A fresh look at existing therapies: Researchers explore ways to teach approved drugs new tricks

... Manuel Hidalgo, M.D., Ph.D., suspected that pancreatic cancer − a disease in which EGFR-targeted therapies have proved to be of use, but where amplifications or mutations in the EGFR gene are rare − is an example of a cancer driven by pathway addiction. The problem, however, is that no one knows what ...

New Mouse Model to Aid in Cancer-causing Genes Hunt

...mphoma, or T-ALL, and the cancer cells showed rampant genome instability resulting in intricate pattern of mutations, chromosomal rearrangements, gene amplifications and gene deletions similar to those in human solid tumors. Researchers characterized these mouse tumors by performing high-resolution array-C...

Amplifications in Medical Technology

Breast Cancer and HER-2/neu: Diagnostic Tools for Targeted Therapy

...plication and cell division. Her-2/neu therefore falls into the class of proto-oncogenes. A key discovery is that some 2530% of breast cancers carry amplifications (multiple copies) of the Her-2/neu gene, which lead to overexpression of Her-2/neu protein. The over-abundance of Her-2/neu receptor accelerates the ...

Amplifications in Biological News

Genome changes tracked during multiple myeloma initiation, progression and treatment

...iles of outcome-annotated MM clinical specimens. The researchers discovered a high level of molecular heterogeneity typified by many new recurrent amplifications and deletions that point to a large number of yet-to-be-discovered oncogenes and tumor suppressor genes. These amplification and deletion patterns def...

Amplifications in Biological Technology

The TripleMaster PCR System for three different amplifications

Christian Rohrer, Eppendorf AG Introduction Standard PCR components (Taq polymerase and reaction buffers) are used for the general amplification of relatively short DNA fragments ...

ZyGEM Releases Application Note Showing its DNA Extraction Kits Rapidly Produce High Yields of Quality DNA

...evant segment of DNA using PCR reagents supplied in the kits and also with an external amplification system. The prep GEM(TM) Tissue extractions and amplifications yielded superior results based on both qualitative and quantitative PCR comparisons of the amplified DNA. "This new application note focusing on ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...ificity of FFPE DNA amplification from GenomiPhi amplifications by performing conventional PCR on select human g...s of mean intensities from DOP-PCR and GenomiPhi amplifications showed hybridization characteristics to be simil...om GenomiPhi amplifications. DOP-PCR and GenomiPhi amplifications showed hybridization characteristics to be similar...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

... produced similar DNA yields, sensitivity and performance. We also showed that BAC DNA generated by the GenomiPhi method detected all 8p12 chromosomal amplifications that were also detected by the DOP-PCR method in an MDA-MB-134 breast cancer line. References 1. Mamo...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

... in serial dilutions of cDNA derived from a pooled sample (see above) was measured (Figure 2A). Figure 2B shows C T ) values of these amplifications plotted against the logarithm of the relative initial amount of the cDNA. Theoretically, there should be an inverse linear relat...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

...d at 20C in a constant-temperature freezer. Again, only the ACTB-derived transcripts were amplified (Figure 1B). Traces for late amplifications (C T > 34) of the 128 bp primer set represent primer-dimers and not specific product, as determined by melt-curve and gel analy...

Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A

...em, and continuous SYBR Green I fluorescence monitoring was performed according to Bio-Rad protocols. Cycling parameters for all amplifications were 30 sec at 95C, 30 sec at 55C, and 30 sec at 72C, for 30 cycles. Amplified promoter fragments were subcloned into the T/A cl...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

...nded heavily on the amplification efficiencies of both targets. Large differences required high- (>90%) and equal-efficiency PCR amplifications of both genes. Amplification efficiencies of the GAPDH and α-tubulin plasmids were determined prior to multiplexing and fou...

Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations

...TTAGTGCTCCCT- 3) which contains a 40 base-pair (bp) GC clamp. 9 amplifications were carried out using 100 ng DNA template in 1x PCR buffer (Per.... Mutant and wild-type -thalassemia DNA were isolated from blood. amplifications were carried out using 100 ng DNA template in 1x PCR buffer, 0.2...

Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System

...ned this method by using multiplex PCR* amplifications combined with Multiplex Denaturing Grad...ere evaluated (Michiels et al. 1996). 3 amplifications were carried out in 20 l reaction mixtur...arable efficiencies, different exon PCR amplifications were combined. Moreover, to make sure t...

Amplifications in Biological Products

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:...Designed to degrade PCR products from previous PCR amplifications without degrading native nucleic acid templates, w...pecifically degrade PCR products from previous PCR amplifications in which dUTP has been incorporated, without degra...ntaining DNA templates. Products from previous PCR amplifications are eliminated by excising uracil residues using U...
Company:Applied Biosystems