Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

... 9410-PC (includes ImageQuant™ TL software) PlusOne™ bromophenol Blue 17-1329-01 PlusOne DTT ...

Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis

...from ammonia-oxidizing bacteria belonging to the genus Nitrosospira. 2,3 Approximately 200 ng of PCR product were mixed with 2x bromophenol blue loading dye and electrophoresed in a 6% polyacrylamide gel (acrylamide/bis 37.5:1) containing 7 M urea, 20% formamide, 2% g...

Heteroduplex Analysis Using the DCode System

...chamber in the DCode system. 5 l (200300 ng) of each amplified sample was mixed with 5 l 2x gel loading dye (70% glycerol, 0.05% bromophenol blue, 0.05% xylene cyanol, 2 mM EDTA) and electrophoresed on the DCode system at 100 V for 16 hours at room temperature. After e...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...ery other fraction was mixed with 40 l of sample buffer (125 mM Tris-HCl (6.8), 30% glycerol, 4% β-mercaptoethanol, 4% SDS, and 0.001% bromophenol blue). After being electrophoresed on 7.5% polyacrylamide gels, separated proteins were electrophoretically blotted to PVDF memb...

Use of the PROTEAN Plus Dodeca Cell for Second-Dimension SDS-PAGE, Rev A

...l of 50 mM Tris-HCl, pH 8.8, 6 M urea, 30% glycerol, 2% SDS, and bromophenol Blue (trace), first for 15 min with 100 mg dithioerythritol (DTE... Voltage was applied at 50 V for 0.5 hr, then at 200 V until the bromophenol Blue line reached the bottom of the gels (about 6 hr). The Dodec...

Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing

...d of Bradford 29 at 595 nm. For preparative electrophoresis (PE) 0.0625 M Tris-HCl (100 l), pH 6.8, containing 25% glycerol and bromophenol blue 0.012% (final concentration) were added to the βLG solution. STANDARDS βLG variants A, B and A/B fro...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

...to a 12% cylindrical SDS-PAGE gel and electrophoresed as indicated in Table 1. Collection of 2 ml fractions was started when the bromophenol Blue band passed the gel matrix. Following electrophoresis, 200 l aliquots of every fifth eluted fraction were TCA-prec...

Preparative Nondenaturing Gel Electrophoresis to Purify NADP-Specific Glutamate Dehydrogenase From Chlorella, Rev B

... min and was combined with 3 ml of 40% sucrose and 1 ml of 0.02% bromophenol Blue. Preparative Nondenaturing Gel Electrophoresis ... and 6 ml fractions were collected. The first fraction after the bromophenol Blue marker eluted, fraction 1, was collected after 2 hr of elec...

Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

...ere mixed in a 25 l volume and the total sample volume made up to 100 l. We added 100 ml 2x gel loading dye (70% glycerol, 0.05% bromophenol blue, 0.05% xylene cyanol, 2 mM EDTA) to these samples, and electrophoresed them on the DCode system at 130 V for 2.5 hours at 5...

Detection of Platinum Species in Plant Material by Preparative Isotachophoresis Using the Model 491 Prep Cell, Rev A

...le volume, pH 8.0, + 0.1 ml carrier ampholyte, pH 5 7, + 0.1 ml carrier ampholyte, pH 6 8, + 0.1 ml carrier ampholyte, pH 7 9). bromophenol blue was used as a marker. The sample was carefully loaded on the surface of the stacking gel. The operating conditions ...

Bromophenol in Biological Products

Electrophoresis Loading Dye, 6X from Research Organics, Inc.

Description:... Each bottle contains: bromophenol Blue 0.25% w/v , Xylene Cyanol FF 0.25%, Glycerol solution 30% w/v...
Company:Research Organics, Inc.

Gel Loading Dye (Bromophenol Blue/Glycerol) from Mo Bio Laboratories, Inc.

Description:... Makes gels easy to load and track during electrophoresis. 10X concentrate of bromophenol Blue, Xylene Cyanol, glycerol solution in TE...
Company:Mo Bio Laboratories, Inc.

Gel Loading Dye (Bromophenol Blue/Glycerol) from Mo Bio Laboratories, Inc.

Description:... Makes gels easy to load and track during electrophoresis. 10X concentrate of bromophenol Blue, glycerol solution....
Company:Mo Bio Laboratories, Inc.

Gel Loading Dye (Bromophenol Blue, Xylene Cyanol/Glycerol) from Mo Bio Laboratories, Inc.

Description:... Makes gels easy to load and track during electrophoresis. 10X concentrate of bromophenol Blue, Xylene Cyanol, glycerol solution in TE...
Company:Mo Bio Laboratories, Inc.

BioTracker Dye 1ml from BioVentures

Description:... "BioTracker 6X bromophenol Blue Tracking Dye is composed of 60% St DI H2O, 30% Glycerol, 10% 10XTE, 0.25% BPB Dye and 0.85% SDS....
Company:BioVentures

Gel Loading Solution from Sigma-Aldrich

Description:...uffer is especially formulated for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids. The gel loading solution contains a bromophenol blue tracking dye and sucrose to add density and facilitate sample loading. EDTA has been included to inhibit nucleases that require divalent cations ...
Company:Sigma-Aldrich

Orange G Loading Buffer from MBL International

Description:... Orange G Dye runs faster than bromophenol blue or methylene blue dyes in standard agarose gels. Orange G dye migrates with DNA between 10 and 20 nucleotides long. The migration at this size pr...
Company:MBL International

PROTEAN Plus Overlay Agarose, 125 ml from Bio-Rad

Description:... SDS-PAGE sample setup to secure IPG strips in place in PROTEAN Plus cells. bromophenol Blue tracking dye is incorporated to allow monitoring of electrophoresis ru... solution is composed of 0.75% agarose in 1x Tris, glycine, SDS, and 0.003% bromophenol Blue and is supplied as 125 ml....
Company:Bio-Rad

ReadyPrep Overlay Agarose, 50 ml from Bio-Rad

Description:... SDS-PAGE sample setup to secure IPG strips in place for most applications. bromophenol Blue tracking dye is incorporated to allow monitoring of electrophoresis ru...osed of 0.5% low melting point agarose in 1x Tris, glycine, SDS, and 0.003% bromophenol Blue and is supplied as 50 ml....
Company:Bio-Rad

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