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BRDU in Biological Technology

Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection

Quantitative determination of DNA synthesis in cell cultures is now a routine procedure in many laboratories. Protocols are available for various applications, especially in cell culture systems. The effects of growth f...

Quantitative Analysis of the Cell Cycle in Imaging Applications

...t had synthesized DNA. 2, 3, 4, 5 In this method, brdu (an analog of the DNA precursor thymidine) is inco...g treatment, cells were cocultured with 20 μM brdu (Calbiochem, 203806) (a thymidine analogue that in... threshold was set on the nuclear intensity of the brdu and phospho-Histone 3 negative cells. ...

Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

...nthesis) phase of the cell cycle. The incorporated brdu is detected with a specific anti-BrdU monoclonal a...oglobulin, giving a fluorescent signal at sites of brdu incorporation. This provides an excellent marker f... > Day 2. 3. Dilute brdu labeling reagent 1:250 with Growth medium and add ...

Cell Proliferation and Viability Measurement

...g amounts of actinomycin D for 20 hours. After labeling of the cells with brdu for 2 hours, Cell Proliferation Reagent WST-1 was added, and the cells ..., BrdU incorporation was determined using the Cell Proliferation ELISA, brdu (colorimetric). The results show that Actinomycin D inhibits DNA synthesi...

Roche Diagnostics Corporation

... Quantification of Nucleosomes in Serum by the Cell Death Detection ELISAplus Quantitative Measurement of Cell Proliferation Using the brdu ELISA RNase Protection Assay (RPA) Using DI...
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