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BamHI in Biological Technology

Transgenic Zebrafish by Electroporation

...y gradient ultracentrifugation. DNA is suspended in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) and 100 g are digested overnight with bamhi at 37 C. The digest is extracted once with phenol/chloroform and once with chloroform alone. The DNA is precipitated from the aq...

Transformation of Filamentous Fungi by High-Voltage Electroporation

... hybridization for three transformants. The pAN7-1 plasmid is about 6.5 kb in size, and it has no EcoRV sites, one site each for bamhi and HindIII, and two sites for EcoRI (which yields two fragments of about 3.9 and 2.6 kb). The digestions showed the restriction...

The iCycler iQ Detection System for TaqMan Assays, Rev B

...en 416 l aliquots were transferred into eight 2-ml skirted tubes. Human genomic DNA, 100 g/l (Clontech) was partially digested by bamhi at 37C for 2 hr, then heated to 100C for 5 min before being plunged into an ice-water bath. A 2-fold dilution series was prepare...

Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A

...a 66.7 ng/l stock to generate a 4-fold dilution series from 50 to 3.125 ng/l. The genomic DNA stock was previously digested with bamhi and boiled for 10 min before being placed in an ice bath. Aliquots of 423 l from either the 6-FAM/BHQ-1 or SYBR Green I master m...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

... Genomic DNA Multiplex Prior to performing PCR, human genomic DNA (250 ng/l; Promega) was partially digested with bamhi at 37C for 2 hr, and then heated to 100C for 10 min before immediately cooling on ice. This DNA was then stored at 4C until use....

Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice

... using the QIAGEN EndoFree Plasmid Mega kit. The presence of the HBsAg insert was verified by restriction enzyme digestion using bamhi and analysis by agarose gel electrophoresis. Preparation of DNA-Coated Gold Microcarriers On the day prior to vaccination, pCM...

Isolation of Cosmids using Eppendorf's Perfectprep Plasmid Mini Kit

...mid DNA at either +4C or 20C. Analysis Restriction digests were carried out to a final volume of 15 l using bamhi [4] and 7.5 l of each purified sample. The reactions were incubated at 37C for 1 hour. Electrophoretic analysis was carried out ...
BamHI in Biological Products

CopyRight pEZ BAC BamHI Cloning Kit (without cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (without cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen
BamHI in Biological Definition

Restriction enzyme

... 5'GAATTC 5'---G AATTC---3' 3'CTTAAG 3'---CTTAA G---5' bamhi Bacillus amyloliquefaciens 5'GGATCC 5'---G GATCC---3' 3'CCTAGG 3'---CCTAG G---5...
BamHI in Biological Dictionary

Rare- cutter enzyme

..... 11. Use 5 ul for digestion with Notl or other rare cutter enzymes . ... Use 7-10 ul for digestion with a more frequent cutter such as bamhi or EcoRI. ... ...

Pseudorevertant

...e of news and information on Photosynthesis Research ... In the pseudorevertant , up-regulated transcripts included stress-responsive ... A mutant bamhi endonuclease, E77K, belongs to a class of catalytic mutants that bind DNA efficiently but cleave DNA at a ... Both pseudorevertant proteins ... ...
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