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Blots in Medical News

Carestream Molecular Imaging Releases Western Blot Kits For Added Convenience, Better Performance

...enient, cost-effective kits for performing western blots -- a popular analytical technique used to detect p...nient, cost-effective kits for performing western blots -- a popular analytical technique used to detect ...tiplex applications . Each kit is optimized for 10 blots and includes best-in-class membranes and buffers t...

First Bacterial Genome Transplantation Changing One Species to Another

...dictated by the transplanted chromosome not the recipient cell chromosome. The new, transformed organisms show up as bright blue colonies in images of blots probed with M. mycoides LC specific antibody. The group chose to work with these species of mycoplasmas for several reasons -- the small genome...
Blots in Medical Technology

The Past, Present and Future of HLA Typing

...ligonucleotide probe. The original methods, described in the 1980s, first blotted PCR amplified DNA onto nitrocellulose or nylon filters. These dot blots could then be incubated with radioactively labeled, sequence specific oligonucleotide probes. These probes would bind only to complementary HLA DNA...
Blots in Medical Products

ROYAL GENIE BLOTTER. (Blots gels up to 20 x 20 cm.

Description:...result is exceptionally rapid and exact electrophoretic transfer. blots gels up to 20 x 20 cm. Uses 1.4 liters of buffer. Platinized titanium anode....
Company:Idea Scientific

Antigen-Antibody Pen For Hamster Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Rabbit Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Sheep Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Mouse Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For G. Pig Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Goat Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Human Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Chicken Primary antibodies

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.

Antigen-Antibody Pen For Biotin-tagged antibodies (all species)

Description:...ally formulated antigen-bonded colored dyes helps to see antigen being written on the membrane. One can mark the size of the markers or the lanes. The blots are processed as usual with the appropriate 2-ab antibody conjugates, and ECL: results recorded on films or digital imager. Unlike the situation in Fi...
Company:Alpha Diagnostic International, Inc.
Blots in Medical Dictionary

Total protein

...) provided with the ... The contribution of fibrinogen to total protein content in plasma compared to serum. ... Total protein Western blots for proteomics, gene discovery, & protein functional ... Protein Kits. Peptides and Blocking Peptides. Recombinant Proteins. Total ... ...
Blots in Biological News

Spelling out cancer on the nanoscale

...hat MYC was overexpressed in Burkitt’s, while BCL2 was overexpressed in mantle cell and follicular lymphoma patients. In parallel, traditional Western blots were performed to confirm BCL-2 and MYC levels. "Our strategy can be used to repetitively and quickly assess the levels of oncoproteins in canc...

Researchers discover which organs in Antarctic fish produce antifreeze

...NA in the liver, but this was inconsistent with high levels of production of the protein, the researchers noted. Cheng and colleagues used Northern blots of total RNA from various tissues to hybridize with an AFGP gene probe. A clear picture of strong AFGP mRNA expression came into focus in the pancreat...

Nonlinear Dynamics announces more details of its global partnership with PerkinElmer

...re as the centrepieces to that strategy. Our intention is to reaffirm PerkinElmer as the first choice in gel imaging, whether it be for 1D or 2D gels, blots or any other applications." John Spreadbury, Group Sales and Marketing Director of Nonlinear Dynamics and CEO of Nonlinear USA Inc., added, "Nonlin...
Blots in Biological Technology

Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer

Matthew W. Fields, Section of Microbiology, Cornell University, Ithaca, New York 14853 Introduction Biologists frequently monitor the effect of environmental stimuli on living organisms by quantitating mRNA levels. The relative abunda...

Imaging Chemiluminescent Western Blots

Use the Eagle Eye II still video system to directly acquire images from chemiluminescent blots Michelle Cayouette Jane Moores Stratagene The Eagle Eye II still video system is well suited for direct imaging of chemiluminescent Western blots. The light-tight cabinet is idea...

Technical Note: E-PAGE(TM) 48- and 96-Well Gels on the Odyssey(R) Infrared Imaging System

... for E-PAGE gel transfer, such as the Trans-Blot(R) Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories, Hercules, CA). Detection for blots processed in this manner can be performed using IRDye(R) Infrared Secondary Antibodies and the Odyssey Infrared Imaging System. IRDye antibody conjuga...

Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter

...t it provides the ability to measure 32 P labels directly in the microplate without cocktail. This makes stripping and reprobing of 32 P labeled dot blots possible after counting. In addition, Cerenkov counting provides the ability to perform other molecular biology applications such as PCR product scree...

Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager

...tion overnight at 4 °C.

The blots were then incubated with the rabbit anti–hum...

After the blocking step, the blots were incubated in a mixture of rabbit anti–h... h at room temperature. After washing in PBST, the blots were incubated with a mixture of the secondary ant...

Transgenic Zebrafish by Electroporation

...ation solution. Hybridization takes place overnight at 68 C. Dot blots are washed the following morning, covered with plastic wrap, and... 1987). Nontreated fish were used as controls. Southern blots are in progress at the time of this writing to determine integra...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

...fter heat shock (Wippersteg et al. 2002). Protein from larval stages was extracted 48 hr after bombardment. SDS-PAGE and western blots were done according to standard protocols. Microscopic analyses of bombarded worms were done 24 hr after heat shock. A...

A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF

...ectrophoretically blotted to PVDF membranes (5.5 x 8.5 cm) at a constant current of 2.0 mA/ cm 2 of gel surface for 30 minutes. blots were either stained with 0.05% Coomassie Blue or probed with antibody developed to tobacco BiP fusion protein (TOBBLP4, gift from Dr. J. De...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

...owing 2-D PAGE and transfer of separated proteins to a nitrocellulose membrane, biotin-labeled proteins were detected by probing blots with ExtrAvidin-alkaline phosphatase (Sigma) and visualized colorimetrically by the addition of NBT/BCIP. IEF and Sec...

Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C

...d and nonspecific binding. Immun-Blot PVDF membrane is ideal for chemiluminescent (Figure 1) and colorimetric (Figure 2) western blots because it very strongly retains target protein (140150 g protein/cm 2 membrane) but resists background that can obscure highsensitivity d...
Blots in Biological Products

Total Protein Western Blots - Plant Normal Tissue, Blot I from BioChain

Description: Biochain's plant total protein western blot (WtPAN) is designed for rapidly screening tissue specific protein expression in plant tissues. Human placenta total protein is included in each blot as an interblot control for comparison between different western blots. Each lane contains 50 g of protein...
Company:BioChain

Disposable Staining Tray for Western Blots from PGC Scientifics

Description: • Small design specifically for western blots • Optically clear plastic construction Trays are specifically designed for staining western blots. The reduced size (60 x 75 x 25 mm) of the disposable tray requires less dye and antibodies resulting in a significant cost savings to the...
Company:PGC Scientifics

Blot Processing Standard Tray (3 per pack) from GE Healthcare, formerly Amersham Biosciences

Description:... system for gel staining and blot processing.Automated staining of Coomassie Blue or silver-stain gels for proteins and nucleic acid analysis.Prepares blots for high sensitivity ECL detection and chromogenic detection.Programmable control of protocol, solution, volume, and processing time.Proven design for...
Company:GE Healthcare, formerly Amersham Biosciences

Blot Processing mini Tray (3 per pack) from GE Healthcare, formerly Amersham Biosciences

Description:... system for gel staining and blot processing.Automated staining of Coomassie Blue or silver-stain gels for proteins and nucleic acid analysis.Prepares blots for high sensitivity ECL detection and chromogenic detection.Programmable control of protocol, solution, volume, and processing time.Proven design for...
Company:GE Healthcare, formerly Amersham Biosciences

Paraffin Tissue Section Panel - Monkey (Simian) Normal Tissue, Multi-tissue I from BioChain

Description:... localization of genes or proteins of interest in different monkey tissues. Tissues are mounted on positively charged glass slides. Total RNA Northern blots are available in the identical format, and cDNA from those tissues may also be available for PCR detection....
Company:BioChain

Stainless Steel Staining tray 19 x 29 cm from GE Healthcare, formerly Amersham Biosciences

Description:...system for gel staining and blot processing.Automated staining of Coomassie Blue or silver-stain gels for proteins and nucleic acid analysis. Prepares blots for high sensitivity ECL detection and chromogenic detection. Programmable control of protocol, solution, volume, and processing time.Proven design fo...
Company:GE Healthcare, formerly Amersham Biosciences

