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Bgl in Biological Technology

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

...estriction enzyme recognition sites for bgl II, Pst I, and Xho I. ...re digested separately with Stratagenes bgl II, Pst I, and Xho I restriction enzyme...9 out of 12 randomly chosen clones. The bgl II, Pst I, and Xho I restriction digest...

Epitope-Tagging Vectors for Functional Analysis in Yeast

...mid backbone with a selectable marker, such as LEU2, HIS3, TRP1, or URA3.) For N-terminal tagging with FLAG, the luciferase gene was cloned in the bgl II site of the pESC vectors, resulting in pESC-XXX-LucFN constructs. The constructs were transformed into S. cerevisiae, strain YPH499, and the tr...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

... N-terminus bgl II Vector sup... C-termini bgl II and MCS 2 ...nal MCS 1 and bgl II Insert sup...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

...he AdEasy system described below. The pShuttle-CMV-GFP vector was prepared by cloning the sequence for green fluorescent protein (GFP) between the bgl II and Xho I sites into pShuttle-CMV vector. The pShuttle-CMV-LacZ vector was prepared by cloning the sequence for E. coli LacZ between the Xho I ...
Bgl in Biological Products

pAcAB4 Baculovirus Transfer Vector from BD Biosciences Pharmingen

Description:...am of this, an inverted polyhedrin promoter has been inserted containing Xba I and Stu I as single cloning sites, followed by a second p10 promoter, a bgl II insertion site and an SV40 termination sequence. Additionally, there is another polyhedrin promoter and BamH I cloning site downstream of the first...
Company:BD Biosciences Pharmingen

pAcHLT-A-BFP from BD Biosciences Pharmingen

Description:...essed as BFP-6xHis fusion proteins when cloned into one of the available restriction enzyme sites (EcoR I, Stu I, Sac I, Not I, Pst I, Kpn I, Sma I or bgl II). The presence of the BFP coding sequence allows the visualization of fusion proteins....
Company:BD Biosciences Pharmingen

Bgl I from USB Corp.

Description:... 10-fold over digestion with this enzyme, the DNA fragments are ligated with T4 DNA Ligase and re-digested with this enzyme. Includes (1 ml each): 10X bgl I Buffer and 10X Loading Buffer. A USB Floatboat will be sent free with every USB Restriction Enzyme. ...
Company:USB Corp.
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