Probes, Inc.; http://www.probes.com
4. UTP, calbiochem
Cat. No. 6701; Tel: 1-800-492-1110
5. Hams F-12 Cat. No. 9058, Fetal Bovine Serum Cat. No. 3000, Glutamine Pen-Strep Solution Cat. No. 9316, G4...
Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
...MC substrate was purchased as part of Molecular Probes EnzChek Caspase3 Assay Kit #1 (catalog #E13183). Recombinant caspase3 enzyme was purchased from calbiochem
(catalog #235417), and 7Amino4Methylcoumarin (AMC) from Sigma (catalog #A 9891). Black 96-stripwell plates and white-96 well plates were purchased fro...
Measurement of proteasome inhibition in live cells in Molecular Devices microplate fluorometers
HEK 293 ZsGreen Proteasome Sensor Cell Line (BD Biosciences Clontech, Cat. #631535)
Acetyl-leu-leu-norleu-al (ALLN, calbiochem
Black-Wall Clear-Bottom 96-Well Microplate (Costar Cat. #3603)
Gemini EM Scanning Microplate Fluorometer (Mo...