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Biolistic in Biological Technology

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

Barbara Randolph-Anderson 1 , John E. Boynton 1 , John Dawson 2 , Erik Dunder 2 , Robert Eskes 3 , Nicholas W. Gillham 1 , Anita Johnson 1 , Philip S. Perlman 3 , Jan Suttie 2 , and William C. Heiser 4 . 1 Developmental, Cell and Molecular Biology Gro...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

William Heiser, Ph.D., Research and Development Department, Genetic Systems Division, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547 Summary We have found that the most important factors which must be optimized in Biolistic transf...

Comparison of Performance Characteristics of Different Biolistic Devices

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Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System

Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab, USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705 Introduction In 1987 Jefferson et al. described the use of β-glucuronidase (GUS) as a marker to ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

...rown in culture, such as obligate plant pathogens. biolistic transformation of Saccharomyces cerev... bombardment were compared with literature data on biolistic transformation of other fungal species.... et al ., 1983; Waring et al ., 1989). biolistic transformation Intact conidia were sp...

Transformation of Nematodes With the Helios Gene Gun

... Introduction The biolistic DNA transfer technique was first established for...legans hermaphrodites are killed during biolistic DNA transfer, the survivors with their gold partic...n efficiency has been observed for this biolistic DNA transfer method as for microinjection ...

Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System

...e the use of the Hepta adaptor for the PDS-1000/He biolistic system for transformation and transient...the Hepta adaptor reduces the number of biolistic transfers necessary to obtain sufficien...ide in a single 9 cm petri dish for the biolistic transfer. After evacuating the shocking chamber ...

Biolistic Transfection of Organotypic Brain Slices

... shooting biological materials into living tissue. biolistic transformation, which involves accelera...rs since the method was first reported, biolistic bombardment has also been proven effective in tran...ain slice cultures. We have applied the biolistic technology to transfect these preparations ...

Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

...e with a complex life cycle. Specifically, we used biolistic gene transfer techniques to transiently...al stages of the schistosome. biolistic gene transfer, also known as particle bombardment,...t shock was applied for 4 hr at 42C. For biolistic experiments with sporocysts, groups of 2,000 4,000...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

... References Armaleo, D., et al., biolistic nuclear transformation of Saccharomyces cerevisiae and other fun...t Microbe Interact., 12 , 1074-1081 (1999) Shark, K. B., et al., biolistic transformation of a procaryote, Bacillus megaterium, Appl. Envir...

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Biolistic in Biological Products

Helios Gene Gun Low-Pressure System, 100-120 V from Bio-Rad

Description:... The Helios gene gun low-pressure system, 100-120 V, is used for biolistic particle delivery of biomaterials into cells. The system includes the Helios gene gun, helium hose assembly, helium regulator, tubing prep station, sy...
Company:Bio-Rad

500 Optimization Kit, PDS-1000 He and Hepta systems from Bio-Rad

Description:...mization kit is for fine-tuning the bombardment conditions for your cells of interest. The kit is recommended when you are first using the PDS-1000 He biolistic system. The kit includes 0.25 g each of 0.6 micrometer, 1.0 micrometer, and 1.6 micrometer diameter gold microcarriers, 100 each of 9 rupture disks, 5...
Company:Bio-Rad

Stopping Screens for PDS-1000 He and Hepta Systems, 500 from Bio-Rad

Description:... These stopping screens are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 screens....
Company:Bio-Rad

Macrocarriers, for PDS-1000 He and Hepta systems, 500 from Bio-Rad

Description:... These macrocarriers are for the biolistic PDS-1000 He and Hepta systems. Supplied as 500 macrocarriers....
Company:Bio-Rad

Helios Gene Gun System, 100-120 V from Bio-Rad

Description:... The Helios gene gun system, 100-120 V, is used for biolistic particle delivery of biomaterials into cells. This handheld device employs an adjustable helium pulse to sweep DNA-, RNA-, and other biomaterial-coate...
Company:Bio-Rad

Helios Gene Gun Low-Pressure System, 220-240 V from Bio-Rad

Description:... The Helios gene gun low-pressure system, 220-240 V, is used for biolistic particle delivery of biomaterials into cells. This handheld device employs an adjustable helium pulse to sweep DNA-, RNA-, and other biomaterial-coate...
Company:Bio-Rad

Helios Gene Gun System, 220-240 V from Bio-Rad

Description:... The Helios gene gun system, 220-240 V, is used for biolistic particle delivery of biomaterials into cells. This handheld device employs an adjustable helium pulse to sweep DNA-, RNA-, and other biomaterial-coate...
Company:Bio-Rad

Hepta Adaptor for PDS-1000 He System from Bio-Rad

Description:... The Hepta adaptor for the PDS-1000 He biolistic system enables 7-10 times more cells to be transformed than the standard system. It fits into the shocking chamber of the PDS-1000 He system, where it...
Company:Bio-Rad

PDS-1000 He Hepta System from Bio-Rad

Description:... The PDS-1000 He Hepta system includes the PDS-1000 He biolistic system and the Hepta adaptor....
Company:Bio-Rad

PDS-1000 He System from Bio-Rad

Description:... The biolistic PDS-1000 He system uses a burst of high-pressure helium gas to accelerate micron-size nucleic acid-coated gold or tungsten microparticles to velocitie...
Company:Bio-Rad
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