Rabbit Anti-mTOR Monoclonal Antibody,, Clone ZMD.414 from Invitrogen

Description:...al region of human mouse and rat mTOR (Mammalian target of rapamycin FRAP RAFT1 RAPT1). Specificity: Specific for the human mTOR protein. On Western blots it identifies the target band at ~235 kDa. Reactivity: Human (positive control: HEK293 cell lysates). Based on sequence homology reactivity with mous...
Company:Invitrogen

Mouse Anti-Human MUC5B (Mid) Monoclonal Antibody,, Clone 5B15-5B-11E from Invitrogen

Description:... peptide derived from the internal region of the human MUC5B protein. Specificity: Specific for the internal region of the MUC5B protein. On Western blots it identifies the target band at ~590 kDa. Reactivity: Human (positive controls: deglycosylated secreted media from primary tracheobranchial epithel...
Company:Invitrogen

Custom Monoclonal Antibody Development from A&G Pharmaceutical, Inc.

Description:...ation strategy. Immunization of mice. Serum screening by EIA and/or WB if blots are provided by the customer. E-mail data to customer for evaluation and f...ning and screening by EIA. Screening up to 20 EIA-positive clones by WB if blots are provided by the customer. Inquire about other available screening metho...
Company:A&G Pharmaceutical, Inc.

Rabbit Anti-Human C35 Monoclonal Antibody,, Clone ZMD.460 from Invitrogen

Description:... region of the human C35 protein (chromosome 17 open reading frame 37 (C17orf37 protein) XTP4. Specificity: Specific for the C35 protein. On Western blots it identifies the target band at ~15 kDa band. Reactivity: Human (positive control: full-length human C35-transfected HEK293-(DYKDDDK or DYKDDDDK) ce...
Company:Invitrogen
Blots in Biological Definition

His-tag

...tion The his tag can also be used in detection of the protein via anti-his-tag antibodies . This can be useful in localization , ELISA , western blots or other immunoanalytical methods. ...
Blots in Biological Dictionary

Western blotting

...onal antibodies can also be used for Western blots . ... Northern and then Western blots were named by analogy. Blotting techniques firs...ern blot (more formally called a ... Western blots allow investigators to determine the molecular we...

Western blot

...y and add a piece of filter paper slightly ... Northern and then Western blots were named by analogy. ... A Western blot is a method in molecular ...cedure ... of how a western blot (more formally called a ... Western blots allow investigators to determine the molecular weight of a protein ... ...

Thylakoids

...rn analysis of thylakoids from PS1 mutants. ... the 1500 to 1800 cm-1 frequency range for the thylakoids of WT and PS1 mutants. ... ... Western blots of mutants and normal thylakoids (antisera were kindly provided ... thylakoids , we believe the new polypeptides in the mutant are related to ... ...

Southern blotting

...ected by southern blotting . ... Southern blots analysis of transgenic mice need copy standards to estimate copy number. ... Southern blots are commonly used to determine transgene copy num... Southern blotting ... Analysis of Southern blots . Here's a simple example. ... You perform a Sout...

Southern blot

... Southern blotting ... Analysis of Southern blots . Here's a simple example. ... You perform a Sout...n of this process. ... Worm genomic Southern blots . by Michael Koelle. 4/6/94 ... Later, will use th...y adding a lane containing the ... " Southern blots " is a short-hand term for a rather lengthy proced...

Northern hybridization

... University of ... ... the quantification of DIG-labeled RNA and as a hybridization . control ... Note: Hybridization temperature for Northern blots with DIG Easy Hyb is 68 °C (for ... Northern Hybridization Solution. AB10055. DEPC treated. Description ... Northern Hybridization Solution...

Northern blot

... Kits ... However, a description of Northern blots is included here in the interest of ... It is mRNA which is isolated and hybridized in northern blots . ... Learn about the northern blot techniq...nd reprobed a dozen or ... When making Northern blots , however, it is preferable to visualize the RNA ....

Northern blotting

... Kits ... However, a description of Northern blots is included here in the interest of ... It is mRNA which is isolated and hybridized in northern blots . ... Northern blotting is in principle the...nd reprobed a dozen or ... When making Northern blots , however, it is preferable to visualize the RNA ....
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