Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Andreas Henke and Eunike Grohmann, Institute of Virology, Medical Center,Friedrich Schiller University Jena, Winzerlaer Str. 10, 07745 Jena, Germany The Helios gene gun-mediated DNA transfer can be usedas a fast and efficient method to transfect living cells. Inthis study we describe the preparation of DNA-coatedmicroc...
Transformation of Filamentous Fungi by Microprojectile Bombardment
Roland W. Herzog, Molecular Genetics Program, Department ofBotany and Microbiology, 101 Life Sciences Building, AuburnUniversity, AL 36849-5407, USA The vast majority of transformation protocols for filamentous fungi are based on permeabilizing cell membranes with polyethylene glycol (PEG) or...