Quantitative Measurement of Cell Proliferation
Using the BrdU ELISA: A Comparison
Between Colorimetric and Chemiluminescent Detection
Quantitative determination of DNA synthesis in cell
cultures is now a routine procedure in many laboratories.
Protocols are available for various applications,
especially in cell culture systems. The effects of growth
Quantitative Analysis of the Cell Cycle in Imaging Applications
...t had synthesized DNA. 2, 3, 4, 5 In this method, brdu
(an analog of the DNA precursor thymidine) is inco...g treatment, cells were cocultured with 20 μM brdu
(Calbiochem, 203806) (a thymidine analogue that in... threshold was set on the nuclear intensity of the brdu
and phospho-Histone 3 negative cells.
Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
...nthesis) phase of the cell cycle. The incorporated brdu
is detected with a specific anti-BrdU monoclonal a...oglobulin, giving a fluorescent signal at sites of brdu
incorporation. This provides an excellent marker f...
> Day 2.
labeling reagent 1:250 with Growth medium and add ...
Cell Proliferation and Viability Measurement
...g amounts of actinomycin D for
20 hours. After labeling of the cells with brdu
2 hours, Cell Proliferation Reagent WST-1 was added,
and the cells ...,
BrdU incorporation was determined using the Cell
Proliferation ELISA, brdu
(colorimetric). The results
show that Actinomycin D inhibits DNA synthesi...
Roche Diagnostics Corporation
Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus
Quantitative Measurement of Cell Proliferation Using the brdu
RNase Protection Assay (RPA) Using DI...