Bisulfite

New method developed by UC San Diego bioengineers gives regenerative medicine a boost

...c targets using padlock probes. Zhang and his group describe the breakthrough in a recent paper published in Nature Biotechnology titled "Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming." DNA methylation is a type of chemical modification of DNA tha...

New nucleotide could revolutionize epigenetics

...n most epigenetic experiments. Typically, scientists use a procedure called bisulfite sequencing to identify the sites of DNA methylation. But this test cannot d...example, is in the process of mapping all of the sites of methylation using bisulfite sequencing. "If it turns out in the future that (5-hydroxymethylcytosine an...

Broad Institute awarded major grant to bolster epigenomics research

...n switched off. In the past few years, two techniques have transformed researchers' abilities to probe cells' epigenomes: ChIP-Seq and high-throughput bisulfite sequencing (HTBS). These technologies can help pinpoint the genomic locations of various types of chemical tags, such as methyl groups, and thus chart...

New decontamination system kills anthrax rapidly without lingering effects

...ith harsh liquid chlorine dioxide. In addition, people cannot re-enter a room fumigated with chlorine dioxide until the gas is neutralized with sodium bisulfite vapor and vented from the building. The new decontamination system resembles a coat rack with radiation modules arranged on rings at various height...

Bisulfite in Biological Products

EpiTect Bisulfite Kit from QIAGEN

Description: The EpiTect Bisulfite Kit enables complete conversion of unmethylated cytosines to uracils in less than 6 hours. The highly sensitive method utilizes an innovative protection against DNA degradation and a spin-column-based purification and desulfonation procedure to guarantee fast and reliable resu...
Company:QIAGEN

CpG WIZ® DAP-KINASE AMPLIFICATION KIT from CHEMICON

Description:...mounts of DNA (5). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplif...ains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® DAP-kinase Ampli...es by MSP. However, the samples must first undergo bisulfite modification prior to PCR amplification. The CpGen...
Company:CHEMICON

CpGenome™ Fast DNA Modification Kit from CHEMICON

Description:...cation Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminate...) designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will be generat...
Company:CHEMICON

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:...it uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is ba...ction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The EZ DN...t: Chemical denaturation of DNA followed by sodium bisulfite modification, Spin Column. *Recovery Volume: Pur...
Company:Zymo Research

EZ DNA Methylation Kit from Zymo Research

Description:... The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The EZ DNA Methylation Kits innova...
Company:Zymo Research

EZ-96 DNA Methylation Kit from Zymo Research

Description:... The EZ DNA Methylation Kit uses a simplified procedure and streamlines the bisulfite method for DNA methylation analysis. The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite where cytosine is converted into uracil. The EZ DNA Methylation Kits innova...
Company:Zymo Research

Bisulfite in Biological Dictionary

DNA methylation

... such, it is part of the epigenetic code and is also the best characterized epigenetic mechanism. Because methylation ... ... is the use of bisulfite treatment of DNA to determine its pattern of methylation . DNA methylation was the first discovered epigenetic mark, and remains the ...