Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators

Serial Analysis of Gene Expression (SAGE) is a sequence-based approach to the identification of differentially expressed genes by comparative analyses. SAGE is a powerful tool, one of the more comprehensive methods available for detailed analysis of large numbers of cellular transcripts, ...

Comparison of Commercial Dye Terminator Removal Kits, Rev A

...cturers were assessed using these four criteria (Table 1). Methods Cycle sequencing was performed using bigdye terminators, version 3.0 (Applied Biosystems). The plasmid template used was pGEM-3Zf(+), acquired from Promega. The primer used...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

...en was used for cloning. The agarose gel extraction kit and plasmid extraction kit were purchased from QIAGEN Ltd. The ABI PRISM bigdye terminator v 3.0 cycle sequencing kit with AmpliTaq DNA polymerase, FS was purchased from Applied Biosystems. The TaqMan reagent...

cDNA Sequencing & Finishing

... Data delivery via a secure FTP site SEQUENCING AND FINISHING Purified cDNA clones are sequenced using bigdye Terminator v3.1 chemistry and reactions purified using CleanSEQ, a SPRI-based dye-terminator removal system. Typical Phred20 rea...

CleanSEQ on the ABI 3100

... Experimental Methods pGEM was sequenced using v3.1 bigdye Terminator chemistry at 1/4x dilution. Sequencing reactions wer... CleanSEQ with water elution allow extensive dilution of bigdye Terminators without affecting Phred20 read lengths (data not show...

SPRINTPREP SINGLE STEP PLASMID PURIFICATION

...00 cDNA clones as part of the Mammalian Gene Collection. Purified plasmids were sequenced using 30ng of template DNA in a 1/16th bigdye v3.1 terminator reaction. Unincorporated dye-terminators were removed using CleanSEQ and sequences read on ABI 3700 and 3730xl's...

DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification

...llary sequencing (Figure 3). Using either bigdye terminator v.2.0 or v.3.0 sequencing chem... and reactions were sequenced using bigdye terminator v. 2.0 chemistry and ana... Kit. Plasmid pUC19 was sequenced using bigdye terminator v. 3.0 chemistry in a cy...