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BamH in Biological Technology

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

... fragment encoding residues 208 to 313 of rat c-Fos 12 into the bamh I and EcoR I sites of pFA-CMV. Our results ( figure 3...no acid residues 1-96 of human ATF2 14,15 was inserted into the bamh I and EcoR I sites of the pFA-CMV vector. Consistent with previo...

High-Titer Retroviral Vectors for Gene Delivery

... Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the EcoR I and bamh I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) was used for...

A New Lambda Vector for Mammalian Expression

...ure 4 To demonstrate expression levels in the pCMV-Script-EX vector, we inserted the firefly luciferase gene into the bamh I site of the MCS. This same luciferase gene was inserted into the parental pCMV-Script vector. These two constructs were used f...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...ostatin]. GST-MEK1 CA was purified as described. 2, 10 For expression in E. coli, the entire coding sequence of MEK1 CA was inserted at the bamh I site of the pGEX-2T plasmid. Epicurian Coli XL1-Blue cells containing pGEX-MEK1 CA were induced by IPTG (1 mM) for 4 hours, when OD 600 reac...

Epitope-Tagging Vectors for Functional Analysis in Yeast

...AL1 or GAL10 promoter. To compare the promoter activity under repressed and induced conditions, the luciferase gene was individually cloned in the bamh I site or the Not I site of pESC-TRP, so that the gene is under the control of the GAL1 promoter or the GAL10 promoter, respectively. Correct clon...
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