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BamH in Biological Technology

New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways

... fragment encoding residues 208 to 313 of rat c-Fos 12 into the bamh I and EcoR I sites of pFA-CMV. Our results ( figure 3...no acid residues 1-96 of human ATF2 14,15 was inserted into the bamh I and EcoR I sites of the pFA-CMV vector. Consistent with previo...

High-Titer Retroviral Vectors for Gene Delivery

... Fig.3 Coding sequence for the enhanced green fluorescent protein (GFP) 9 was inserted between the EcoR I and bamh I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) was used for...

A New Lambda Vector for Mammalian Expression

...ure 4 To demonstrate expression levels in the pCMV-Script-EX vector, we inserted the firefly luciferase gene into the bamh I site of the MCS. This same luciferase gene was inserted into the parental pCMV-Script vector. These two constructs were used f...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...ostatin]. GST-MEK1 CA was purified as described. 2, 10 For expression in E. coli, the entire coding sequence of MEK1 CA was inserted at the bamh I site of the pGEX-2T plasmid. Epicurian Coli XL1-Blue cells containing pGEX-MEK1 CA were induced by IPTG (1 mM) for 4 hours, when OD 600 reac...

Epitope-Tagging Vectors for Functional Analysis in Yeast

...AL1 or GAL10 promoter. To compare the promoter activity under repressed and induced conditions, the luciferase gene was individually cloned in the bamh I site or the Not I site of pESC-TRP, so that the gene is under the control of the GAL1 promoter or the GAL10 promoter, respectively. Correct clon...
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(Date:4/17/2014)... and pathogens that devastate honeybees in Europe, Asia and ... do not appear to be impacting native honeybee populations ... researchers., The invasive pests include including Nosema microsporidia ... honeybees appear to be resilient to these invasive pests, ... control pests in Europe, Asia and the United States ...
(Date:4/17/2014)... France have hit on a novel method to ... correct and most effective treatment possible., Kidney stones ... and developing world. If left untreated, apart from ... failure and other complications. In many patients treated ... a more effective pathological approach to diagnosis and ...
(Date:4/17/2014)... stark warning on the possible effects of gases such ... times that of carbon dioxide. Now a team of ... fully regenerable electron acceptors which helps explain why large ... being released to the atmosphere. However, there are worries ... into a vicious cycle to release large amounts of ...
Breaking Biology News(10 mins):East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3Methane climate change risk suggested by proof of redox cycling of humic substances 2
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