New Fusion Trans-Activator Plasmids for Studying Signal Transduction
... fragment encoding residues
208 to 313 of rat c-Fos 12 into the bamh
I and EcoR
I sites of pFA-CMV. Our results ( figure
3...no acid residues 1-96 of human ATF2 14,15 was inserted
into the bamh
I and EcoR I sites of the pFA-CMV vector.
Consistent with previo...
Retroviral Vectors for Gene Delivery
Coding sequence for the enhanced green fluorescent protein (GFP) 9
was inserted between the EcoR I and bamh
I sites in pFB
and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line
HW293-A (unpublished data) was used for...
A New Lambda Vector for Mammalian Expression
To demonstrate expression levels in the pCMV-Script-EX vector, we inserted
the firefly luciferase gene into the bamh
I site of the MCS. This
same luciferase gene was inserted into the parental pCMV-Script vector.
These two constructs were used f...
Production of Full-Length, Biologically Active MEK1WT and MEK1CA
GST-MEK1 CA was purified as described. 2, 10
For expression in E. coli, the entire coding sequence of MEK1 CA
was inserted at the bamh
I site of the pGEX-2T plasmid. Epicurian Coli
XL1-Blue cells containing pGEX-MEK1 CA were induced by IPTG (1 mM) for
4 hours, when OD 600 reac...
Epitope-Tagging Vectors for Functional Analysis in Yeast
...AL1 or GAL10 promoter. To compare the promoter activity
under repressed and induced conditions, the luciferase gene was individually
cloned in the bamh
I site or the Not I site of pESC-TRP, so that
the gene is under the control of the GAL1 promoter or the GAL10 promoter,
respectively. Correct clon...