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Anneal in Biological News

'Virus chip' detects new virus in prostate tumors

...ray. Researchers then wash fluorescently tagged DNA from a sample of interest over the slide, and wherever the two sets of nucleic acid match up, they anneal to each other. The slide is then rinsed and visualized with a scanning laser microscope. The dots that have found a match glow with fluorescent light....
Anneal in Biological Technology

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...NA gene of L. monocytogenes, Listeria innocua, and Listeria seeligeri by the polymerase chain reaction (PCR)* using primers that anneal to conserved sequences, yielded multiple copies of gene fragments that, when evaluated by density gradient gel electrophoresis (...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

... II reverse transcriptase (Invitrogen) according to the manufacturers instructions. Gene-specific primers (GSPs) were designed to anneal near the 3' end of two mRNA transcripts and to generate PCR products 75300 base pairs long. Three GSP pairs amplify different ov...

RNA Amplification for Array Analysis

...s the typical probe orientation for microarrays? Oligonucleotides are single-stranded and therefore only anneal to their complement. Probes for microarrays are either generated from an RT reaction (cDNA) or from linear a...

Thermal Cycling Profile for Standard PCR

...itial heating of the PCR mixture for 2 minutes at 9495C is enough to completely denature complex genomic DNA so that the primers can anneal to the template as the reaction mix is cooled. If the template DNA is only partially denatured, it will tend to snap-back very quick...

Primer design

...designing the required primers (see figure below). Make primers that anneal to sequences in exons on both sides of an intron (Panel 1). With these primers, any product amplified from genomic DNA will be m...

QIAGEN Multiplex PCR Handbook

...s of the primer mix. 9. Amplification of pseudogene sequences Primers may anneal to pseudogene sequences and additional PCR products may be amplified. Review primer design to avoid detection of pseudog...

Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates

...he renaturation of the template and the formation of secondary structures within each strand before the primers have a chance to anneal to their specific template sites. These properties of GC-rich DNA sequences lead to inefficient primer-template annealing and in...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... that are complementary to the single-stranded tails of the pCAL-n-EK vector. The vector and insert DNA are combined, allowed to anneal at room temperature and transformed into highly competent bacterial host cells. The resultant colonies can then be screened for ...

High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli

...ct is purified, then treated with Pfu DNA polymerase in the presence of dATP. The vector and insert DNA are combined, allowed to anneal at room temperature, and transformed into highly competent bacterial host cells. The resultant colonies can then be screened for...

Map and Link Human Genetic Disorders with SSLP Analysis

...articular location. 1 Often these markers are (CA)n repeat polymorphisms. 1 These polymorphisms can easily be genotyped by PCR with primers that anneal to single-copy DNA, which flanks the repetitive element. Genotyping, using microsatellite markers, at specific locations throughout the genome...
Anneal in Biological Definition

Genetic recombination

...lowing the nicked strand to pull apart from its complementary strand and anneal to one strand of the double helix on the opposite chromatid. A second nic...k allows the unannealed strand in the second double helix to pull apart and anneal to the remaining strand in the first, forming a structure known as a cross...

Polymerase chain reaction

...and end of the DNA fragment to be amplified. They anneal (adhere) to the DNA template at these starting an...ned as the temperature below which the primer will anneal to the DNA template and above which the primer w...th of the primer. Primers that are too short would anneal at several positions on a long DNA template, whi...

Primer

...the end of the DNA fragment to be amplified. They anneal (adhere) to the DNA template at these starting an...ned as the temperature below which the primer will anneal to the DNA template and above which the primer wil...th of the primer. Primers that are too short would anneal at several positions on a long DNA template, which...
Anneal in Biological Dictionary

Anneal

Annealing , in metallurgy and materials science, is a heat treatment wherein a ... A full anneal typically results in the second most ductile state a metal can ... For other uses, see annealing . Annealing is a process of slowly cooling ... Annealing · Chemical vapor deposition · Glas...

Sticky ends

...ging, ... You'll come to a sticky end . Cohesive. Benight. complimentary. molecule. DNA ... Name the bonds that are formed when sticky ends anneal and those that are formed when blunt ends anneal. This results in both ends having a single stranded area called sticky ends . ... this spa...
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